本實驗以自由基聚合法合成聚甲基丙烯酸磺基甜菜鹼-丙烯酸poly(SBMA-co-AA)之隨機共聚合物(Random copolymers)，並使用孔徑大小 20 m 的尼龍篩網作為基材，接著以物理氣相沉積法(Physical Vapor Deposition)在尼龍篩網表面鍍金形成電極，再以 EDC/NHS 共價鍵固定法將共聚高分子包覆在電極表面上，最後於表面修飾鏈親和素(Streptavidin)與專一性抗體(Anti-EpCAM conjugated Biotin)，結合自製的流體裝置進行大腸癌循環腫瘤細胞的抓取，並利用電化學方法進行定量分析。從實驗結果得知，當 poly(SBMA-co-AA)比例為 1:9 時有較好的抓取率約 52.5 %及抗沾黏效果約 99.8 %，且具有優異的選擇性，而在流速為 1 mL/hr 有最佳的抓取效率。在利用電化學阻抗頻譜分別偵測 5、10、20、40、80顆循環腫瘤細胞，發現阻抗變化有高度的正相關。在加入全血進行仿臨床實驗中，發現此裝置能夠在 1 mL的全血中有效的偵測到 10 顆以上的大腸癌循環腫瘤細胞，且隨著細胞增加同樣也呈現正相關。此外，我們也將電化學方法應用於細胞培養監控，可得知當細胞貼附、生長後，阻抗值隨之上升。本研究成功製備出利用流式篩網裝置專一性抓取大腸癌循環腫瘤細胞於電化學定量分析並能夠監控細胞生長，提供一個快速、便利且高靈敏度的檢測試片。

In this study, we use free radical polymerization to synthesize the random copolymer poly(SBMA-co-AA). First we generate the electrode which is nylon sieve with pore size 20 m and it is plated with gold by physical vapor deposition. Then coat copolymer on the electrode by EDC/NHS reaction and modify the surface by using Streptavidin and its specific antibody (Anti-EpCAM conjugated Biotin). The electrode is combined with handmade fluidic device to capture colorectal cancer circulating tumor cell and analyze the quantity by electrochemical methods. We found that the better capture rate is about 52.5 %, the greater anti-fouling property is approximately 99.8 % and the more excellent selectivity occur when the proportion of poly(SBMA-co-AA) is 1:9. Moreover the best efficiency of capture occurs when the flow rate is at 1 mL/hr. The essay shows that electrochemical impedance spectroscopy is highly positive correlation with circulating tumor cell. And it obtains quite low detection limit for 10 circulating tumor cells in 1 mL whole blood. Additionally we apply electrochemical method in cell monitoring and the results indicate that when the cells adhere and grow, the impedance increases.
We successfully generate a rapid, convenient and high sensitive fluidic device, which can specifically capture colorectal cancer circulating tumor cells, and quantitatively analyzes by electrochemical methods and cell monitoring.

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