本研究運用再結晶法來製備聚己內酯/海藻酸(PCL/ALG)摻合支架，利用ALG無定形態的高分子鏈混摻插排到PCL-diol的半結晶態高分子中，並進行冷凍乾燥來固定形貌，再利用鈣交聯反應製備出三維多孔隙支架。而我們將探討PCL/ALG摻合支架不同比例的形貌，並透過傅立葉轉換紅外線光譜儀(FT-IR)探討兩種高分子摻合狀態與ALG氫鍵轉移至PCL的變化。再從熱性質分析PCL在經過摻合後的相對結晶度下降32.5%，進而證實摻合支架的良好分散性與互溶性，而支架中高分子分散性的差異將改善PCL/ALG摻合支架的機械性質與降解率，使摻合支架提升30%的機械強度與25%的延展性，同時改善原本ALG及PCL過低的降解率特性，將降解率提高到17.2%。
而為了進一步了解PCL/ALG摻合支架在骨組織工程的應用，我們將摻合支架進行人類成骨肉瘤MG-63細胞培養後的生物活性分析、成骨礦化分析及細胞在支架上的形貌與OCN、OPN蛋白質表徵。藉由這些分析驗證PCL/ALG摻合支架對於骨細胞的增殖與分化有明顯的幫助。最後，我們執行週期性動態培養測試，讓細胞在生長時接受外在力量刺激，並利用摻合支架的機械穩定性讓整體支架所受的應力相同，使應變均勻地對組織之結構、形態和功能產生影響，使其在提升細胞活性及分化能力表現更為顯著。

In this study, we designed and fabricated the polycaprolactone / alginic acid (PCL/ALG) blended scaffolds by recrystallization method. ALG amorphous polymers were mixed dislocated PCL-diol semi-crystalline polymers. The PCL/ALG blended scaffolds freeze-dried and crosslinked with calcium ions (Ca2+) to form the PCL /ALG blended scaffolds.
The morphology of PCL/ALG blended scaffolds in different proportions can be oberserved by Scanning electron microscope (SEM). The change in hydrogen bond transfer and compatibility of the two polymers by Fourier transform infrared spectroscopy (FT-IR). The analysis on thermal behaviors of PCL/ALG blended scaffolds is addressed to study. Because of low degree of relative crystallinity, it shows the good dispersion and miscibility of the blended scaffold. Furthmore, the difference in dispersion will improve the mechanical properties and degradation rate of PCL/ALG blended scaffolds.
The growth behavior of MG-63 cells on the PCL / ALG blended scaffolds can be confirmed by biological activity analysis, osteogenic mineralization analysis, analysis of OCN、OPN protein characterization and cell morphology on the scaffold. In these results, the PCL/ALG blended scaffold promotes both cell proliferation and differentiation, and the morphology of cell aggregation and growth can also be clearly observed under the microscope. Lastly, we applied cyclic mechanical stretching to MG-63 cells on blended scaffolds and investigate the growth behavior of MG-63 cells. Because of the CS-P2A1 blended scaffold has good mechanical properties to make the stress distribute evenly, it shows more significant in enhancing cell activity and differentiation ability.

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