以往的肝病治療都是透過移植手術來完成，但手術失敗的風險以及供體來源不足等仍是未能克服的缺點，近期，肝細胞移植成為肝臟移植替代方法，可有效用來治療肝臟疾病。但先前研究發現，細胞培養於多孔性支架容易直接掉入底部，造成細胞不易堆疊生長。故本研究主要針對支架進行研究，設計新支架與過去實驗所使用的支架進行比較，並透過染色的方式提升支架成型精度，希望能夠提高細胞活性及增生能力以符合肝細胞移植的需求，並應用於肝組織工程。
主材料為PCL-DA及 PGSA，透過拉伸試驗、親疏水性測試及熱性質分析比較不同交聯密度的性質影響。成型方面加入蘇丹黑染料提高成型精度，在0.04wt%的染色濃度下，可將精度誤差控制在1~2%。綜合性質測試結果後以PGSA60+PCL-DA此組材料利用DLP成型系統製作高分子生醫支架並培養小鼠肝細胞(FL83B)，透過進行粒線體活性測試結果，發現階梯型支架因細胞可貼附表面積較大使細胞活性較好。

Currently, the treatment of liver disease need organ transplantation, but the risk of surgery and the shortage of liver supplement are still the drawbacks which can’t be overcome. Recently, liver cell transplantation has become an alternative way to liver transplantation, can be used to treat liver disease. However, previous study found that cells may easy to fall directly into the bottom when culturing in the porous scaffold so that cells are not easy to stack and grow. This study designs a new scaffold to compare with another scaffold which used in the past experiment and through the way of dyeing to enhance the accuracy of scaffold. Hope to improve cell viability and proliferation.
The main materials are PCL-DA and PGSA. The effects of different crosslinking densities were compared by tensile tests, contact angle tests and thermal properties analysis. Add the Sudan Black to improve the accuracy. At the concentration of 0.04wt%, the accuracy error can be controlled at 1~2%. After the results of the above-mentioned test, PGSA60 + PCL-DA was used to fabricate scaffold and the hepatocytes culture. It was found that the step-type scaffold has better cell viability due to it has more area which the cells could attach.

【1】許愷玶，“運用雷射剝蝕生物可降解高分子誘導細胞分化以促
進組織再生”，國立清華大學化學工程研究所，碩士論文
(2016)
【2】C. Liu, Z. Xia, J. T. Czernuszka, “Design and Development of Three-Dimensional Scaffolds for Tissue Engineering.” Chem Eng Res Des ,Vol85(A7)1051-1064.
【3】C. Becker, G. Jakse, “Stem cells for regeneration of urological structures.” Eur Urol 2007;51:1217-28.
【4】宋信文 梁晃千，“建立人類的身體工房-組織工程”，科學發 展第362期，2003年2月，6-11。
【5】楊婷琪，“組織工程的重要元件-生物分子”，工研院經貿中心生醫組，2002年7月。
【6】廖俊仁，“組織工程用多孔隙骨架材料”，工研院生醫工程中 心，2002年
【7】S.YANG Ph.D, K. F.LEONG, M. S. E., M. S. M. E., Z. DU, Ph.D., and C.K CHUA PhD. “The design of scaffolds for use in tissue engineering. Part I. Traditional factors.”. 2001，679-689
【8】許芳豪，“以快速原型技術研究組織工程支架孔徑大小對細胞成長之影響”，國立臺灣科技大學機械工程研究所，碩士論文(2006)
【9】H. Kweon , M.K. Yoo , I.K. Park , T.H. Kim , H.C. Lee , H.S. Lee , J.S. Oh , T. Akaike , C.S. Cho , “A novel degradable polycaprolactone networks for tissue engineering”Biomaterials 24 (2003) 801–808.
【10】J. Gao, P.Y. Crapo, M.D. Wang, “Macroporous elastomeric scaffolds with extensive micropores for soft tissue engineering”, Tissue Eng (2006).
【11】J. Wang, K.G. Boutin, O. Abdulhadi, L.D. Personnat, T. Shazly, R. Langer, C.L. Channick, J.T. Borenstein, “Fully Biodegradable Airway Stents Using Amino Alcohol-BasedPoly(ester amide) Elastomers”, Advanced healthcare materials (2013)
【12】C. L. E. Nijst, J. P. Bruggeman, J. M. Karp, L. Ferreira, A.
Zumbuehl, C. J. Bettinger, R. Langer,''Synthesis and
characterization of photocurable elastomers from poly(glycerol-
co-sebacate)'',Biomacromolecules (2007)3067-3073.
【13】Q. Z. Chen, A. Bismarck, U. Hansen, S. Junaid, M. Q.Tran,
S. E. Harding, N. N. Ali, A. R. Boccaccini, “Characterisation of a
soft elastomer poly(glycerol sebacate) designed to match the
mechanical properties of myocardial tissue”,Biomaterials
(2008)47-57.
【14】M.W. Davis, J.P. Vacanti. “Toward development of an
implantable tissue-engineered liver.” Biomaterials(1996)365 -
372.
【15】J.W. Allen, S.N. Bhatia. “Engineering liver therapies for the
future.”Tissue Eng(2002)725 -737.
【16】胡泉，李穎，周瑾，王常勇，“肝組織工程的研究進展與展
望”，2011
【17】M. H. Zheng, C. Ye, M. Braddock, Y. P. Chen, “Liver tissue
engineering: promises and prospects of new technology.”
Cytotherapy (2010), 349-60.
【18】T. Saadi, et al., “Cellularized biosynthetic microhydrogel
polymersfor intravascular liver tissue regeneration therapy.”
20(21-22).
【19】J. Kasuya, et al., “Reconstruction of 3D stacked hepatocyte
tissuesusing degradable, microporous poly (d,l-lactide-co-
glycolide)membranes. ” 33(9).
【20】E. A. Vogler, “Structure and reactivity of water at biomaterial
surfaces. Advances in Colloid and Interface Science,” 1998.
74(1–3)69-117.
【21】H.C. Fiegel, et al., “Hepatic tissue engineering: from
transplantation to customized cell-based liver directed therapies
from thelaboratory.”
【22】M. Bockhorn, et al., “VEGF is Important for Early Liver
Regeneration After Partial Hepatectomy.” 138(2).
【23】K.M. Kulig, J. P. Vacanti, “Hepatic tissue engineering.”12(3).
【24】Q. Z. Chen, A. Bismarck, U. Hansen, S. Junaid, M. Q. Tran,
S. E. Harding, N. N. Ali, A. R. Boccaccini, ''Characterisation of a
soft elastomer poly(glycerol sebacate) designed to match the
mechanical properties of myocardial tissue'', Biomaterials (2008)
47-57.
【25】 W. C. Jiang, Y. H. Cheng, M. H. Yen, Y. Chang, V. W. Yang, K.
Lee . “Cryo-chemical decellularization of the whole liver for
mesenchymal stem cells-based functional hepatic tissue
engineering. ” Biomaterials (2014)3607-17.
【26】F. P. Melchels, J. Feijen, D. W. Grijpma. “A review on
stereolithography and its applications in biomedical engineering.”
Biomaterials (2010),6121-30.
【27】Y. Sakai, H. Huang, S. Hanada, T. Niino. “Toward engineering of
vascularized three-dimensional liver tissue equivalents possessing
a clinically significant mass.” Biochem Eng J (2010),348-61.
【28】T. V. Liu, A. A. Chen, L. M. Cho, K. D. Jadin, R. L. Sah, S. L. De,
et al. “Fabrication of 3D hepatic tissues by additive
photopatterning of cellular hydrogels.” FASEB J.
Volume21(2007),790-801.
【29】X. Li, J. He, Y. Liu, Q. Zhao, W. Wu, D. Li, et al “Biomaterial
Scaffolds with Biomimetic Fluidic Channels for Hepatocyte
Culture. ” Journal of Bionic Engineering 2013:57-64.
【30】K. A. Woodrow, M. J. Wood, W. M. Saltzman, “Biodegradable
meshes printed with extracellular matrix proteins support
micropatterned hepatocyte cultures”, Tissue Eng A (2009)
1169–1179.
【31】I. Zein, D. W. Hutmacher, S. H. Teoh, “Fused deposition modeling
of novel scaffold architectures for tissue engineering
applications”, Biomaterials(2002) 1169–1185
【32】Organovo: http://organovo.com/
【33】D. J. Cornelissen, A. Faulkner-Jones, W. Shu,“Current
developments in 3D bioprinting for tissue engineering.”Current Opinion in Biomedical Engineering 2017.
【34】李孟龍，“動態光罩快速原型系統製造組織工程支架之
研發”，國立台灣科技大學機械工程研究所，碩士論文
(2005).
【35】陳俊豪，“光固化快速成型技術製作組織工程支架之研
究”，國立台灣科技大學機械工程研究所，碩士論文(2006).
【36】許貽玨，“光聚合生物可分解材料應用於RP技術製作
組織工程支架性質之研究”，國立台灣科技大學機械工程研
究所，碩士論文(2007).
【37】陳茂揚，“光固化快速成型系統製作3D組織工程支架”
國立台灣科技大學機械工程研究所，碩士論文(2008).
【38】曾俊元，“動態光罩快速成型系統光聚合PCL-PEG-PCL製
作3D組織工程支架”，國立台灣科技大學機械工程研究
所，碩士論文(2008).
【39】薛智仁，“動態光罩快速成型系統製作3D PCL管狀多
孔性組織工程支架之研究”，國立台灣科技大學機械工程研究所，碩士論文(2009).
【40】謝浚雄，“光聚合PCL材料系統成份探討及其應用於快
速成型3D組織工程支架”，國立台灣科技大學機械工程研
究所，碩士論文(2011).
【41】孫凱閔，“PCL結合PEG-acrylate透過動態光罩成型系統製
作3D多孔性組織工程支架”，國立台灣科技大學機械工程
研究所，碩士論文(2011).
【42】侯佳延，“PCL結合PEG-diacrylate透過反射式動態光罩成型系統製作3D多孔性組織工程支架”，國立台灣科技大學機械工程研究所，碩士論文(2012).
【43】楊淯凱，“以積層製造技術光固化PCL-PEG-diacrylate之材
料性質與組織工程支架成型性探討”，國立台灣科技大學機
械工程研究所，碩士論文(2013).
【44】魏廷宇，“以材料染色改善光固化PCL-DA/PEG-DA支架精度之研究”，國立台灣科技大學機械工程研究所，碩士論文(2015).
【45】許淯維，“使用積層製造技術製作光固化PCL-DA+PEG-
DA/PGSA支架應用於肝組織工程之研究”，國立台灣科技大
學機械工程研究所，碩士論文(2016)
【46】Texas Instrument-About DLP® technology
http://www.dlp.com/tw/
【47】acer-H6510BD specification
https://www.acer.com/ac/en/AU/content/model/MR.JFZ11.00E
【48】原子世界-蓮花效應的原理
http://www.hk-phy.org/atomic_world/lotus/lotus02_c.html
【49】歐陽偉森，“光聚合可吸收高分子PGSA之開發與分析”，國
立清華大學化學工程學系，碩士論文(2015)
【50】J. P. Schneider, L. Pedersen, C. Muhlfeld, M. Ochs. “Staining
histological lung sections with Sudan Black B or Sudan III for
automated identification of alveolar epithelial type II cells,”Acta
histochemica,Volume117(2015),675-80.
【51】SIGMA-ALDRICH
http://www.sigmaaldrich.com/catalog/substance/sudanblackb4565
4419725511?lang=en®ion=TW&attrlist=Brand