研究生: |
藺莛恩 Ting-An Lin |
---|---|
論文名稱: |
不同矽含量生物活性玻璃對細胞毒性與生物活性影響之研究 Influence of Si concentration on cytotoxicity and bioactivity of spray dried bioactive glass |
指導教授: |
施劭儒
Shao-Ju Shih |
口試委員: |
王丞浩
Chen-Hao Wang 鍾仁傑 Ren-Jie Chung 吳孟晃 Meng-Huang Wu |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2018 |
畢業學年度: | 106 |
語文別: | 中文 |
論文頁數: | 95 |
中文關鍵詞: | 生物活性玻璃 、噴霧乾燥法 、生物活性 、細胞毒性 |
外文關鍵詞: | bioactive glass, spray dry, bioactivity, cytotoxicity |
相關次數: | 點閱:237 下載:10 |
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隨著高齡化社會的來臨,老年人普遍有骨質疏鬆的問題,一旦發生骨折意外將不易修復,因此生物活性玻璃(Bioactive glass, BG) 因為具有良好的生物活性與骨傳導性,而受到許多關注。BG的製程有許多種,例如:傳統玻璃製程、溶膠-凝膠法、噴霧熱解法等等,但是傳統玻璃製程的純度不高,溶膠-凝膠法製程時間過長,而噴霧熱解法製程的粒徑不易控制。噴霧乾燥法(Spray dry, SD)法式一個較為新穎的生物活性玻璃製程,此法提供了一個純度高且能夠量產(i.e. 1kg/h)的方法。實驗中成功製備Si-Ca-P系統的生物活性玻璃,依照SiO2的含量分為58S、68S與76S的粉體及燒結後的塊材。
利用X光繞射技術(XRD)鑑定其相結構,使用電子顯微鏡(SEM)觀察其表面形貌。在體外測試中,將BG浸泡於人體模擬體液十二小時後,測量其pH值變化,並利用XRD與SEM測量其生物活性,最後進行MTT測試其細胞毒性。由實驗結果發現,對於生物活性(相同時間的HA生成量)而言,58S最佳,68S其次,而76S生物活性最差;但是對於細胞毒性而言,卻是76S細胞毒性最低,58S細胞毒性最高的結果。細胞毒性主要是因為過量的鈣離子釋放,造成細胞死亡,因此若將粉體燒結成塊材,鈣離子釋放較慢,使其細胞毒性降低。
The problem of the aging society is becoming more and more obvious. The most serious problem is the issue of osteoporosis associated with population aging. Once the bone fracture accident happens, it will not be easy to repair for the elderly people. Thus, bioactive glass (BG) has attracted much attention due to its excellent bioactivity and osteoconductivity properties. There are many kinds of processes to synthesize the BG, such as traditional glass process, sol-gel method, and spray pyrolysis. However, the BG powder in the traditional glass process has the problem of the low purity; in the sol-gel method need a long time to compose the BG powder; it is difficult to control the particle size using spray pyrolysis. For the Spray Dry (SD) method, which is a newly developed bioactive glass process that can provide a high purity and mass production (i.e. 1kg/h).
In this research, the powders and bulks of the Si-Ca-P system bioactive glass, 58S, 68S and 76S were successfully prepared by SD. The phase structure was characterized by X-ray diffraction (XRD). The surface morphology was observed using an electron microscope (SEM). The in-vitro bioactive tests were measured by XRD and SEM, and finally, the cytotoxicity was tested by MTT. The results indicated that 58S is the best sample for the bioactivity test (HA growth in the same soaking time) followed by 68S and 76S. However, the results of the cytotoxicity test are different from the bioactivity test, it is shown that 76S is the least cytotoxic in all the sample. In addition, 58S is the most cytotoxic sample due to a lot of the Ca2+ ions content in the 58S. The excess of Ca2+ ions release leads to the cell apoptosis. In order to overcome this problem to decrease the cytotoxicity, sintering the powder into a bulk can reduce the release rate of Ca2+ ions.
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