本論文將Pluronic F127混摻carboxymethyl hexanoyl chitosan (CA)，形成一種溫度敏感可注射型水膠，作為封裝細胞的組織工程支架。本論文分成兩個部分來敘述每個注射支架的研發過程。
本研究的第一部分是F127，CA和戊二醛（GA）組成的新型水膠體系，用於包封成纖維細胞（L-929）和人胎兒成骨細胞（hFOB 1.19）。該水膠在水性環境能維持長時間的完整性，以實現較長久的封裝。水膠的熱性質係使用熱重分析（TGA）和示差掃描量熱法（DSC）來測定。在Dulbecco's Modified Eagle's培養基（DMEM）中評估水凝膠的溶脹行為，並通過動態力學分析（DMA）測定機械性能。經由簡單混合包封細胞，並使用alamar blue細胞活力測定法確定包封細胞的活力，並使用螢光成像檢查細胞形態。結果顯示，該系統的Tgel為30℃左右，溶膠 - 凝膠轉化發生在90秒以內。儘管CA和GA的加入稍微降低了剪切模量，但F127/CA/GA凝膠仍能在介質中保持凝膠狀態超過1個月。體外細胞培養研究發現F127/CA/GA水凝膠是非細胞毒性的。此外，包覆的L-929的活性在培養5天后為106%。基於這些結果，這些F127/CA/GA水膠可用於組織工程的包封細胞。
第二部分係由F127，CA和乙二醛（Gx）組成的熱可逆注射水膠上。這種類型的水膠可以使用的時間更長。水膠與Gx交聯，水膠變成凝膠狀態。使用流變儀來研究水膠的化學和機械性質。在本研究中也確定了溶脹比，完整性和凝膠化時間。水膠的凝膠化時間短於80s，凝膠化溫度為25-37℃。水凝膠可以在水性環境中保持凝膠狀態超過1個月。體外毒性試驗也顯示水膠能夠包覆細胞。L-929細胞培養5天後的的細胞活性比初始狀態增加20%。結果顯示，該水膠具有可以被當作細胞包覆之可注射支架。

A thermoresponsive injectable hydrogel based on Pluronic F127 and carboxymethyl hexanoyl chitosan (CA) was developed in this thesis for encapsulating cells as tissue engineering scaffold. This thesis is presented in two parts to describe the development of this injectable scaffold.
The first part of this research is to design a novel hydrogel system composing of F127, CA and glutaraldehyde (GA) for encapsulating fibroblasts (L-929) and human fetal osteoblast (hFOB 1.19). This hydrogel was designed to improve the integrity in the aqueous environment for longer encapsulation. The thermal behavior of the hydrogel was evaluated using Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The swelling behavior of the hydrogel was evaluated in Dulbecco's Modified Eagle's medium (DMEM), and the mechanical properties were determined through dynamic mechanical analysis (DMA). Cells were encapsulated by simple mixing, and the viability of encapsulated cells was determined using alamar blue cell viability assay and the cells morphology was examined using fluorescent imaging. The results indicated that the gelation temperature (Tgel) of this system was around 30°C, when the sol-gel transformation occurred within 90 s. Although the addition of CA and GA reduced the shear moduli slightly, the F127/CA/GA gel was able to remain in gelling state in the medium for more than 1 month. In vitro cell culture study revealed that F127/CA/GA hydrogels were non-cytotoxic. Moreover, the viability of encapsulated L-929 was 106% after incubation for 5 days. Based on these results, these F127/CA/GA hydrogels can be used to encapsulate cells for tissue engineering applications.
The second part is concentrated on thermoreversible injectable hydrogel composing of F127, CA, and glyoxal (Gx). This type of hydrogel can be used for longer time. Being crosslinked with Gx, this hydrogel system exhibited thermoreversibility; it reverted to the liquid state when cooled below Tgel. The mechanical properties of the hydrogel were investigated using rheometer. The swelling ratio, integrity, and gelation time were also determined. The hydrogel exhibited gelation time shorter than 80 s, and the gelation temperature is in the range 25-37C. The hydrogel can maintain the gel state in the aqueous environment for more than 1 month. The in vitro toxicity test also revealed that hydrogel was able to encapsulate cell without toxicity effect. The cell viability of L-929 cells at 5-day incubation was increased 20% higher than the initial state. The results suggested that this hydrogel could be a promising scaffold for cell-encapsulation.

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