為了製備出高靈敏度與低偵測極限的非酵素型生物感測器，本研究利用微波電漿系統，以快速且無需要溶劑的方式，製備銅氧化物電極，以感測不同的活性物質。在應用方面，將銅氧化物電極結合 poly(HEMA-co-SBMA) 水膠，探討應用在可穿戴式感測器的可行性。
本研究分為三個部分，第一部分探討銅氧化物電極性質，由掃描式電子顯微鏡 (SEM) 觀察銅氧化物的表面型態，X 光繞射儀 (XRD) 與化學分析電子能譜儀 (ESCA) 確認銅氧化物的組成，三用電表及循環伏安法分析導電度與電活性面積。第二部分為銅氧化物電極感測不同活性物質，將電漿與熱處理電極進行比較，以葡萄糖進行感測，MP120/0.8Cu 有最好的感測表現，具有低偵測極限 (1.25 μM)、線性範圍 (0.1 – 6 mM) 及高靈敏度 (2.04 mA/mM cm2)。以雙氧水進行感測，MP120/1.5Cu 有最好的感測表現，具有低偵測極限 (25.67 μM)、線性範圍 (0.1 - 5 mM) 及高靈敏度 (0.86 mA/mM cm2)。以抗壞血酸進行感測，MP120/0.8Cu 有最好的感測表現，具有低偵測極限 (35.78 μM)、線性範圍 (0.1 - 7 mM) 及高靈敏度 (1.66 mA/mM cm2)。第三部分為探討銅氧化物電極包覆 poly(HEMA-co-SBMA) 水膠對感測之影響，以葡萄糖與雙氧水感測作為比較，實驗結果顯示，包覆 poly(HEMA-co-SBMA) 水膠之電極所計算出的靈敏度與偵測極限並無差異，說明包覆水膠不會對感測表現造成影響。

This thesis aims to prepare non-enzymatic biosensor with high sensitivity and low limit of detection (LOD) by applying the derivative of copper oxides. This study used microwave plasma to prepare the copper oxide electrode which shows the advantages of rapid and solvent-free processing. Then, the copper oxide electrodes were used to detect different electroactive species. For the furthering application, the copper oxide electrodes were covered by poly(HEMA-co-SBMA) hydrogels for the potential to combine the hydrogels with metal oxide.
This thesis is composed of three parts. The first part focused on the characterization of copper oxide electrode. Scanning electron microscope (SEM) was used to observe surface morphology. X-ray diffractometer (XRD) and electron spectroscopy for chemical analysis (ESCA) were used to analyze the chemical state of copper oxide. Digital multimeter was selected to measure the resistance. The electroactive area was calculated by cyclic voltammetry. In the second part, the copper oxide electrodes were applied to detect different electroactive species. The experiments were conducted by the copper oxide electrodes which were treated by microwave plasma or heat. For glucose detection, MP120/0.8Cu showed the best performance with the lowest LOD (1.25 μM), the highest sensitivity (2.04 mA/mM cm2) and a linear range for 0.1 mM to 6 mM. For H2O2 detection, MP120/1.5Cu revealed the best performance with the lowest LOD (25.67 μM), the highest sensitivity (0.86 mA/mM cm2) and a linear range for 0.1 mM to 5 mM. For ascorbic acid detection, MP120/0.8Cu exhibited the best performance with the lowest LOD (35.78 μM), the highest sensitivity (1.66mA/mM cm2) and a linear range for 0.1 mM to 7 mM. Finally, the effect of copper oxide electrode covered by poly(HEMA-co-SBMA) hydrogel for sensing was investigated. To compare the performance between glucose and hydrogen peroxide sensing, the experimental results showed that the calculated sensitivity and LOD of the copper oxide electrode covered with poly(HEMA-co-SBMA) hydrogel showed no difference, indicating that the incorporation of hydrogel can be further used to facilitate the potential flexible electrode.
In conclusion, copper oxide electrodes were successfully prepared by microwave plasma system in this study and can be applied to detect glucose, hydrogen peroxide and ascorbic acid with good sensitivity, linear range, and LOD.

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