癌症是一種具有複雜異質性結構的危險疾病，所以早期癌症細胞篩檢及診斷是非常重要的。一些標準治療程序，例如活體組織穿刺檢查或手術等皆有可能會提高癌細胞增殖及轉移的程度。金屬玻璃薄膜(Thin film metallic glass, TFMG)因其固有的獨特性質如非晶結構、相對高的疏水性、光滑表面、低磨擦係數、高強度、高韌性、優異的耐腐蝕及耐磨耗等，具有潛力成為一種可以有效防止癌症細胞附著的材料。
本研究利用磁控濺鍍,以醫藥級玻璃和手術刀做為基板沉積相應50nm及200nm厚的Zr53Cu33Al9Ta5金屬玻璃薄膜，另製備了具有相同厚度純鈦薄膜的試片用於對比。本實驗於基板上共培養三種不同的癌細胞，分別為SKGT4(食道癌)、MB231(乳腺癌)、WiDr(結腸癌)，來進行分析評估。對於不同基板上的觀察顯示，各類癌細胞都在鍍有金屬玻璃薄膜的基板上有最小的吸附面積(~0.4-2.6%)與密度(~105-201 cells/mm2)，相對應的最高降低幅度分別達到~86%和~84%。研究的結果顯示，癌細胞在鍍有金屬玻璃薄膜的基板上並沒有發生顯著的增殖與擴散，最重要的原因在於金屬玻璃薄膜擁有低表面能(~29.3mN/m)、優良的疏水性(去離子水的接觸角97.7°)以及降低表面粗糙度(分別將玻璃基板的粗糙度從~0.5降至~0.2 nm 以及將手術刀的粗糙度從~317.7降至~160.4 nm)等優異性質，進而減緩了癌細胞的附著。因此，本研究成功的使用金屬玻璃鍍膜降低了癌細胞的吸附及增殖，同時揭示並探討了癌細胞在已鍍與未鍍金屬玻璃薄膜的玻璃基板上的形態及吸附機制，此外癌細胞於鍍有金屬玻璃的基板之表面形貌僅有微小的板狀偽足及絲狀偽足擴張形成萎縮的細胞狀。

Since cancer is one of dangerous diseases having complex heterogeneous structures, an early diagnosis and cancer screening are critically important. In some cases, standard diagnostic and treatment procedures such as biopsy and surgery could increase the level of proliferation and metastasis of cancer cell. Thin film metallic glass (TFMG) is thought to be one of promising materials to prevent the adhesion of cancer cells due to its unique intrinsic properties such as amorphous structure, relatively high hydrophobicity, smooth surface, small frictions, high strength and toughness, excellent corrosion and abrasion resistance.
Zr53Cu33Al9Ta5 TFMG with different thicknesses of 50 and 200 nm was deposited on substrates of medical-grade glass and scalpel using magnetron sputtering. Pure Ti film was also deposited for comparison. Various types of cancer cells including SKGT4 (esophageal cancer), MB231 (breast cancer), and WiDr (colon cancer) were cultured on the substrates for evaluations. For TFMG-coated glasses, all types of cancer cell are found to have relatively small attachment area (~0.4-3.7%) and low density (~105-261 cells/mm2) among other samples studied. TFMG coating also shows the highest reductions area of cancer cell attachment and density of ~86 and ~84%, respectively. As a result, a cell migration and spreading are found to be not significant in the samples with TFMG coating. The low surface free energy and most hydrophobic nature in TFMG coating with the values of ~29.3 mN/m and 97.7°, respectively, are considered as the foremost factor to inhibit cell attachments. Decreasing glass and scalpel roughness from ~0.5 to 0.2 nm and from ~317.7 to 160.4 nm, respectively, is also important factor to deter cancer cell attachments. Therefore, TFMG coating is shown to successfully minimize the adhesion of cancer cell. In addition, the morphology of cancer cell on TFMG coating shows shrunken-cell morphology with minor spreading of lamellipodia and filopodia.

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