本研究以靜電紡絲法製備左旋聚乳酸(PLLA)電紡纖維膜，再以氧電漿改質纖維表面，接枝上3-胺丙基三乙氧基矽烷(APTES)，接著將此薄膜浸於聚麩胺酸(γ-PGA)水溶液中使其表面固定一層g-PGA，使PLLA 薄膜由疏水性轉變成高親水性。透過物性分析、材料穩定性測試、生物相容性分析，評估其對於應用於傷口敷材之可行性。
在靜電紡絲製成參數條件部分，針對PLLA濃度、工作電壓與流速進行深入探討，並統計分析其纖維直徑，結果證實靜電紡絲之高比表面積、高孔隙度特性。而經由體外細胞相容性測試，顯示g-PGA改質之PLLA無細胞毒性，且纖維母細胞在其表面的增生性高於PLLA。所以藉由g-PGA與PLLA兩種材料互相配合，可擴大聚麩胺酸在創傷敷材之應用性。

In this study, poly(L-lactic acid) (PLLA) non-woven membrane was prepared by electrospinning technique, followed by surface modification with oxygen plasma and grafting of 3-aminopropyl triethoxysilane (APTES), then immersed in poly(g-glutamic acid) (g-PGA) solution to form on the surface a layer of g-PGA. In so doing, hydrophobic PLLA would become highly hydrophilic. Through characterization of physical properties, stability testing, and biocompatibility, the feasibility of these layered mats for wound dressing was evaluated.
The electrospinning parameters for PLLA, including concentration, voltage, and flow rate, were investigated. Statistical analysis of the fiber diameter was performed to confirm the high specific surface area and high porosity. In vitro cytocompatibility tests show that g-PGA modified PLLA was non-cytotoxic and exhibited higher proliferation for fibroblasts than on PLLA. Thus the surface modification of g-PGA will improve the applicability of PLLA as a material for wound dressing.

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