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研究生: 曹晉誠
Chin-Cheng Tsao
論文名稱: 漸縮漸擴毛細管應用於對流式PCR的熱流模擬分析
CFD Thermal/Flow Analysis of Converging-Diverging Capillary Applied on Convective PCR
指導教授: 林顯群
Sheam-Chyun Lin
口試委員: 黃緒哲
Shiuh-Jer Huang
周永泰
Yung-Tai Chou
學位類別: 碩士
Master
系所名稱: 工程學院 - 機械工程系
Department of Mechanical Engineering
論文出版年: 2022
畢業學年度: 110
語文別: 中文
論文頁數: 156
中文關鍵詞: 漸縮漸擴毛細管數值模擬溫度控制有效體積
外文關鍵詞: CPCR(Convective polymerase chain reaction), Converging-diverging capillary tube, Annealing volume, Detection time, Point-of-care test (POCT)
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摘 要 I Abstract II 致 謝 IV 目 錄 V 圖索引 IX 表索引 XII 符號索引 XIV 第一章 緒論 1 1.1 前言 1 1.2 文獻回顧 2 1.2.1 聚合酶連鎖反應 2 1.2.2 熱對流式聚合酶連鎖反應(CPCR) 5 1.2.3 數值模擬 13 1.3 研究動機、目的與研究流程 16 第二章 物理模式與理論分析 23 2.1 熱傳基本原理 23 2.1.1 熱傳遞理論 23 2.1.2 熱阻定義 25 2.1.3 自然對流 26 2.2 毛細管設計 27 2.2.1 CPCR之實際運作 27 2.2.2 毛細管尺寸 29 2.2.3 熱源之設計 34 第三章 數值方法 39 3.1 統御方程式 39 3.2 數值計算方法 42 3.2.1 求解流程 43 3.2.2 離散化方程式 45 3.2.3 上風差分法 46 3.2.4 壓力與速度耦合 46 3.3 紊流模組 48 3.4 邊界條件與參數設定 50 第四章 原始CPCR毛細管之熱流場模擬分析 55 4.1 數值模型建立 55 4.1.1 模型架構與元件規格 61 4.1.2 模型設計之限制條件 64 4.2 數值模型與網格規劃 66 4.3 熱流場分析與流場缺失彙整 79 4.3.1 CPCR分析儀之毛細管熱流場分析 79 4.3.2 原始CPCR毛細管之缺失整理 84 第五章 CPCR毛細管之改良方案 86 5.1 粗短型毛細管 86 5.1.1 毛細管之模型設計 87 5.1.2 毛細管之熱流場分析 90 5.1.3 毛細管之性能分析 92 5.2 漸縮漸擴毛細管 94 5.2.1 漸縮漸擴毛細管模型設計 95 5.2.2 漸縮漸擴毛細管之熱流場分析 95 5.2.3 漸縮漸擴毛細管之性能分析 109 第六章 漸縮漸擴毛細管之參數分析與性能優化 110 6.1 不同半徑比之漸縮漸擴管的熱流場分析 110 6.2 熱源和熱沈之溫度調整的PCR特性評估 119 6.2.1 熱源和熱沈的溫度調整後之熱流場分析 121 6.2.2 熱源和熱沈的溫度調整後之溫度控制性分析 130 6.3 不同漸縮漸擴半徑比之毛細管的PCR性能比較 135 第七章 結論與建議 139 7.1 結論 140 7.1.1 對流式PCR運作之必要條件分析 140 7.1.2 不同CPCR毛細管之外型設計 141 7.1.3 漸縮漸擴毛細管之特性分析 145 7.1.4 改善後漸縮漸擴管與原始CPCR毛細管之比較分析 148 7.2 建議 150 參考文獻 155

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