本研究使用共沉澱法合成鐵奈米粒子，再將鐵奈米粒子跟TEOS以及APTES反應，在外面包上帶有胺基的二氧化矽殼，並得到大小約為150 nm的奈米粒子，再加入EDC/NHS與Protein G反應形成醯胺鍵(Amide bond)，接著透過Protein G與IgG的生物親和性結合之後，使抗體與抗原反應，達到快速抓取並純化HDL的功能。
本研究成功合成每毫克磁性奈米粒子接枝4.25 μg抗體，並且達到 159.1 μg/100ug HDL之穩定抓取，與先前實驗室所合成之鐵奈米粒子HDL抓取率比較，以APTES 、L-Cystein、Thioglycolic acid作為保護基改質之鐵奈米粒子分別只有 3.5 μg/100uL、12.5 μg/100uL HDL、49.46/ug/100ul之抓取率，本篇使用的氨基改值之二氧化矽磁性核殼奈米粒子的HDL抓取率最佳。
接著將從北醫附醫取得之9名病人純化過的HDL檢體以電化學循環伏安法氧化能力檢測，也與以螢光法測得之氧化數值比較，統計分析得出Pearson's 相關係數為0.98，呈現強相關。
最後將取出plasma的病人血液，加入純化過的HDL，混合均勻後利用奈米粒子重新抓取，並以電化學循環伏安法氧化能力檢測，也也與以螢光法測得之氧化數值比較，統計分析得出Pearson's 相關係數為0.976，也呈現強相關。
對此，藉由免疫沉澱法，快速分離血液中的HDL，並透過電化學循環伏安法檢測其氧化能力，來達到快速且能檢驗大量樣本之特性，期望能在未來做出可快速篩檢之檢測HDL功能的檢測工具。

In this study, the co-precipitation method was used to synthesize iron nanoparticles, and then the iron nanoparticles were reacted with TEOS and APTES to form a silica shell with amine groups on the outer bread, and then nanoparticles with a size of about 150 nm were obtained. Add EDC/NHS to react with Protein G to form an Amide bond, and then through the bioaffinity binding of Protein G and IgG, the antibody reacts with the antigen to achieve the function of quickly grabbing and purifying HDL.
In this study, we successfully synthesized 4.25 μg of antibody grafted with magnetic nanoparticles per mg, and achieved a stable capture of 159.1 μg/100ug HDL. Compared with the HDL capture rate of iron nanoparticles synthesized in the previous laboratory, APTES, L-Cystein.The iron nanoparticles modified by Cystein and Thioglycolic acid as protective groups only have capture rates of 3.5 μg/100uL, 12.5 μg/100uL HDL, and 49.46/ug/100ul respectively. The silicon dioxide magnetic core with modified amino groups used in this article Shell nanoparticles have the best HDL capture rate.
Next, the purified HDL samples from 9 patients obtained from Beiyi were tested by electrochemical cyclic voltammetry and compared with the HDL fluorescence oxidation value measured by Beiyi. Statistical analysis showed that the Pearson's correlation coefficient was 0.98. , Showing a strong correlation.Finally, we conducted a further study between the oxidation value and the cardiovascular disease-related factors endothelial progenitor stem cells concentration, and the trend was consistent with literature trend.
Finally, remove the plasma of the patient’s blood, add the purified HDL, mix it evenly, grab it with nanoparticles, and measure the oxidation capacity by electrochemical cyclic voltammetry, and compare it with the HDL fluorescence oxidation value measured by Beiyi , Statistical analysis shows that Pearson's correlation coefficient is 0.976, which also shows a strong correlation.
In this regard, this research seeks to replace the old time-consuming method of purifying HDL. By using Protein G and antibodies, the HDL in the blood can be quickly separated, and its oxidation ability can be detected by electrochemical cyclic voltammetry to achieve rapid and efficient performance. To test the characteristics of a large number of samples, it is hoped that in the future, a rapid screening test tool for HDL function can be made.

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