簡易檢索 / 詳目顯示

研究生: 黃俊凱
Jun-Kai Huang
論文名稱: 波長6.3µm遠紅外線照射水後對於綠茶萃取與線蟲生長的實驗差異之研究
Effects of water irradiated with far infrared wave having 6.3 µm wavelength on the extraction of green tea ingredients and growth of Caenorhabditis elegans
指導教授: 林舜天
Shun-Tian Lin
口試委員: 林舜天
Shun-Tian Lin
丘群
Chun Chiu
胡泉凌
CHUAN-LING HU
學位類別: 碩士
Master
系所名稱: 工程學院 - 機械工程系
Department of Mechanical Engineering
論文出版年: 2018
畢業學年度: 106
語文別: 中文
論文頁數: 58
中文關鍵詞: 遠紅外線多酚自由基咖啡因秀麗隱桿線蟲
外文關鍵詞: far infrared, polyphenols, free radical, caffeine, Caenorhabditis elegans
相關次數: 點閱:279下載:0
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 水在FTIR的檢測中,O-H鍵在1640cm-1是做彎曲的運動。接近石墨烯產生的遠紅外線(6.3 μm的波長或波數1587cm-1)。預期照射遠紅外線可讓O-H鍵共振,產生物理和化學現象的差異,以此作為本研究綠茶萃取和線蟲生長之實驗。
    照射遠紅外線的水對於綠茶萃取析出量都有提高,冷泡在25度C泡1小時,多酚含量增加19.30%,自由基清除率增加19.01%,咖啡因含量7.59%。相較之下,熱泡在80度C泡1分鐘,多酚含量增加15.22%,自由基清除沒增加,咖啡因含量19.87%,而熱泡所需的時間比冷泡時間少就能有明顯差異。使用遠紅外線照射的水餵食,生長在25度C培養箱中的線蟲明顯實驗組比對照組壽命增加16.4%,平均長度增長8.42%。


    Water molecules show a characteristic O-H bond bending peak at 1640 cm-1 in FTIR spectrum, which is close to the far infrared ray generated by graphene (wavelength of 6.3 μm, or wavenumber 1587 cm-1). It is thus expected that the far infrared ray irradiation can cause the resonance of the O-H bond, which can possibly cause the differences in various physical and chemical phenomena. In this study, the effects of far infrared irradiated water on the extraction of ingredients from green tea and the growth of Caenorhabditis elegans are studied.
    It was found that irradiation of the far infrared ray at 25 ° C for 1 hour on water caused the increases of extraction rates of polyphenol, free radical scavenging rate, and caffeine by 19.3%、19.0% and 7.6%, respectively. In comparison, soaking the green tea in hot water at 80 ° C for 1 min resulted in the increased extraction rates of polyphenol, free radical scavenging rate, and caffeine increased by 15.2%、0% and 19.9%, respectively. Fed with far infrared irradiated water, the life of Caenorhabditis elegans in the 25 °C incubator significantly increased by 16.4% and their average length increased by 8.4 %.

    摘要 I Abstract II 致謝 III 目錄 IV 圖目錄 VII 表目錄 IX 第一章 前言 1 1.1研究動機 1 1.2研究目的 1 第二章 文獻探討 2 2.1 遠紅外線簡介[1,2] 2 2.1.1 遠紅外線加熱原理[1,2] 3 2.2 石墨與石墨烯簡介 4 2.2.1石墨 4 2.2.2石墨烯 4 2.3 綠茶簡介 7 2.3.1 多酚(Polyphenol) 7 2.3.2 自由基(Free radical) 9 2.3.3 咖啡因(Caffeine) 11 2.4 秀麗隱桿線蟲簡介 12 2.5 傅立葉紅外線光譜儀(Fourier transform infrared spectrometer) 15 2.6 紫外光/可見光分光光譜儀[37] 17 第三章 實驗方法與步驟 19 3.1 實驗流程與材料設備 19 3.2 綠茶實驗 21 3.3 線蟲實驗 23 第四章 結果與討論 27 4.1 傅立葉轉換遠紅外線光譜儀分析 27 4.2 綠茶實驗分析 28 4.2.1 多酚含量 28 4.2.2 自由基清除率 30 4.2.3 咖啡因含量 32 4.3 線蟲實驗分析 36 第五章 結論 41 參考文獻 42

    [1] 陳林照,功能性抗電磁波/遠紅外線複合梭織物之織造技術及其特性分析,逢甲大學紡織工程研究所碩士論文,民國98年。
    [2] 邱垂豪,遠紅外線功能之聚乙烯醇纖維,逢甲大學紡織工程研究所碩士論文,民國91年。
    [3] 蕭清松,遠紅外線陶瓷輻射體的製作,陶業季刊,第21-22頁,1995。
    [4] K. Yamashita , “The Effects of the Far-Infrared Ray (FIR) Energy Radiation on Living Body”, International Journal of Biometeorology, 14, 271-302, 2012.
    [5] P.R.Wallace, “The band theory of graphite”, Physical Review, 71(9), 622-634, 1947.
    [6] K. S. Novoselov, A. K. Geim, S. V. Morozov, D. Jiang, Y. Zhang, S. V. Dubonos, A. A. Firsov, “ Electric field effect in atomically thin carbon films”, science, 306(5696), 666-669, 2004.
    [7] A. K. Geim, K. S. Novoselov, “The rise of graphene”, Nature Mater, 6(3), 183-191, 2007.
    [8] C. Lee, X. Wei, J. W. Kysar, J. Hone, “ Measurement of the elastic properties and intrinsic strength of monolayergraphene”, science,
    321(5887), 385-388, 2008.
    [9] R. R. Nair, P. Blake, A. N. Grigorenko, K. S. Novoselov, T. J. Booth, T. Stauber, N. M. R. Peres, A. K. Geim, “Fine Structure Constant Defines Visual Transparency of Graphene”, Science, 320(5881), 1308, 2008.
    [10] K. S. Novoselov, A. K. Geim, S. V. Morozov, D. Jiang, M. I. Katsnelson, I. V. Grigorieva, S. V. Dubonos, A. A. Firsov, “Two-dimensional gas of massless Dirac fermions in graphene”, Nature, 438(7065), 197-200, 2005.
    [11] N. Ullah, M. Ahmad, A. Aslam, M. A. Tahir, M. Aftab, N. Bibi, S. Ahmad, “Green tea phytocompounds as anticancer: A review”, Asian Pacific Journal of Tropical Disease , 6(4), 330-336, 2016.
    [12] A. Komori, J. Yatsunami, S. Okabe, S. Abe, K. Hara, M. Suganuma, S. J. Kim, H. Fujiki,“Anticarcinogenic Activity of Green Tea Polyphenols”, Japanese Journal of Clinical Oncology, 23(3), 186–190, 1993.
    [13] D. Harman, “Aging: a theory based on free radical and radiation chemistry” , Journal of Gerontology, 11(3), 298–300, 1956.
    [14] M. Valko, D. Leibfritz, J. Moncol, et al., “Free radicals and antioxidants in normal physiological functions and human disease”, International journal of biochemistry &cell biology, 39(1), 44-84, 2006.
    [15] B. Caballero, P. Finglas, F. Toldra, “ Encyclopedia of Food and Health”, Elsevier Science, 561, 2015.
    [16] A. Nehlig, J. L. Daval, G. Debry, "Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects", Brain Research Reviews, 17 (2), 139–170, 1992.
    [17] V. Gupta, L. A. Lipsitz, "Orthostatic hypotension in the elderly: diagnosis and treatment", The American Journal of Medicine,
    120 (10), 841–847, 2007.
    [18] S. Bolton,"Caffeine: Psychological Effects, Use and Abuse" , Orthomolecular Psychiatry, 10 (3), 202–211, 1981.
    [19] G. Grosso, A. Micek, S. Castellano, A. Pajak, F. Galvano, "Coffee, tea, caffeine and risk of depression: A systematic review and dose-response meta-analysis of observational studies", Molecular Nutrition & Food Research, 60 (1), 223–34, 2016.
    [20] S. Brenner, W. B. Wood, “The nematode Caenorhabditis elegans”, Cold Spring Harbor, 1988.
    [21] S. Brenner, “The worm's turn”, Current Biology, 12(21), R713, 2002.
    [22] M. A. Félix, “RNA interference in nematodes and the chance that favored Sydney Brenner”, Journal of Biology, 7(9), 34, 2008.
    [23] M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, D. C. Prasher, “Green fluorescent protein as a marker for gene expression”, Science, 263(5148), 802-805, 1994.
    [24] T. Boulin, J. F. Etchberger, O. Hobert, “Reporter gene fusions”, WormBook, ,1-23,2006.
    [25] E. H. Feinberg, M. K. Vanhoven, A. Bendesky, G. Wang, R. D. Fetter et al., “GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems” Neuron, 57(3), 353-363, 2008.
    [26] J. Sulston, M. Dew, S. Brenner, “Dopaminergic neurons in the nematode Caenorhabditis elegans”, Journal of Comparative Neurology, 163(2), 215-226, 1975.
    [27] J. Kimble, D. Hirsh, “The post-embryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans” Developmental Biology, 70(2), 396-417, 1979.
    [28] J. E. Sulston, E. Schierenberg, J. G. White, J. N. Thomson, “The embryonic cell lineage of the nematode Caenorhabditis elegans”, Developmental Biology, 100(1), 64-119, 1983.
    [29] J. G. White, E. Southgate, J. N. Thomson, S. Brenner, “The structure of the nervous system of Caenorhabditis elegans”, Philosophical Transactions of the Royal Society B: Biological Sciences, 314(1165), 1-340, 1986.
    [30] C. elegans Sequencing Consortium, “Genome sequence of the nematode C. elegans: a platform for investigating biology” Science, 282(5396), 2012-2018, 1998.
    [31] D. D. Shaye, I. Greenwald, “OrthoList: a compendium of C. elegans genes with human orthologs”, PLoS ONE, 6(5), e20085, 2011.
    [32] T. Kaletta, M. O. Hengartner, “Finding function in novel targets: C. elegans as a model organism”, Nature Reviews Drug Discovery, 5(5), 387-398, 2006.
    [33] E. Culetto, D. B. Sattelle., “A role for Caenorhabditis elegans in understanding the function and interactions of human disease genes”, Human Molecular Genetics, 9(6), 869-877, 2000.
    [34] P. Griffiths, J. A. Hasseth, “Fourier Transform Infrared Spectrometry 2nd”, Wiley-Blackwell, 2007.
    [35] “The Infracord double-beam spectrophotometer”, Clinical Science, 35 (33), 74, 1957.
    [36] “Fourier-transform infrared spectroscopy’’ , Wikipedia,http://en.wikipedia.org/.
    [37] “ultraviolet–visible spectroscopy’’ , Wikipedia,http://en.wikipedia.org/.
    [38] 胡泉凌,紅外線面光源產生裝置,中華民國發明專利號I566803,2014。
    [39] J. J. Max, C. Chapados, “Isotope effects in liquid water by infrared spectroscopy. III. H2O and D2O spectra from 6000 to 0 cm−1”, Journal of Chemical Physics, 131(18), 184505, 2009.
    [40] V. L. Singleton, R. Orthofer, R. M. Lamuela-Raventós, “Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent”, Methods Enzymol, 299(1), 152-178, 1999.
    [41] W. Brand-Williams, M. E. Cuvelier, C. Berset, “Use of a free radical method to evaluate antioxidant activity”, LWT - Food Science and Technology, 28(1), 25-30, 1995.
    [42] S. Ahmad Bhawani, S. S. Fong, M. N. Mohamad Ibrahim, “Spectrophotometric Analysis of Caffeine,” International Journal of Analytical Chemistry, 2015, 1-7, 2015.

    無法下載圖示 全文公開日期 2023/07/25 (校內網路)
    全文公開日期 本全文未授權公開 (校外網路)
    全文公開日期 本全文未授權公開 (國家圖書館:臺灣博碩士論文系統)
    QR CODE