藍藻蛋白又稱藻青素，它是一種非核醣體合成的蛋白質。一百多年前由Borzi觀察藍綠藻所發現，其外觀為顆粒狀，藍藻蛋白的組成為等莫耳比的arginine與aspartic acid，以aspartic acid為骨架，以精胺酸的α-胺基與天門冬胺酸的β-羧基進行縮和反應。
本實驗利用含Synechocystis sp. PCC6803的藍藻蛋白合成酶基因cphA，接上載體送入大腸桿菌所產的藍藻蛋白，利用所產的藍藻蛋白進行其物理化學分析、生物相容性的測試。其物理化學分析利用FTIR得知官能基，SEM觀察蛋白質與glutaraldehyde交聯後的表面情形，以及測試蛋白質交聯後是否會影響蛋白質吸附，並利用TGA及DSC測試水溶性與非水溶性藍藻蛋白的裂解點和熔點，觀察其熱穩定度。
由物理測試實驗所得，FTIR所得數據中顯示水溶與非水溶藍藻蛋白都有胺基的官能基。但lysine上的C-N鍵呈現不同的吸收。蛋白質吸附實驗顯示glutaraldehyde交聯後並不會對蛋白質產生吸附。交聯後所得的膜在SEM觀測下4 %、6 %非水溶性藍藻蛋白膜有絲狀及片狀情況，2 %非水溶性藍藻蛋白呈現顆粒平滑狀況。TGA及DSC所測得在250 0C為可溶與不可溶裂解點，然而不可溶熔點大約
170 oC~180 oC。
本實驗利用Raw 264.7細胞對於非水溶性藍藻蛋白與glutaraldehyde交聯的薄膜以及水溶性藍藻蛋白薄膜的免疫反應測試，CHO cell 及Raw 264.7在非水溶性與水溶性藍藻蛋白分別與glutaraldehyde交聯後之薄膜上生長情況。由實驗顯示藍藻蛋白對Raw 264.7不會產生免疫反應，但細胞在高濃度下生長情況不太好，且細胞在交聯膜上會產生形態會變異。
綜合以上藍藻蛋白含豐富的胺基以及熱穩定性對免疫細胞不會產生影響，只會影響到細胞貼附。

Borzi found the cyanophycin by the observation of blue-green algae. Its appearance is granular and it is composed of the equimolar ratio aspartic acid and arginine. The backbone of cyanophycin is aspartic acid, and the side chain structure is composed arginine.
In this study, we used E.coli which include Synechocystis sp. PCC6803 cyanobacteria protein synthase cphA to produce cyanophycin. Then the produced cyanophycin were used for physical and biocompatibility assays. We used FTIR to analyze the functional groups of cyanophycin, SEM to observe the surface which is cross linked by glutaraldehyde and cyanophycin, TGA and DSC to detect the thermal stability and melting point, and assay the protein adsorption.
The results showed of protein adsorption experiment confirm no protein adsorption. The melting and recomposition points of soluble and insoluble cyanophycin are about 170~185 oC and 250 oC, respectively.
In biocompatibility, we test the biological immune response and cell adhesion. The Raw 264.7 culture on the surface of insoluble cyanophycin cross-linked with glutaraldehyde and of soluble cyanophycin medium, and compare the toxicity with LPS stimulus. The final result, the insoluble cyanophycin cross-linked and sluble cyanophycin didn’t provoke the immune response. For CHO cell on the insoluble cross-linked surface and high concentration medium, the relative grow velocity is reduce, but for Raw 264.7 is near control.
Summary, cyanophycin did’t provoke immune response, has good thermal stability, contain lots of amino group which can react easily for modification the characterization.

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