Surface plasmon resonance (SPR) has been developed rapidly in many sensing applications, such as gas sensing and solution concentration detection. SPR sensor principle is based on the interaction between the waveguide, thin metal, and analyte material, which can be observed from the reflective response curve. SPR sensor characterization provides a label-free and real-time detection with high sensitive response against detected material. In this thesis, the wavelength modulated SPR sensor was investigated in both numerical simulation and experiments.
Matlab commercial software was utilized to simulate Fresnel equations for the prism constructed SPR sensor. The calculation confirmed that the gold and silver metals were the best selection in sensor applications in telecommunication wavelength range, since they had a highest spectral sensitivity response and were represented by narrow and deep reflection response curve shapes. Reflection response at visible and telecommunication wavelength theoretically demonstrated and confirmed that the telecommunication wavelength range provided smaller full width at half Maximum (FWHM) profiles, which represented higher spectral sensitivity than visible wavelength. Another simulation also conducted the wavelength modulation at different incidence angles. Different incident angles will shift the resonance curve in wavelength modulation. The tuning wavelength between 1510 and 1590 nm theoretically showed that the highest linearity and spectral sensitivity in the refractive index range of 1.3119 to 1.31635 could be obtained in the incidence angle of 62.6o.
The SPR sensor was also utilized to measure the time response of glucose oxidation reaction with Glucose Oxidase (GOD) catalyst in a fixed wavelength of 1550 nm and incidence angle of 62.6o. The sensing performance as time response could be improved significantly by applying moving average calculation to remove noise in the output power. The SPR sensor is capable to measure time response of 0.25% glucose solutions and owns the potential in blood glucose sensing in the human body. The time response measurement in the current research exhibits a linear response with maximum sensitivity of 0.16x10-3 dBm/mM or equal to 1 mWatt/mM. The fastest response of designed sensor with fixed angle and fixed wavelength achieve 12.34831 mM/s.

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