本研究為設計一具電場響應之生物相容性藥物載體模型，透過交流感應電場誘導帶電奈米粒子之電泳行為，進而加速藥物之釋放。利用生物可降解之高分子聚己內酯(Polycaprolactone diol，PCL diol)為基材，經末端官能基改質為聚己內酯(Polycaprolactone diacrylate，PCLDA)，由IR、NMR證實官能基成功改質。以乳化聚合法將聚己內酯(PCLDA)合成聚己內酯奈米粒子，並透過尼羅紅(Nile red)對其進行螢光標記，並利用Zeta-sizer及DLS探討不同參數下的粒徑與電位變化。由UV-vis測量得在尼羅紅200ug/ml的濃度下具有71%的藥物包覆率，並且在一個月內僅損失10%的藥物量。藉由CLSM觀察螢光包覆影像。對聚己內酯奈米粒子施加不同頻率及電壓之交流電場，利用OM觀察微觀下之變化，並藉由雷射去探討聚己內酯奈米粒子受電場響應之穿透率，其中以6V、6mHz之交流電穿透率最高。由UV-Vis觀察聚己內酯/尼羅紅奈米粒子在上述特定交流電場下之釋放率，在30分鐘釋放率可達30%。
將聚己內酯/尼羅紅奈米粒子與RAW264.7細胞共培養，再利用聚己內酯粒子之電響應特性，對其施加6V、6mHz交流電，利用CLSM觀察細胞攝取粒子情況與藥物釋放效果。本研究成功製備出具電場響應之聚己內酯奈米粒子模型，能透過交流電場誘導粒子泳動加速藥物之釋放。藉由調控粒徑與表面官能化修飾，電響應藥物遞送系統可以克服常規藥物製劑的缺點，它們能夠在特定的部位和時間以受控的方式遞送藥物，未來於癌症治療具有相當之潛力。

In this study, we prepared a biocompatible drug carrier model with electric-field response. First, we modified polycaprolactone diol’s terminal functional group to polycaprolactone diacrylate(PCLDA), which has been comfirmed by IR and NMR spectrum. Second, we used emulsion polymerization to synthesize the polycaprolactone nanoparticles with PCLDA and also labled with nile red via drug encapsulation. Using Zeta-sizer and DLS to explore particle size and potential changes under different concentration of SDS.Furthermore, a high loading efficiency 71% and high stability carrier that only loss approximately 10% drug amounts during one month nanocarrier was prepared, which is measured by UV-vis spectrum, and then we observed nanoparticles containing red fluorescence images by CLSM. Applying an alternating electric-field of different frequencies and voltages to the polycaprolactone nanoparticles, observing the change of nanoparticles under the electric-field by OM, and exploring the penetration rate of the polycaprolactone nanoparticles under the electric-field by laser, we found that the penetrationg rate is the highest under specific alternating electric-field, which is 6 volts and 6 mHz. The release rate reached 30% in 30 minutes under the alternating electric-field(6V、6mHz) was observed by UV-vis spectrum.
The polycaprolactone/Nile red nanoparticles were co-cultured with RAW264.7 cells. Owing to the characteristic of electric-field response of the polycaprolactone nanoparticles, we applied an alternating electric-field with 6 volts and 6 mHz, then using CLSM to observe the cellular uptake and drug release.
In this study, a polycaprolactone nanoparticle model with electric field response was successfully prepared. By controlling particle size and particle’s surface functionalization, the electric-field responsive drug delivery system can overcome the disadvantages of conventional pharmaceutical preparations. They are capable of delivering drugs in a controlled manner at specific sites and times, and have considerable potential for cancer treatments in the future.

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