簡易檢索 / 詳目顯示

研究生: 魏可兒
Colette Ouedraogo
論文名稱: Microbial Induced Calcite Precipitation (MICP) on Taipei Silty Clay
Microbial Induced Calcite Precipitation (MICP) on Taipei Silty Clay
指導教授: 鄧福宸
Fuchen Teng
口試委員: 蔡伸隆
Tsai, Shen-Long
陳韋志
陳韋志
學位類別: 碩士
Master
系所名稱: 工程學院 - 營建工程系
Department of Civil and Construction Engineering
論文出版年: 2019
畢業學年度: 107
語文別: 英文
論文頁數: 128
中文關鍵詞: MICPSporosarcina pasteuriiUrease activityBiocementation
外文關鍵詞: MICP, Sporosarcina pasteurii, Urease activity, Biocementation
相關次數: 點閱:171下載:0
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • Microbial Induced Calcite Precipitation (MICP) is a new technique of improving the engineering properties of soil, a multidisciplinary technique that involves biology, chemistry and soil mechanics. This technique involves the hydrolysis of urea by urease bacteria enzyme into carbonate ions and ammonium ions that precipitate in form of calcite in presence of calcium source. The calcite precipitate in the pore space of soil sample, where they can move and find oxygen for their activity, at particle-to-particle contact. However, the pore space of soil varied with the type of soil. The pore space in coarse-grained soil are greater than pore space in fine-grained-soil. Therefore, the application of MICP in fine-grained soil is limited. The limitation of MICP application in fine-grained soil was studied in this research with Taipei silty soil. MICP method was successively applied on Taipei silty using two method, namely mixing method and injection method. The unconfined compressive strength test and the electronic cone penetrometer test was used to indicate the improvement of soil shear strength for mixing method and injection method, respectively. An increase of two fold of the shear strength of Taipei silty clay from both method was achieved. Prior to the mixing method and the injection method, the ability of Sporosarcina pasteurii, used in this study, was investigated. High concentration of S. pasteurii inside growing medium promotes high urease activity.
    Soil improvement by natural (coir) fiber was also studied in this research. It shows the strength of the soil increases with the addition of fiber up to 1%. However, MICP gives better result on soil improvement compare to natural fiber. A combination of the two method was attempted. However, MICP application give better response to soil improvement than the combination of fiber and MICP.


    Microbial Induced Calcite Precipitation (MICP) is a new technique of improving the engineering properties of soil, a multidisciplinary technique that involves biology, chemistry and soil mechanics. This technique involves the hydrolysis of urea by urease bacteria enzyme into carbonate ions and ammonium ions that precipitate in form of calcite in presence of calcium source. The calcite precipitate in the pore space of soil sample, where they can move and find oxygen for their activity, at particle-to-particle contact. However, the pore space of soil varied with the type of soil. The pore space in coarse-grained soil are greater than pore space in fine-grained-soil. Therefore, the application of MICP in fine-grained soil is limited. The limitation of MICP application in fine-grained soil was studied in this research with Taipei silty soil. MICP method was successively applied on Taipei silty using two method, namely mixing method and injection method. The unconfined compressive strength test and the electronic cone penetrometer test was used to indicate the improvement of soil shear strength for mixing method and injection method, respectively. An increase of two fold of the shear strength of Taipei silty clay from both method was achieved. Prior to the mixing method and the injection method, the ability of Sporosarcina pasteurii, used in this study, was investigated. High concentration of S. pasteurii inside growing medium promotes high urease activity.
    Soil improvement by natural (coir) fiber was also studied in this research. It shows the strength of the soil increases with the addition of fiber up to 1%. However, MICP gives better result on soil improvement compare to natural fiber. A combination of the two method was attempted. However, MICP application give better response to soil improvement than the combination of fiber and MICP.

    ABSTRACT I ACKNOWLEDGEMENTS III TABLE OF CONTENTS V LIST OF TABLES X LIST OF FIGURES XI Chapter 1 INTRODUCTION 1 1.1 Background 1 1.2 Research objectives 2 1.3 Thesis structure 2 Chapter 2 LITERATURE REVIEW 4 2.1 Introduction 4 2.2 Overview of Microbial Induced Calcite precipitation (MICP) 5 2.2.1 Bio-Chemical reaction 5 2.2.2 Improvement of engineering properties of soils 6 2.3 Urease activity 8 2.4 Factors affecting MICP with soil 10 2.4.1 Geometric constraints 10 2.4.2 Types of bacteria 11 2.4.3 Calcium source 14 2.4.4 Cementation solution concentration 15 2.4.5 Bacteria concentration 17 2.4.6 Injection method 17 2.4.7 Treatment duration and reaction time 18 2.4.8 pH 20 2.4.9 Temperature and moisture content 21 2.5 Natural fibers as soil reinforcement 22 2.6 Summary 25 Chapter 3 LABORATORY TESTS 27 3.1 Introduction 27 3.2 Test program 27 3.3 Equipment 29 3.3.1 Measurement equipment 29 3.3.2 Sterilization equipment 33 3.3.3 Bacteria growth equipment 36 3.3.4 Chemical product for nutrient solution, cementation solution and agar plate 37 3.3.5 Centrifuge machine 38 3.4 Microbial information and growth 39 3.4.1 Microbial information 39 3.4.2 Bacteria growth procedures 40 3.4.3 Bacteria growing system 41 3.4.4 Bacteria counting method 42 3.4.5 Urea hydrolysis investigation 43 3.5 Basic information and properties of soil 44 3.5.1 Soil origin 44 3.5.2 Modified proctor compaction test 44 3.5.3 Specific gravity and hydrometer test 46 3.5.4 Atterbeg limit test 47 3.5.5 XRD 50 3.5.6 Summary 51 3.6 Test procedures 52 3.6.1 Mixing method 53 3.6.1.1 Mixing method mold 54 3.6.1.2 Compaction method 55 3.6.1.3 Samples preparation 56 3.6.1.4 Unconfined compressive strength test (UCS) 58 3.6.2 Mixing method with fibers 59 3.6.2.1 Sample preparation with only fiber 59 3.6.2.2 Sample preparation with fiber and bacterial/cementation solution 60 3.6.3 Injection method 61 3.6.3.1 Injection method equipment 61 3.6.3.2 Filter paper 63 3.6.3.3 Samples preparation 63 3.6.3.4 Small cone penetrometer test (CPT) 66 Chapter 4 TEST RESULTS 69 4.1 Microbial growth and urea hydrolysis investigation results 69 4.1.1 Correlation between Optical Density (O.D) to number of cell per ml 69 4.1.2 pH variation during urea hydrolysis 70 4.1.3 Electrical conductivity variation during urea hydrolysis 71 4.1.4 Influence of bacteria growth on pH 73 4.1.5 Summary 74 4.2 Mixing method 77 4.2.1 Influence of cementation solution on improvement ratio 77 4.2.2 Influence of bacteria concentration on improvement ratio 80 4.2.3 Influence of curing time on improvement ratio 82 4.3 Mixing method with fibers 86 4.3.1 Influence of fiber content on soil improvement 86 4.3.2 Influence of fiber content on MICP 88 4.3.3 Summary 90 4.4 Injection method 92 4.5 DISCUSSION 100 Chapter 5 CONCLUSION AND RECOMMENDATIONS 104 5.1 Conclusion 104 5.2 Recommendations 105

    Abo-El-Enein, S.A., Ali, A.H., Talkhan, F.N., Abdel-Gawwad, H.A., 2012. Utilization of microbial induced calcite precipitation for sand consolidation and mortar crack remediation. HBRC Journal 8, 185-192.
    Achal, V., Pan, X., Özyurt, N., 2011. Improved strength and durability of fly ash-amended concrete by microbial calcite precipitation. Ecological Engineering 37, 554-559.
    Al Qabany, A., Soga, K., Santamarina, C., 2012. Factors Affecting Efficiency of Microbially Induced Calcite Precipitation. Journal of Geotechnical and Geoenvironmental Engineering 138, 992-1001.
    Al Qabany, A.S., K., 2013. Effect of chemical treatment used in MICP on engineering properties of cemented soils Ge´otechnique 63, 9.
    Barkouki, T.H., Martinez, B.C., Mortensen, B.M., Weathers, T.S., De Jong, J.D., Ginn, T.R., Spycher, N.F., Smith, R.W., Fujita, Y., 2011. Forward and Inverse Bio-Geochemical Modeling of Microbially Induced Calcite Precipitation in Half-Meter Column Experiments. Transport in Porous Media 90, 23-39.
    Bordoloi, S., Hussain, R., Garg, A., Sreedeep, S., Zhou, W.-H., 2017. Infiltration characteristics of natural fiber reinforced soil. Transportation Geotechnics 12, 37-44.
    Canakci, H., Sidik, W., Halil Kilic, I., 2015. Effect of bacterial calcium carbonate precipitation on compressibility and shear strength of organic soil. Soils and Foundations 55, 1211-1221.
    Cheng L., M.A.S., R. Cord-Ruwisch3, M. Addis4, T. Hartanto4, and C. Elms4, 2014. Soil Stabilisation by Microbial-Induced Calcite Precipitation (MICP): Investigation into Some Physical and Environmental Aspects.
    Choi, S.-G., Wang, K., Chu, J., 2016. Properties of biocemented, fiber reinforced sand. Construction and Building Materials 120, 623-629.
    De Muynck, W., De Belie, N., Verstraete, W., 2009a. Microbial carbonate precipitation in construction materials: A review. Ecological Engineering 36, 118-136.
    De Muynck, W., Verbeken, K., De Belie, N., Verstraete, W., 2009b. Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. Ecological Engineering 36, 99-111.
    DeJong, J.T., Fritzges, M.B., Nusslein, K., 2006. Microbially induced cementation to control sand response to undrained shear. Journal of Geotechnical and Geoenvironmental Engineering 132, 1381-1392.
    DeJong, J.T., Mortensen, B.M., Martinez, B.C., Nelson, D.C., 2010. Bio-mediated soil improvement. Ecological Engineering 36, 197-210.
    DeJong, J.T., Soga, K., Banwart, S.A., Whalley, W.R., Ginn, T.R., Nelson, D.C., Mortensen, B.M., Martinez, B.C., Barkouki, T., 2011. Soil engineering in vivo: harnessing natural biogeochemical systems for sustainable, multi-functional engineering solutions. J R Soc Interface 8, 1-15.
    Dhami, N.K., Reddy, M.S., Mukherjee, A., 2016. Significant indicators for biomineralisation in sand of varying grain sizes. Construction and Building Materials 104, 198-207.
    Gowthaman, S., Nakashima, K., Kawasaki, S., 2018. A State-of-the-Art Review on Soil Reinforcement Technology Using Natural Plant Fiber Materials: Past Findings, Present Trends and Future Directions. Materials (Basel) 11.
    Harkes, M.P., van Paassen, L.A., Booster, J.L., Whiffin, V.S., van Loosdrecht, M.C.M., 2010. Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecological Engineering 36, 112-117.
    Hejazi, S.M., Sheikhzadeh, M., Abtahi, S.M., Zadhoush, A., 2012. A simple review of soil reinforcement by using natural and synthetic fibers. Construction and Building Materials 30, 100-116.
    Horikoshi K., M.N., Y. KURONO, AND N. SASHIHA, 1984. Cellulases of an alkalophilic Bacillus strain isolated from soil. The Riken Institute, Wako-shi, Saitama 351, Japan.
    Ismail, M.A., Joer, H. A., Randolph, M. F. & Meritt, A., 2002. Cementation of porous materials using calcite. Ge´otechnique 52, 12.
    Janssen, P.H., 2006. Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes. Appl Environ Microbiol 72, 1719-1728.
    Jawad, F., Zheng, J.-J., 2016. Improving Poorly Graded Fine Sand with Microbial Induced Calcite Precipitation. British Journal of Applied Science & Technology 17, 1-9.
    Keykha, H.A., Huat, B.B.K., Asadi, A., Zareian, M., Kawasaki, S., 2015. Electrokinetic properties of pasteurii and aquimarina bacteria. Environmental Geotechnics 2, 181-188.
    Kucharski E. S., Cord-ruwisch R., Whiffin V., and Al-thawadi S. M., "Microbial Biocementation," United States Patent, 2008.
    Lee, L.M., Ng, W.S., Tan, C.K., Hii, S.L., 2012. Bio-Mediated Soil Improvement under Various Concentrations of Cementation Reagent. Applied Mechanics and Materials 204-208, 326-329.
    Lekha, K.R., 2004. Field instrumentation and monitoring of soil erosion in coir geotextile stabilised slopes—A case study. Geotextiles and Geomembranes 22, 399-413.
    Mahdavia A., Sajedib R.H. and Rassac M., 2017. Investigation of Acid-Neutralizing Property of Bacillus cereus GUF8. Biomacromolecular Journal 3.
    Mitchell, H.M.A., and J. Carlos Santamarina, M.ASCE, 2003. Biological Considerations in Geotechnical Engineering. Journal of Geotechnical and Geoenvironmental Engineering, 131, 12.
    Mortensen, B.M., Haber, M.J., DeJong, J.T., Caslake, L.F., Nelson, D.C., 2011. Effects of environmental factors on microbial induced calcium carbonate precipitation. Journal of Applied Microbiology 111, 338-349.
    Nemati, M., Greene, E.A., Voordouw, G., 2005. Permeability profile modification using bacterially formed calcium carbonate: comparison with enzymic option. Process Biochemistry 40, 925-933.
    Partha Krishnamurthy, 2, JASON B. ZITZER,2 SUHAS H. PHADNIS,1,2 NIMISH B. VAKIL,3 AND BRUCE E. DUNN1,, MARY PARLOW, HARRY L. T. MOBLEY, 1998. Helicobacter pylori Containing Only Cytoplasmic Urease Is Susceptible to Acid. American Society for Microbiology 6, 7.
    Rebata-Landa, v., 2007. MICROBIAL ACTIVITY IN SEDIMENTS: EFFECTS ON SOIL BEHAVIOR. Georgia Institute of Technology, p. 173.
    Salifu, E., MacLachlan, E., Iyer, K.R., Knapp, C.W., Tarantino, A., 2016. Application of microbially induced calcite precipitation in erosion mitigation and stabilisation of sandy soil foreshore slopes: A preliminary investigation. Engineering Geology 201, 96-105.
    Sarda, D., Choonia, H.S., Sarode, D.D., Lele, S.S., 2009. Biocalcification by Bacillus pasteurii urease: a novel application. J Ind Microbiol Biotechnol 36, 1111-1115.
    Shannon Stocks-Fischer, J.K.G., Sookie S. Bang, 1999. Microbiological precipitation of CaCO3. Soil Biology and Biochemistry 31, 9.
    Siddique, R., Chahal, N.K., 2011. Effect of ureolytic bacteria on concrete properties. Construction and Building Materials 25, 3791-3801.
    Soon, N.W., Lee, L.M., Khun, T.C., Ling, H.S., 2014. Factors Affecting Improvement in Engineering Properties of Residual Soil through Microbial-Induced Calcite Precipitation. Journal of Geotechnical and Geoenvironmental Engineering 140, 04014006.
    Stuti Mauryaa, D.A.K.S., Dr. P.K.Jainc, Dr.Rakesh Kumard, 2015. Review on Stabilization of Soil Using Coir Fiber. International Journal of Engineering Research 4, 4.
    Muthu Lakshmi S., Sasikala S., Padmavathi V., Priya S., Saranya V., 2018. Utilization of Coconut Coir Fibre For Improving Subgrade Strength Characteristics Of Clayey Sand. nternational Research Journal of Engineering and Technology (IRJET)
    van Paassen, L.A., 2011. Bio-Mediated Ground Improvement: From Laboratory Experiment to Pilot Applications. 4099-4108.
    van Paassen, L.A., Daza, C.M., Staal, M., Sorokin, D.Y., van der Zon, W., van Loosdrecht, M.C.M., 2010. Potential soil reinforcement by biological denitrification. Ecological Engineering 36, 168-175.
    Whiffin, V.S., 2004. Microbial CaCO3 Precipitation for the production of Biocement. Murdoch University, Western Australia.
    Whiffin, V.S., van Paassen, L.A., Harkes, M.P., 2007. Microbial Carbonate Precipitation as a Soil Improvement Technique. Geomicrobiology Journal 24, 417-423.
    William B. Whitman, D.C.C., and William J. Wiebe, 1998. Prokaryotes: The unseen majority. Proc. Natl. Acad. Sci. USA 95, 6.
    Zhang, Q.Z.L.L.C.L.M.L.F.A.a.H., 2014. Factors Affecting Improvement of Engineering Properties of MICP-Treated Soil Catalyzed by Bacteria and Urease ASCE 26, 10.
    Zhang, Y., Guo, H.X., Cheng, X.H., 2014. Influences of calcium sources on microbially induced carbonate precipitation in porous media. Materials Research Innovations 18, S2-79-S72-84.

    無法下載圖示 全文公開日期 2022/07/09 (校內網路)
    全文公開日期 2024/07/09 (校外網路)
    全文公開日期 2024/07/09 (國家圖書館:臺灣博碩士論文系統)
    QR CODE