有鑒於非病毒載體系統中，使用微脂粒或陽離子型高分子為基因載體，可避免病毒型載體所遭遇到的安全性及免疫性考量。因此，本研究選用親水性高、穩定不會快速分解且生物相容性高的纖維二糖(Cellobiose)及具有疏水性且生物相容性高的12胺基十二烷酸(12-aminododecanoic acid)，鍵結形成一個具有胜肽鍵(Peptide)及親疏水端性質的兩性分子。以此兩性分子作為基因傳遞載體的基礎，期望達到易於被生物所降解並達到傳遞基因的目的。
使用三種不同的材料spermine、spermidine及N-[N'-(tert- butoxy carbonyl)-3-aminopropyl]-N,N-dimethyl-3-aminopropylammonium carbonate (TADAC)分別與N-succinyl -(N'-12-aminododecanoyl)cell- obiosylamido acid (CA-ADA acid)進行合成反應，形成兩性分子。利用濃度為0.5 μg/μl的DNA進行質體核酸膠體電泳測試，測試其是否有包裹DNA的能力。另一方面，調整不同N/P值測試具包裹能力的載體，找出具包裹能力的載體最佳的N/P值。
對於三種兩性分子而言，唯CA-ADA-A-BA包裹DNA效果較為明顯，但是卻不能完全的包裹DNA。然而，有趣的是當作對照組的(N-12-aminododecanoyl) N,N'-bis (3-aminopropyl) butane-1-amino-4- amide (ADA-BA)卻被DNA所包覆著。
本研究發現使用CA-ADA acid為基礎所形成的兩性分子，並不能擁有完全包裹DNA的能力。當去除纖維二糖的部份後，卻能產生較佳包裹DNA的效果，其可能原因可能是纖維二糖對兩性分子所造成的立體障礙所致。因此，未來載體的設計應該朝向將立體障礙減小、兩性分子的電性提高或者將碳鏈加長的方向進行改進，以找出最佳的載體設計。

In non-viral vector system, avoided safety and immunity viral vector of viral vector which used liposomes and cationic polymer to be a vector of gene. Hydrophilic highly, stable and biocompatible hightly cellobiose and hydrophobic and biocompatible hightly 12-aminododecanoic acid were formed an amphipathic polymer. Amphipathic polymer were used as major DNA carrier.
At first, there were three materials for synthesis formed an amphipathic polymer .We used DNA of 0.5 μg/μl to do DNA electrophoresis, and investigated how the ability of the carrier bound DNA. And then, we researched the different N/P ratio of materials, and compared the ability of binding DNA with each other.
As to three kinds of amphipathic polymer, it was obvious that carrier bound DNA for CA-ADA-A-BA. But it could not bind DNA completely. However, it was interesting in ADA-BA.ADA-BA could be bound by DNA.
In this study, we used CA-ADA acid to be formed amphipathic polymer. And then, it could not bind DNA completely. After removing this molecule of cellobiose, it had batter binding effective than CA-ADA-A-BA. In future, we should design higher electric charge of hydrophilic and hydrophilic molecule or longer carbonic chain to achieve optimal design.

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