螢光猝滅(Fluorescence Quenching)是指當螢光染料吸收特定波長的光源時，會從原本穩定的基態，提升至不穩定的激發態，在激發態的情況下，藉由產生螢光的方式來釋放能量，回到穩定的基態，釋放能量的過程與猝滅劑(Quencher)接觸時，會使能量傳遞給猝滅劑，造成螢光強度的減低。
本研究是利用螢光猝滅的原理製作出氧敏感薄膜並將其應用到光學溶氧感測器上。首先將聚苯乙烯(Polystyrene, PS)與氟苯基鉑卟啉(Platinum Tetrakis Pentrafluoropheny Porphine, PtTFPP)摻合，並進一步使用磷酸三丁酯(Tributyl Phosphate, TBP)與碳黑(Carbon Black, CB)改善透氧率與比表面積，藉此提升靈敏度。奈米碳材分散的製程中，CB是使用非離子型界面活性劑Triton-X-100使其均勻分散在其中，最終可以製作出高靈敏度氧敏感薄膜。
研究結果顯示PS與PtTFPP的重量比在150/1時有最高靈敏度(I0/I100)為11.73，Sterm-Volmer曲線的線性率(R2)為0.9955，當添加TBP為PS的3wt%後靈敏度提升到16.51、R2為0.99339，進一步摻合碳黑靈敏度更是到達19.12、R2為0.9916，證實了添加TBP和CB能有效改善薄膜靈敏度。最後我們成功使用簡單且低成本的方式製作出高靈敏度且準確的氧敏感薄膜，在未來可利用此方法發展出各種高靈敏度感測器，並應用於水質檢測的領域上。

Fluorescence quenching means fluorescent dye absorbs a light source of a specific wavelength, it will rise from an originally stable ground state to an unstable excited state, and in the case of an excited state, emit energy by generating fluorescence, and Returning to stable ground state, the process of releasing energy contacts the quencher, and transfers energy to the quencher, resulting in a decrease in fluorescence intensity.
In this study uses the principle of fluorescence quenching to make oxygen sensitive film and apply it to an optical dissolved oxygen sensor. First, polystyrene (polystyrene, PS) is blended with Platinum Tetrakis Pentrafluoropheny Porphine (Platinum Tetrakis Pentrafluoropheny Porphine, PtTFPP), and further used Tributyl Phosphate (Tributyl Phosphate, TBP) and Carbon Black (Carbon Black, CB) improve oxygen permeability and surface area, thereby increasing the sensitivity of fluorescence. In the process of dispersing nano carbon materials, CB is evenly dispersed by using non-ionic surfactant (Triton-X-100). Finally, a highly sensitive oxygen sensitive film can be produced.
The results show that the weight ratio of PS/PtTFPP has the highest sensitivity (I0/I100) of 11.73 at 150/1, the linearity (R2) at Sterm-Volmer curve is 0.9955, and the sensitivity increased to 16.51 after adding TBP to 3 wt%, and R2 is 0.99339. The sensitivity of further blending CB is 19.12 and R2 is 0.9916. It is confirmed that the addition of TBP and CB can effectively improve the sensitivity of the film and have a good linear relationship. Finally, we succeeded in producing high-sensitivity and accurate fluorescent films using a simple and low-cost method. In the future, we can develop various high-sensitivity sensors and apply them to the field of water quality testing.

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