當傷口太深或太大時則不易修復，形成由纖維母細胞所構成疤痕組織。透過人工皮取代疤痕位置是目前臨床主要使用的解決方式。材料細菌纖維素是一種天然的纖維以應用在許多醫療的應用上，因為與其他種合成纖維相比，細菌纖維素有很好的生物降解性以及再生性。此外白藜蘆醇是一種天然的多酚類，具備抗菌、抗真菌、抗發炎以及抗氧化的能力，幫助對付皮膚疾病。在本論文中，我們試著利用白藜蘆醇結合細菌纖維素覆蓋傷口，並與已使用在傷口修復明膠與膠原蛋白I結合膜(GC-I)進行比較。我們透過掃描式電子顯微鏡觀察，膜的表面上發現白藜蘆醇的顆粒。細菌纖維素的孔徑約為100~200奈米比GC-I形成的孔徑小。由結果可知，在體外試驗中，將含有白藜蘆醇的細菌纖維素膜使用人工唾液進行藥物釋放，可在10分鐘後釋放出來，幫助在貼附傷口的一開始，便提供抑制發炎反應的環境。體外測試，將人體脂肪幹細胞種植在生物膜上培養7與14天之後，使用免疫螢光染色法進行染色，確定有很好的生物相容性。此外拍照觀察傷口外觀以及透過Masson’s trichrome stain 確認使用膜覆蓋皮膚後的重建狀況。根據我們的實驗結果，使用含有白藜蘆醇的細菌纖維素因為具有很好的生物相容性以及調控發炎反應的作用，能夠提供受損皮膚一個較好的環境進行皮層的修復。

The scar formation created for covering the wound area by fibroblast, as the depth and area too extensive is difficult to repair. A promising solution to this problem is the use of skin substitutes to replace scar tissue in clinical treatments. The material, bacterial cellulose, is very attractive for medical applications due to its biodegradability and renewability compared to synthetic fibers. Resveratrol is a natural polyphenol which has antibacterial, antifungal, anti-inflammatory, and antioxidant capabilities, enabling it to act as drugs for the treatment of skin diseases. In this work, we tried to reduce probability of infectious by dressing the resveratrol contained cellulose membrane on various wounds and comparing the results to that of GC-I membrane which was reported to be used for wound healing. We observed the particles of resveratrol and the surface morphology of the membrane with a scanning electron microscope. The porous size of the cellulose membrane was about 100~200nm smaller than that of the GC-I membrane. Further experiments showed that the resveratrol can release from the membrane after 10 minutes in artificial tissue fluid in vitro, indicating that the environment for inflammation response was resisted immediately after replaced by membrane. Immuno-stain in vitro also showed that the cells on membranes for 7, and 14 days had great biocompatibility. Furthermore, the evidences of visual observation and Masson’s trichrome stain clearly indicate that the morphology of membranes-covered skin had reconstructed. According to our experimental results, resveratrol contained bacterial cellulose membranes can offer damaged skin a better environment for epithelial regeneration due to its excellent biocompatibility and inflammation regulator conjugating.

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