本研究利用海藻酸(alginate, AL)與幾丁聚醣(chitosan, CS)做為材料，並運用靜電紡絲(electrospinning)製備具有芯殼結構 (core-sheath structure)之海藻酸-幾丁聚醣(AL-CS)之複合纖維，接著運用兩種方式在電紡纖維結構中添加羥基磷灰石(hydroxyapatite, HAp)無機粒子。此外透過帶正電荷特性之幾丁聚醣，與帶負電荷之海藻酸與膠原蛋白 (collagen, Col)結合形成聚電解質(polyelectrolyte)，而製備出具有三層結構之複合電紡纖維 (AL-CS-Col-HAp)及海藻酸-羥基磷灰石-幾丁聚醣-膠原蛋白(AL-HAp-CS-Col)。
我們對電紡溶液進行黏度測試。接著運用掃描式電子顯微鏡(SEM)與穿透式電子顯微鏡(TEM)以及雷射共軛焦顯微鏡(CLSM)對電紡纖維進行多種形態學的觀察。隨後，我們將芯殼結構纖維置入多種微環境下進行材料穩定度的評估，以觀察瞭解纖維的結構損失變化情形。最終，在細胞相容性部分，以骨母細胞(hFOB 1.19)和纖維母細胞(L929)做為細胞模型並進行細胞毒性、細胞增生等測試，用以評估其應用在骨材修復之可行性。
經由實驗結果得知，我們成功製備出海藻酸-幾丁聚醣-膠原蛋白混摻羥基磷灰石 (AL-CS-Col-HAp)之複合式芯殼結構纖維及海藻酸-羥基磷灰石-幾丁聚醣-膠原蛋白(AL-HAp-CS-Col)之複合式芯殼結構纖維。運用掃描式電子顯微鏡與穿透式電子顯微鏡同時觀察到纖維的芯殼結構及HAp粒子大小分佈位置的情形。在穩定度的評估結果顯示，與帶正電荷之幾丁聚醣所形成之聚電解質層，在多種微環境下皆可使膠原蛋白的降解程度大幅降低。體外細胞相容性測試結果，顯示此骨組織支架並無細胞毒性，且細胞貼附量明顯增加。
由以上之實驗結果得知，本實驗製備之電紡纖維可應用於骨組織工程及其相關之研究上。

In this study, fibers with a core-shell structure were prepared via electrospinning alginate (AL) into chitosan (CS) solution. Inorganic hydroxyapatite (HAp) particles were added into the resulting fibrous mat. In addition, a third layer of collagen was coated onto the AL-CS composite fibrous mat to form a three-tier structure.
The viscosity of the electrospinning solution was measured. The morphology of the electrospun mat was examined using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and laser confocal microscopy (CLSM). The stability of the structure of the fibrous mat was assessed in various media. The cytocompatibility was evaluated with osteoblasts and fibroblasts based on cytotoxicity and cell proliferation.
Results from SEM and TEM showed that HAp particles were successfully embedded in the AL-CS-Col composite fibrous mat. Stability assessment showed that the release of collagen from the mat was significantly reduced. Cytocompatibility tests showed that the mat exhibited no cytotoxicity, and cells can proliferate on culturing with the mat.
These results indicate that the AL-CS-Col exhibited positive potential as scaffold for bone tissue engineering.

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