本研究以共價鍵結方式將膠原蛋白(collagen, CL)、明膠(gelatin, GL)或多醣類如幾丁聚醣(chitosan, CS)、透明質酸(hyaluronic acid, HA)、硫酸軟骨素(chondroitin-6-sulfate, ChS)等細胞間質固定於多孔性、生物可分解之Biomax非織物，使其成為親水性與細胞相容性之3D生物支架。經接枝後之非織物以ESCA元素分析、拉伸試驗機(tensile tester)和掃瞄式電子顯微鏡(SEM)來確定改質後之材料特性，並測定孔隙度、抗菌性，以及評估其非織物膨潤度和水份擴散及接觸角等非織物之親水性質，同時量測活化部分凝血時間(APTT)、凝血原時間(PT)、血小板凝集程度來評估凝血性質。然後將改質後的膜材作纖維母細胞之體外培養，比較其細胞之增生細胞相容性。本研究結果顯示Biomax高孔隙度非織物經細胞間質固定化後，接枝明膠之織物具有較高之抗張強度，較低之接觸角，較低的血小板貼附量，較長的凝血時間，但無抑菌性。接枝chitosan之織物則有最佳之抑菌性。且接枝CL、GL、CS、HA、ChS之織物對fibroblast細胞增生有助益。

Mechanical and cellular compatible scaffolds for tissue engineering were prepared using the three-dimensional, macroporous Biomax nonwoven fabrics immobilized with extracellular matrices (ECM), such as collagen (CL), gelatin (GL), chitosan (CS), hyaluronic acid (HA), and chondroitin-6-sulfate (ChS). The ECM-immobilizing Biomax nonwoven was characterized by ESCA, tensile tester, and scanning electron microscopy (SEM). The swelling properties were evaluated by water diffusion and contact angle measurement. The hemostatic behavior was observed by the adhesion and aggregation level of platelet. The cellular compatibility was evaluated using 3T3 fibroblasts in vitro culture. The results show that GL-immobilizing Biomax nonwoven fabrics exhibited higher tensile strength, lower contact angle, less platelet adhesion, longer coagulation time, but no bacterial inhibition. CS-immobilizing nonwoven exhibited the highest bacterial inhibition. Nonwoven immobilized with ECM showed improvement in the proliferation of fibroblast.

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