骨誘導性（Osteoinduction）為材料所需具備的一項重要的性質，在本研究中利用模擬人體體液（Simulated body fluid，SBF）來披覆仿生礦化的氫氧基磷灰石於幾丁聚醣/動物明膠支架上，期望利用此仿生礦化層來引導骨細胞生長並誘導其分化以形成新生骨。使用不同長短鏈交聯劑如：三聚磷酸鈉、戊二醛和乙二醛，交聯後的幾丁聚醣/動物明膠膜材進行仿生礦化，並探討氫氧化鈣程序的影響性，XRD、EDS、FTIR、AFM和SEM物性分析結果探討交聯劑在仿生礦化中所造成的差異性。結果顯示，TPP交聯後並浸泡氫氧化鈣7天和SBF14天的膜材有最佳氫氧基磷灰石沉積於表面，TPP交聯劑本身提供更加充足的磷酸根離子，且在礦化程序中更能誘導氫氧基磷灰石生成，使其鈣磷比和結晶性相近於人體骨骼ECM。
將骨類細胞培養於仿生礦化後的膜材，藉此探討各個膜材的生物相容性及骨誘導性，體外細胞實驗細胞增生、細胞活性、鹼性磷酸酶的結果顯示，使用TPP交聯後並且浸泡氫氧化鈣和SBF處理後的膜材皆有較高的效果呈現，能促進較佳細胞行為、細胞生物相容性及骨誘導性。因此在未來有關生醫產業的骨再生材料特性設計和選擇上，本研究將會是值得參考的一大重點，以期能更有效的促進骨再生。

Osteoinduction is a key issue in bone tissue engineering. In this study, the chitosan substrates was modified with the simulated body fluid（SBF）which would create a biomimetic layer on the interface between tissues and scaffolds for the bone formation. In the formation of hydroxyapatitie (HA) crystals by SBF treatment, the chitosan plays an important role as a template for the resulted biomimetic HA layer. The properties of chitosan templates are controlled by different crosslinkers, sodium tripolyphosphate (TPP), glutaraldehyde (GA) and glyoxal, before the SBF immersion. From the experimental results from XRD, EDS, FTIR and SEM analysis, the HA layer on the chitosan substrates crosslinked by TPP has the Ca/P ratio and crystallinity which are much closer to those of natural bones. These results prove that the chitosan membrane is more biomimetic by the crosslinking with TPP. It would be due to that the inter-molecular distances are controlled by these crosslinkers. Comparing with the other corsslinkers used in this research, the molecular size of TPP is more similar to the size of HA crystals in the ECM of human bones. That is to say, the resulted HA crystals are constrained in the chitosan template where the inter-space has be dominated by the TPP. Thus, a highly biomimetic layer is obtained.
To investigate the biocompatibility and osteoinduction effects, the chitosan membranes with SBF treatment are used for the culture of osteoblastic cells in this study. The cellular activity, cell proliferation, cell morphology and alkaline phosphatase (ALPase) expression, were analyzed. The results in this research suggested that the SBF modification would improve the biocompatibility of chitosan substrates. Besides, by the crosslinking with TPP, the SBF-treated shows even higher osteoinduction effects, revealed by the increase in ALPase activity. In conclusion, the SBF process on chitosan membranes can be more efficient in the bone tissue engineering by TPP crosslinking. The excellent properties of biocompatibility and osteoinduction makes the substrates prepared in this study to be with high potential in the promotion of bone regeneration.

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