酵素液與藥物的代謝反應一直都是一項重要的研究，代謝反應會使身
體對外在環境做出適當的反應，反過來說，人們可以藉由代謝反應的結果來
推算人體內的 酵素液與外來藥物產生的反 應結果或是對人體可能產生的副
作用，提早進行預防或是找尋解決方法。 然而，有鑑於酵素液與藥物的代謝
反應實驗需要經過十分繁瑣的流程，因而非常耗時。因此本研究旨在利用光
固化 3D列印技術開發一晶片，在模擬人體內環境溫度的情況下，將 加藥、
酵素液與藥物代謝反應、過濾、引導檢測等步驟於晶片本體上執行，省去了
傳統實驗上的液體移動、人為安裝拆卸管材等流程 ，使的實驗耗時大幅縮短
及增加 方便性 。 本研究利用 光固化 3D列印技術製作晶片 ，使晶片本身擁有
極高強度 ，搭配液相層析質譜儀進行檢測，藉由將在晶片內部進行反應的 代
謝後藥物導入質譜儀檢測， 紀錄其產生的質譜訊號 並 多次 與標準品做比對
並藉由同種藥物多次上機進行晶片穩定性測試 ，證明於晶片內產生的代謝
反應與標準品的反應為相同結果， 在檢化 實驗流程的同時獲得相同的代謝
反應結果。
基於 DNA核酸適體 的 傳感器是檢測特定分子的有力工具。然而，合成
卡西酮作為 一種新型精神活性物質 ，近 年 來 大受歡迎。由於卡西酮的核心 结
構易於合成，濫用者可以通過合成核心結構 來逃避法律制裁。因此，極需開
發一種具有核心結構靶向性和可變 換構象 特性的 DNA核酸適體傳感器，以
供現場使用。在此，我們 針對 合 成卡西酮家族設計了一種 新型的 基於 DNA核酸適體的傳感器。在金基板 上 連接了一個短的互補 DNA 以下簡稱
cDNA）序列，然後設計了帶有尾部和螢光基團 的 核酸適體與 cDNA進行雜
交。當核酸適體 與 cDNA雜交時，設計的尾部螢光基團垂至金基板上，導
致能量轉移。將合成卡西酮導入系統後 核酸適體與 cDNA雜交，將會捕
捉合成卡西酮，從而釋放出螢光訊號 。 因此，在 目標 分析物存在的情况下，
核酸適體的構象變化會引發螢光訊號的改變 。 該系統 在 5 nM 至 20 μM 的
濃度範圍内顯示出線性校正曲線 R2 = 0.993）），檢測極限為 60 pM。值得
注意的是，該檢測系統 可在 2 小時内快速改變訊號，從而實現高效的現場
檢測 。

The metabolic reaction of enzymes and drugs has always been an important study. The metabolic response enables the body to respond appropriately to the external environment, and in turn, the results of the metabolic response can be used to predict the outcome of the reaction between the enzymes and drugs in the body, or the possible side effects on the body, so that early prevention or solutions can be found. However, the metabolic reactions of enzymes and drugs are time-consuming because of the tedious process involved. Therefore, this study aims to develop a chip using light-curing 3D printing technology to perform the steps of drug dosing, metabolic reaction of enzyme solution and drug, filtration, and conductive detection on the chip itself under the simulation of human body's ambient temperature, which eliminates the traditional experimental processes such as liquid movement, human installation and removal of tubing, and greatly reduces the time-consumption of the experiments and increases the convenience.
In this study, we utilized light-curing 3D printing technology to produce chips with extremely high strength, and used liquid chromatography mass spectrometry (LC-MS/MS) to detect the metabolites reacting inside the chips, recorded their mass spectral signals and compared them with the standards several times, and tested the stability of the chips with the same drug several times on board the machine, which proved that the metabolic reactions inside the chips were the same as those of the standards, and that we could obtain the same results of metabolism reactions in the same way as the experimental process.
DNA aptamer-based sensors serve as a robust tool for detecting specific molecules. However, synthetic cathinone, a new psychoactive substance, has boomed significantly recently. Given the ease-synthesis core structure of the cathinone, the abuser can synthesize the core structure to avoid the administration of the law. Thus, a DNA aptamer with core structure targeting and switchable conformation properties aptasensor was urgent to develop for on-site use. Here, a novel DNA aptamer-based sensor is designed for the family of synthetic cathinones. A short complementary DNA (cDNA) sequence was attached to the gold substrate, and subsequently, the aptamer, designed with a tail and a fluorescent group, underwent hybridization with the cDNA. As the aptamer hybridized with the cDNA, the designed tail's conformation prolapsed onto the substrate, resulting in an energy transfer and the absence of a signal. Upon the introduction of synthetic cathinone into the system, the aptamer de-hybrid from the cDNA and target to the synthetic cathinone, leading to the release of the fluorescence signal. Therefore, a conformational change in the DNA aptamer triggers a fluorescence signal alteration in the presence of the target analyte. The system demonstrates a linear calibration curve within the concentration range of 5 nM to 20 μM (R2 = 0.993) and achieves a limit of detection of 60 pM. Notably, the platform facilitates rapid signal change within 2 hours, thereby enabling efficient on-site detection.

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