由於目前市售的痤瘡敷料，皆無抗菌及抗發炎之成分添加，對於發炎中的痤瘡，無法有效的抑制其發炎程度，以及細菌之增生，為改善其缺點，因此，本研究利用澆鑄的方式將蠶絲蛋白摻混聚乙烯醇鑄成複合薄膜，做為薄膜之基質(NP-SFPM)，利用氯化鈉製造具有孔洞之薄膜(P-SFPM)，增加其透氣性，並添加具有抗菌及抗發炎功效之茶樹精油，評估其對於治療皮膚痤瘡之功效，經細胞實驗發現，蠶絲蛋白薄膜無細胞毒性，隨著茶樹精油含量的添加，其細胞存活率越低，當茶樹精油添加25微升至NP-SFPM以及P-SFPM時，其細胞存活率分別降低至，67.5 ± 4.0%及49.3±8.2%，選擇其細胞存活率較高之組別，做後續抗發炎以及抑菌之研究，經實驗發現蠶絲蛋白薄膜具有38.6%至44.4%之抗發炎功效，僅需添加微量的(3.125微升、6.25 微升、12.5 微升)茶樹精油，即可抑制LPS誘導巨噬細胞(Raw 264.7)發炎產生NO的濃度，將其應用在抑制痤瘡桿菌(Propionibacterium acnes, P. acnes)增生之實驗，發現蠶絲蛋白薄膜具有78%以上之抑菌功效，且隨著茶樹精油添加至12.5 微升，其抑菌率更可達到98%以上，經由小鼠動物模式驗證，發現從誘發感染後治療一天、三天及九天之組織含菌量變化，實驗組隨者天數的增加，各組之抑菌率更上升28%以上，由此證實本研究所開發出的薄膜，對於皮膚痤瘡的治療有確實之功效。

In this study, we used the way of casting to mix Silk and PVA together to generate a composite membrane, i.e., Non-porous-Silk Fibroin Protein Membranes (NP-SFPM) and subsequently used NaCl to creat pores in the NP-SFPM (Porous-Silk Fibroin Protein Membranes, P-SFPM),for increasing its permeability. We also added Tea Tree Oil (TTO) to abovementioned membranes and then assessed its efficacy on the treatment of skin acne infection.In this study, we found that silk fibroin protein membranes (SFPM) doesn’t have significant cytotoxicity under appropriate amount of TTO addition. When the content of TTO added to NP-SFPM and P-SFPM up to 25 μL , cell viability decreased to, 67.5 ± 4.0% and 49.3 ± 8.2%. Thus, we choose 3.125 μL, 6.25 μL, and 12.5 μL for the following anti-inflammatory and anti-bacterial studies. The experiments prove that the SFPM exhibits a slight anti-inflammatory effect. In addition, under a small amount of extra TTO addition, our membranes show remarkable ability on inhibiting the NO formation in LPS-induced giant macrophages (Raw 264.7). In inhibiting the proliferation of Propionibacterium acnes (P. acnes) experiments, we found that SFPM has mild anti-bacterial effect (78% of bacteriosis), and in the case of 12.5 μL TTO addition, the inhibition ratio can reach approximately 98-99%. In animal model evaluation, we found that increasing the numbers of treatment day, the inhibition rates of P. acnes in the experimental group are significantly increased. Conclusively, we successfully develop a SFP-based membrane, which is potentially useful on the treatment of skin acne problem.

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