含有奈米銀之PVDF/PAN電紡膜接枝明膠之探討｜國立臺灣科技大學博碩士論文系統

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研究生：	程柏達 Bo-da Cheng
論文名稱：	含有奈米銀之PVDF/PAN電紡膜接枝明膠之探討 A Study on Immobilization of Gelatin onto PVDF/PAN Electrospun Membrane Containing Silver Nanoparticles
指導教授：	蘇清淵 Ching-iuan Su
口試委員:	王英靖 Ying-jing Wang 楊銘乾 Ming-chien Yang 鄭國彬 Kuo-bin Cheng
學位類別：	碩士 Master
系所名稱：	工程學院 - 材料科學與工程系 Department of Materials Science and Engineering
論文出版年：	2008
畢業學年度：	96
語文別：	中文
論文頁數：	68
中文關鍵詞：	靜電紡絲、聚偏二氟乙烯、明膠、銀、生物相容性、抗菌
外文關鍵詞：	Electrospinning, Polyvinylidene fluoride(PVDF), Gelatin, Silver, Biocompatibility, antimicrobial
相關次數：	點閱：291 下載：6
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上一筆

本實驗主要藉由混合二種不同材料來克服靜電紡絲膜後加工困難的缺點，第一部份由於純PAN在經由NaOH處理後，電紡膜3D結構會大量消失，所以混摻PVDF進行電紡，接著使用SEM探討PVDF/PAN在濃度2.5wt%、5wt%、7.5wt%、10wt%；電壓10kV、15kV、20kV；工作距離6cm、11m、16cm何者為最佳化參數條件後，再探討不同含量AgNO3對纖維直徑的變化，第二部份PVDF/PAN/Ag電紡膜擔任基材的角色，在基材上利用NaOH及HCl進行羧酸基改質後，加入 EDC當官能基活化劑，使Gelatin與PAN-COOH經由醯胺化反應形成PAN-Gelatin，第三部份將已接枝上Gelatin的PVDF/PAN/Ag電紡膜進行表面接枝量、生物相容性及血液相容性的測定。
實驗結果顯示PVDF/PAN/Ag電紡膜在濃度5wt%、電壓20kV、工作距離11cm、針頭GT23和相對比例為1:4時，纖維直徑有最佳的表現81±_8^17nm，FTIR測定出電紡膜有N-H基的存在，APTT及PT顯示電紡膜接枝明膠血液相容性良好，無助凝血及抗凝血的表現，血小板吸附量有上升的趨勢，L929纖維母細胞生長良好，抗菌性方面，奈米銀在長時間實驗中對明膠有了保護的作用。

The paper is researching into how to overcome the defect of electrospinning membrane was difficult to post-production processed. Section 1 investigated the morphology and optimal parameters of PAN/PVDF electrospinning membrane. The processing parameters including voltage, concentration, ratio of materials, distance between needle and collector. The membrane were prepared by electrospinning PAN/PVDF solutions containing different amounts of AgNO3 in DMF solution when we found the optimal parameters out. The average diameter of the pure PAN/PVDF nanofibers was 154±_█(21)^31 and decreased with increasing of AgNO3 was 81±_8^17nm. Section 2 the PAN/PVDF/Ag electrospinning membrane was hydrolyzed and grafted with gelatin via esterification with EDC. Section 3 the resulting gelatin-immobilizing membranes were characterized with Fourier transform infrared spectrometer(FTIR). Investigated the hemocompatibility and biocompatibility of PAN membrane surface-immobilized with gelatin. Result revealed that Gelatin-immobilizing has stable hemocompatibility and improve biocompatibility and platelet aggregation. In long time test, the effects of silver on the antimicrobial were shown.

目錄
中文摘要                                                 ii
英文摘要                                                 iii
誌謝                                                     iv
目錄                                                      v
圖目錄　　　　　　　　　　    　　　　　　　　　　  　　viii
表目錄                                                   xi
第一章	緒論                                               1
1-1前言                                                 1
1-2研究背景與目的                                       3
第二章 文獻回顧與理論基礎                                 5
2-1靜電紡絲                                             5
2-1-1靜電紡絲原理                                     5
2-1-2靜電紡絲裝置                                     6
2-1-3靜電紡絲主要參數                                 7
2-1-4高揮發性溶液製程阻塞與解決                       8
2-2材料簡介                                             9
2-2-1聚偏二氟乙烯(PVDF)                               9
2-2-2聚丙烯(PAN)                                    9
2-2-3明膠(Gelatin)                                      9
2-2-4 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide(EDC)   10
2-3高分子材料細菌黏著性                                11
2-4殺菌機制                                            12
2-5奈米銀還原                                          13
2-6血小板與凝血作用                                    14
2-6-1血小板構造                                      14
2-6-2血小板止血機制                                  14
2-7血液的凝固機制(Blood coagulation)                      16
2-8高分子生醫材料                                      19
2-9表面改質                                            20
第三章 實驗材料與方法                                    23
3-1實驗儀器                                            23
3-2實驗藥品                                            24
3-3實驗流程                                            25
3-4奈米銀製備                                          26
3-4-1 AgNO3還原成奈米銀                              26
3-4-2奈米銀粒徑評估                                  26
3-5薄膜製備                                            27
3-5-1 PVDF/PAN薄膜製備                              27
3-5-2產量評估                                        28
3-5-3 PVDF/PAN添加奈米銀薄膜製備                    28
3-6表面改質                                            28
3-7掃描式電子顯微鏡及能量散譜分析(SEM&EDS)           30
3-8表面接枝密度                                        31
3-8-1羧酸基(carboxylic acid)接枝量的染色檢驗             31
3-8-2胺基(amino)接枝量的染色檢驗                      31
3-9接觸角測試                                          32
3-10血液相容性                                         32
3-10-1活化部份凝血活時間(APTT)                     32
3-10-2血小板附著試驗                                 33
3-11生物相容性                                         34
3-11-1細胞培養                                       34
3-11-2細胞增生性                                     35
3-12抗菌性測定                                         37
第四章 結果與討論                                        38
4-1奈米銀粒徑評估                                      38
4-2 PVDF/PAN薄膜最佳工作條件                          39
4-3 PVDF/PAN不同比例對產量之影響                      46
4-4添加奈米銀之PVDF/PAN電紡膜探討                    47
4-5奈米銀於P/P28電紡膜中分佈情形                       48
4-6 P/P28改質後微觀現象                                 49
4-7 FTIR測定官能基                                     50
4-8表面接枝量密度                                      51
4-9接觸角測試                                          54
4-10血液相容性                                         55
4-10-1血小板吸附量                                   55
4-10-2部份活化凝血時間及凝血原時間(APTT&PT)       56
4-11抗菌性評估                                         58
4-11-1無添加奈米銀之樣品                             58
4-11-2添加奈米銀之樣品                               60
4-12生物相容性                                         61
第五章 結論                                              62
參考文獻                                                 63
                                

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