自組裝單分子膜 (self-assembly monolayer, SAM) 為近年來受到廣泛探討及應用之技術。 本研究使用帶 Synechocystis sp.PCC6803 cphA 的 E.coli BL21-CodonPlus(DE3)-RIL 製備的非水溶性藍藻蛋白，分別與聚谷胺酸和玻尿酸自組裝成膜，非水溶性藍藻蛋白含有大量的精胺酸與離胺酸，為聚陽離子；聚谷胺酸與玻尿酸，為聚陰離子。利用靜電力使兩分子相吸，依不同的成膜方式分為兩種，一種為粒子間隨機相互吸附沉降成膜的共沉澱法；另一種為於玻璃基材上，一層接著一層，正負電荷排列的層對層自組裝薄膜。
利用 FT -IR (ATR) 觀察成膜後官能基的變化，使用口測試(Sakaguchi test) 測量非水溶性藍藻蛋白的含量，並以掃描式電子顯微鏡 (SEM) 與原子力顯微鏡 (AFM) 探討其表面型態與粗糙度。將L929 細胞進行初步的細胞生長測試，再將人體的纖維母細胞 Hs68 培養於薄膜，以 MTT assay 測試細胞於薄膜上的生長情形。最後利用L929 做細胞遷移測試，模擬傷口復原程度，來判斷薄膜對細胞遷移能力的影響。
結果顯示藉由比色法與官能基證實，將玻尿酸或聚谷氨酸的製作比例提高，有助於提高薄膜中非水溶性藍藻蛋白含量，而玻尿酸/非水溶性藍藻蛋白製作的共沉澱薄膜與層對層薄膜厚度與粗糙度皆低於聚谷氨酸/非水溶性藍藻蛋白薄膜，比較細胞生長情形與細胞遷移能力以玻尿酸/非水溶性藍藻蛋白薄膜效果最佳，以口試驗換算薄膜中非水溶性藍藻蛋白含量，發現膜中非水溶性藍藻蛋白含量越高，有助於細胞生長。

In recent years, self-assembled monolayer (SAM) technology has
found a wide variety of applications in both nanotechnology and
biomedical fields. In this study , we used two kinds of sources to prepare
self-assembled films. One is the combination of hyaluronic acid and
insoluble cyanophycin, which was obtained by cultivating Escherichia coli
carrying BL21-CodonPlus (DE3)-RIL with Synechocystis sp. PCC6803
cphA. The other one is composed of poly-glutamic acid and insoluble
cyanophycin. Insoluble cyanophycin, containing arginine and lysine, is a
cationic polyelectrolyte, whereas poly-glutamic acid and hyaluronic acid
are anionic polyelectrolytes. Therefore, they can be adsorbed onto each
other due to electrostatic interactions. The film preparation was carried out
by two methods. One was co-precipitation method based on random
adsorption. The other one was layer-by-layer self-assembled method,
which was made of the deposition of alternating charged polyelectrolytes.
The changes in function groups were examined by FTIR (ATR). The
conjugates were analyzed by a colorimetric method, the Sakaguchi test, to
measure the contents of the insoluble cyanophycin. And scanning electron
microscopy (SEM) and atomic force microscopy (AFM) were used to
measure the surface roughness and morphology. The L929 cells and Hs68
fibroblasts were employed to test cell growth. The L929 cells were further
to examine the efficiency of cell migration for a wound recovery.
The results based on the colorimetric method showed that a high
amount of hyaluronic acid or (poly-glutamic acid) was associated with a
high amount of insoluble cyanophycin. The film thickness and roughness
IV
were better for the use of hyaluronic acid and insoluble cyanophycin. For
cell growth and cell migration, (hyaluronic acid)/ (insoluble cyanophycin)
by the co-precipitation method can produce better films compared to the
others. The results of insoluble cyanophycin content analyzed by the
colorimetric method in the films showed that the more cyanophycin
content in the films, the better condition for cell to grow.

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