Bacteriorhodopsin (BR)為Halobacterium salinarium紫色細胞膜(purple membrane，PM)上具有單一方向光驅動質子泵功能之蛋白質，目前已被公認為未來應用於生物光電及全影像技術之最佳生物材料。早期製備一個具有BR定向固定化之生物晶片，其方法是耗時、手續繁雜且效果不彰的。本研究中我們開發出一種既簡單又可以具有高BR光電性質之生物晶片的製備方法。使懸浮於磷酸鹽溶液內的PM附著於導電ITO玻璃表面上，並乾燥後，利用氙燈激發此晶片時會產生高強度的光電訊號。更進一步地針對不同緩衝液及其pH值，以及電沉積法等不同變因對光電流之影響加以探討，且利用XPS分析，我們提出因為磷酸根離子會快速附著於ITO玻璃表面上而有效地促進PM膜的方向性排列，進而增強BR晶片光電流響應之假設。並且測量PM晶片於不同電解液環境下所得到的光電流值，發現若使用添加有10 mM PB的100 mM KCl (pH=7.5)當電解液時，可以讓我們的生物光電晶片產生最高的光電流值，且原生種PM (n-PM)晶片與經過乙醯化修飾PM (A-PM)晶片其光電流訊號發生反向的電解液pH值並不相同。此外，也利用脈衝雷射分別對不同的PM晶片進行激發，發現光源強度和此兩種晶片之光電流值間均呈現兩個不同階段的線性關係。最後，探討添加NaN3或DTT分別對於D96N和G241C 突變PM膜晶片所造成的影響，發現分別添加10 mM NaN3與1 mM DTT於此兩種晶片中，可使晶片的光電流值達到最大。

Bacteriorhodopsin (BR), the unique light-driven proton-pump protein in the purple membrane (PM) of Halobacterium salinarium, has been considered the most promising biomaterial for applications in photoelectrics and holography. However, the former development has been slow so far due to a great demand of orientationally structured BR for producing high-efficiency photoelectric chips. Herein, a simple method to prepare PM-coated chips with promising photoelectric properties was reported. A PM film prepared from a phosphate-buffered solution was casted and dried onto an ITO electrode, yielding high photocurrents while illuminated with a Xenon arc lamp. The studies on effects of buffer compositions and pHs, electrodeposition, and XPS analyses supported the hypothesis that the preferable binding of phosphoric ions onto ITO helped improve the uniformity of PM orientation during the film-casting process. A further study on the electrolyte compositions led to an optimal solution constituted of 10 mM PB and 100 mM KCl, pH 7.5 for the best photocurrent generation. In addition, the photocurrents of the acetylated PM-coated chips reversed at slightly higher pH values in comparison with those of the native-PM coated chips. Two distinct linear relationships between the generated photocurrents and the incident light energy were consistently observed for variously prepared PM chips when they were illuminated with a pulse laser. Finally, for the D96N and G241C mutant PM-coated chips, the addition of 10 mM NaN3 and 1 mM DTT in their respective suspension solutions resulted in achievement of the optimal photocurrent generation.

王世育，”細菌視紫質表面修飾及應用於單層膜光電晶片製備之研究”，國立台灣科技大學化學工程研究所碩士論文(2006)

溫文興，”Bacteriorhodopsin突變蛋白之初步研究”，國立台灣科技大學化學工程研究所碩士論文(2004)

謝竺君，”Bacteriorhodopsin 及其突變蛋白之光電響應”，國立台灣科技大學化學工程研究所碩士論文(2005)

謝謹謙，”Halobacterium halobium紫色細胞膜光電系統的建立”，國立台灣科技大學化學工程研究所碩士論文(2004)

蘇志溫，”Bacteriorhodopsin生物晶片之光電響應”，國立台灣科技大學化學工程研究所碩士論文(2004)

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