二級胺常見於自然界及生物體內，例如生物細胞內利用蛋白質離胺酸甲基轉移酶和脫甲基酶之間的平衡反應進行調節蛋白質中離胺酸甲基化的基質和程度（單甲基、二甲基、三甲基）。這種甲基酶反應平衡的失調通常與神經系統疾病、發育異常或癌症等疾病狀態相關。因此，本文設計了可用於標定二級胺的螢光響應探針：1,2,3–三嗪–BODIPY衍生物。利用簡單且溫和的反應條件，二級胺可以與1,2,3–三嗪發生反應，藉由破壞螢光淬滅基團——1,2,3–三嗪的機制，誘使BODIPY的螢光團發光。在一系列分子中，p-TZ-H、p-TZ-Et及m-TZ-Et與二級胺（N-甲基丁胺、吡咯烷）反應後亮度明顯提高。因此我們認為1,2,3-三嗪-BODIPY衍生物未來有機會用於二級胺基酸的標定上。

Secondary amines are commonly found in nature and organisms. For example, the balance reaction between protein lysine methyltransferase and demethylase is used in biological cells to regulate the substrate and degree of lysine methylation in proteins (monomethyl, dimethyl, trimethyl). Disturbances in the balance of this methylase reaction are often associated with disease states such as neurological disorders, developmental abnormalities, or cancer. Therefore, this project designed a series of 1,2,3-triazine based BODIPY derivatives as a fluorescent response probe that can be used to calibrate secondary amines. By using simple and mild reaction conditions, secondary amines can react with 1,2,3-triazine to induce fluorescent intensity of BODIPY by destroying the mechanism of the fluorescent quenching group 1,2,3-triazine. Among a series of molecules, p-TZ-H, p-TZ-Et and m-TZ-Et significantly increased their brightness after reacting with secondary amines (N-methylbutylamine, pyrrolidine). Therefore, we believe that 1,2,3-triazine-BODIPY derivatives have the opportunity to be used in the calibration of secondary amino acids in the future.

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