癌症是當今全球性健康議題，迫切需要在癌症治療方面取得進展。然而環境中菌株耐藥性的議題也受到各界關注，因此尋找新的抗菌解決方案變得至關重要。此外，近年來病毒感染對公共衛生和個人健康帶來嚴重威脅。在這些領域中，功能性生物材料的應用能夠提供新的解決方案，但是都僅限於單一面向或功能性。在本論文中，我們成功開發一種含銀金屬的超分子聚合物，同時具備抗癌、抗菌和抗病毒等三大面向的應用潛力。此超分子聚合物（AgCyPEG）是透過銀離子與胞嘧啶官能化聚合物進行配位反應而得，其所得的產物能在水溶液中形成球型奈米凝膠並展現出許多特異的物理特性，如自發性藍色螢光特性、高靈敏的pH響應性及良好可控的銀離子釋放。隨後我們將AgCyPEG進一步延展至溶血、抗癌、抗菌及抗病毒等醫學應用的評估。首先，溶血測試證實AgCyPEG具有良好的抗溶血特性及結構穩定性。在抗癌方面，AgCyPEG展現出高效選擇性癌細胞毒殺的能力，同時不會影響正常細胞的生物活性。細胞毒殺機制的實驗亦證實AgCyPEG能有效被吞噬至癌細胞內部並導致大量的細胞凋亡。在抗菌方面，AgCyPEG對革蘭氏陽性菌及革蘭氏陰性菌表現出優異的抑制活性，即使AgCyPEG中的銀含量極低 （≤ 3 %）。此外，AgCyPEG在抗病毒方面也表現出對禽流感病毒（H1N1及H5N1）顯著的抗病毒活性，其結果證實AgCyPEG具有作為預防性抗病毒試劑的潛力。總體來說，本次研究結果表明此新興開發的系統具有多面向的治療特性，未來極具潛力拓展至體內與臨床研究的評估。

Cancer is a global and serious health issue today, with an urgent need for advancements in cancer treatment. The growing problem of antibiotic resistant in the environment has also raised concerns, promoting the importance of finding new antibacterial solutions. In additional, viral infections have become serious threats to public health and personal health in recent years. In these fields, functional biomaterials offer potential solutions, although they have been limited to only one aspect or functionality. In this study, we successfully developed a silver-containing supramolecular polymer with potential applications in anticancer, antibacterial, and antiviral treatments. This supramolecular polymer, AgCyPEG, was synthesized through the coordination reaction between silver ion and cytosine-functionalized polymer. The resulting polymer self-assembles into spherical nanogels in aqueous solutions and exhibits several unique physical properties, such as spontaneous blue fluorescence, highly sensitive pH-responsiveness, and well-controlled release of silver ions. Subsequently, we further evaluated AgCyPEG in biomedical applications, including hemolysis, anticancer, antibacterial, and antiviral activities. The hemolysis test confirmed the anti-hemolytic properties and structural stability of AgCyPEG. In terms of anticancer properties, AgCyPEG demonstrated highly selective cytotoxicity towards cancer cells while preserving the viability of normal cells. The studies of cytotoxic mechanism revealed that AgCyPEG was efficiently internalized by cancer cells, leading to the induction of massive cell apoptosis. In antibacterial experiments, AgCyPEG showed remarkable inhibitory activity against both Gram-positive and Gram-negative bacteria, even with extremely low silver content （≤ 3 %） in AgCyPEG. Moreover, AgCyPEG exhibited significant antiviral activity against avian influenza viruses （H1N1 and H5N1）, indicating its potential as a preventive antiviral agent. Overall, our findings demonstrate that this newly developed system possesses multifaceted therapeutic capabilities and holds great potential for further evaluation in in vivo and clinical studies.

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