本研究的目的是開發一新穎製程技術，製造全透明非平面微流體晶片，未來可用來製造仿生人體組織結構，運用在生物領域的細胞養殖上或是體內化學領域藥劑傳輸的研究上。在本製程中，將利用3D列印技術、表面拋光技術、PDMS澆注技術、以及二階段化學溶液溶解技術。
　　本論文分成兩階段，第一階段是利用3D列印中的熱熔擠製成型技術(Fused Deposition Modeling)製作非平面之丙烯腈丁二烯苯乙烯ABS微流道模具，並利用自製的表面拋光系統減少3D列印模具的表面粗糙度。第一階段的研究重點在於討論數個模具及列印參數對於製造非平面模具的影響。參數包括模具設計、列印的支撐材設定及選擇、如何解決翹曲問題以及化學拋光系統對非平面模具表面的影響。在第二階段中將利用圍牆結構及聚二甲基矽氧烷(PDMS)澆注技術，對第一階段的3D列印模具進行結構複製，最後步驟是利用化學溶液溶解技術及超音波輔助溶解技術溶解3D列印非平面模具，即可完成全透明非平面PDMS微流體晶片。
　　本研究的結論為：(1)此新穎的製程技術可用來成功製造全透明且非平面的微流道晶片;(2)此製程的必要步驟包括:3D列印非平面模具、化學拋光處理、二階段化學溶液溶解技術、PDMS翻模技術，此研究成果讓發展幾十年的生醫晶片由平面結構延伸成非平面結構，讓生醫晶片融入更多的非平面微流體結構；(3)超音波輔助溶解技術可以加速且完全溶解PDMS中的ABS，是本製程中最關鍵的一項技術;(4)三維度微流道結構相較平面結構更能模擬微觀人體組織，透過本研究所開發之製程，可大幅提高三維度微流道晶片設計之複雜性，更能符合臨床實驗的模擬結果，對往後醫療領域發展方面有重大的貢獻。

　　The aim of this research is to develop a novel manufacturing process to create fully transparent and nonplanar microfluidic chip, which can be used as bionic human tissue structure for applications in the biology cell culture or chemical medicine transportation fields. Multiple manufacturing processes would be used to crate nonplanar microfluidic chip in this article, including 3D printing technique, solvent surface treatment technique, casting technique, and two steps solvent dissolution technique.
　　This thesis can be separated into two major stages: the goal of the first stage is to fabricate nonplanar microfluidic mold by using The Fused Deposition Modeling (FDM) 3D printing technique to fabricate nonplanar thermoplastic mold inserts with material of acrylonitrile butadiene styrene (ABS). Using a home-built solvent polish system to minimize the surface roughness of nonplanar mold. The influence from several parameters were discussed in the first stage, including mold design, support material setting and choose, bending problem and solvent surface treatment performance. The second stage of the extension form the first stage, and the goal is to nonplanar microfluidic chip. The fabrication process included fabrication of nonplanar microfluidic mold insert, fence structure and PDMS casting, solvent dissolution technique and sonication-assisted dissolution technique.
　　The conclusions from this research are: (1) this novel fabrication process can be used to fabricate fully transparent, nonplanar microfluidic chip; (2) Multiple manufacturing processes would be used to crate nonplanar microfluidic chip in this article, including 3D printing technique, solvent polish system, two step dissolution technique and PDMS casting. This study can create fully transparent, nonplanar PDMS microfluidic chip for more application field like biology and medical; (3) Sonication-assisted dissolution technique which is a major technique of this study can help us to fabricate nonplanar microfluidic chip; (4) With this technique, truly three-dimensional, transparent, and more complicated microfluidic platforms can be created such as artificial organs, human tissue, and vascular vessels, and used for more in-vitro experiments.

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