本實驗將利用臭氧活化固定柚皮苷於幾丁聚醣表面，藉由臭氧之強氧化性，於劑材表面產生過氧化物並利用氧化還原的方式產生氧的自由基於材料表面，進而達到將柚皮苷固定於基材表面之目的，利用臭氧改質可以有效避免因交聯劑產生的細胞毒性。柚皮苷為天然黃酮類，是一種HMG-CoA之抑制劑，發現有治療骨質疏鬆之效果，且能有效的促進骨細胞之生長，但高濃度之柚皮苷會產生細胞毒性，利用臭氧活化固定柚皮苷，改善釋放初期之快速釋放會導致藥物於短時間內累積濃度過高，進而產生的細胞毒性之現象。
釋放實驗結果，經臭氧活化固定柚皮苷之薄膜浸泡於PBS中，能有效控制釋放速率及濃度。體外實驗，以吸附的方式固定柚皮苷，7F2細胞活性明顯下降，UMR活性雖未出現明顯抑制的現象，但皆出現抑制細胞延展的現象，對於7F2及UMR的ALPase表現明顯有抑制的現象產生。經臭氧改質之幾丁聚醣薄膜，因控制柚皮苷之釋放速率及濃度，皆能促進7F2與UMR活性，對於ALPase表現亦有明顯增加的增加，證明攜帶柚皮苷之幾丁聚醣薄膜具有誘導骨生成作用。

The ozone oxidation can easily produce peroxides with free radicals for the surface modification on biomaterials. This process would be highly efficient and without any toxicity from crosslinkers. In this research, naringin, a HMG-CoA reductase inhibitor which can promote bone formation, was immobilized onto chitosan film by using the ozone activation process. At first, chitosan films were treated by the ozone activation to produce peroxides for the following immobilization of naringin. The amounts of peroxides produced by ozone treatment were quantified and optimized by the iodide assay.
In the in vitro delivery, the chitosan substrate with immobilized naringin was immersed in PBS and released amounts of naringin were measured by UV. The results showed that the ozone treatment can decrease the releasing rate and final concentration of naringin. The culture of osteoblast-like cells and osteoblasts showed that cell activity and proliferation were promoted by the immobilized naringin on chitosan substrates without any cytotoxicity. The early osteoblastic differentiation, ALPase expression, would be also enhanced. The results in this research demonstrated that naringin was efficiently immobilized onto chitosan substrates with the ozone treatment.

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