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研究生: 劉謹慈
Chin-Tzu Liu
論文名稱: 包載雙硫侖與超順磁性氧化鐵之雙功能載體粒子合成:臨床前卵巢癌動物模式治療評估
Dual-Functional Carrier Particles with Co-Encapsulation of Disulfiram and SPIOs:In Vitro and In Vivo Studies
指導教授: 白孟宜
Meng-Yi Bai
口試委員: 鄭詠馨
Yung-Hsin Cheng
葉伊純
Yi-Cheun Yeh
學位類別: 碩士
Master
系所名稱: 應用科技學院 - 醫學工程研究所
Graduate Institute of Biomedical Engineering
論文出版年: 2021
畢業學年度: 109
語文別: 中文
論文頁數: 150
中文關鍵詞: 卵巢癌靛氰綠紫草素磁熱治療雙硫侖超順磁性氧化鐵海藻酸鈉藥物載體
外文關鍵詞: Ovarian cancer, Indocyanine green, shikonin, magnetic hyperthermia, disulfiram, superparamagnetism, sodium alginate, drug carrier
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中文摘要 I ABSTRACT II 致謝 III 目錄 IV 圖目錄 VII 表目錄 X 中英文縮寫對照表 XI 1 背景與目的 1 2 實驗設計 3 3 文獻回顧 5 3.1 電噴霧於藥物傳輸上的應用 5 3.1.1 電噴霧(Electrospray) 5 3.1.2 載體系統(Delivery vehicles) 6 3.1.3 海藻酸鈉(alginate) 7 3.2 卵巢癌概要 9 3.2.1 卵巢癌統計 9 3.2.2 卵巢癌成因與治療 10 3.3 雙硫侖於癌症上的應用 11 3.4 熱治療於癌症上的應用 12 3.5 紫草素於癌症上的應用 13 3.6 靛氰綠於螢光標定上的應用 15 4 實驗藥品與儀器設備 17 4.1.1 藥品與材料 17 4.2 儀器與設備 19 5 實驗方法 21 5.1 實驗組別名詞定義 21 5.2 載體顆粒製備 22 5.2.1 藥品配置 22 5.2.2 電噴灑 23 5.3 材料分析 26 5.3.1 顆粒大小統計與形貌分析 26 5.3.2 掃描式電子顯微鏡(SEM)分析 27 5.4 藥物包覆率與藥物釋放 28 5.4.1 配置模擬體液(SBF) 28 5.4.2 雙硫侖與超順磁性氧化鐵系統藥物包封率與藥物釋放 29 5.4.3 紫草素與靛氰綠系統藥物包封率與藥物釋放 30 5.5 細胞實驗 33 5.5.1 細胞培養 33 5.5.2 細胞計數(Cell Counters) 37 5.5.3 細胞存活率分析(MTT assay) 38 5.6 螢光測試(紫草素與靛氰綠系統) 44 5.7 磁熱治療(雙硫侖與超順磁性氧化鐵系統) 45 5.7.1 磁熱治療原理與操作 45 5.7.2 超順磁性氧化鐵的升溫曲線 46 5.7.3 對細胞進行熱治療 46 5.8 動物實驗(雙硫侖與超順磁性氧化鐵系統) 48 5.8.1 實驗動物 48 5.8.2 動物腫瘤模式建立 48 5.8.3 CP70卵巢癌動物模型治療方法 50 5.8.4 SKOV3卵巢癌動物模型治療方法 52 5.8.5 安樂死 54 5.8.6 解剖與切片 54 5.9 統計方法 55 6 雙硫侖與超順磁性氧化鐵系統實驗結果 56 6.1 載體型貌分析 56 6.2 載體藥物包覆率與藥物釋放 57 6.3 細胞存活率分析(MTT assay) 57 6.3.1 超順磁性氧化鐵磁加熱毒性分析(Free form) 57 6.3.2 雙硫侖毒性分析(Free form) 58 6.3.3 載體毒性分析(Co-Particle) 58 6.4 動物實驗 59 6.4.1 CP70動物模型治療效果評估 59 6.4.2 CP70動物模型病理切片判讀 60 6.4.3 SKOV3動物模型治療效果評估 60 6.4.4 SKOV3動物模型熱治療效果評估 61 6.4.5 SKOV3動物模型病理切片判讀 61 7 紫草素與靛氰綠系統實驗結果 63 7.1 形貌分析 63 7.2 載體藥物包覆率與藥物釋放 63 7.3 細胞存活率分析(MTT assay) 64 7.3.1 紫草素毒性分析(Free form) 64 7.3.2 載體毒性分析(Co-Particle) 65 7.4 螢光測試(紫草素與靛氰綠系統) 65 8 結果與討論 66 8.1 雙硫侖與超順磁性氧化鐵系統 66 8.2 紫草素與靛氰綠系統 69 8.3 總結 70 9 附件與圖片 71 10 參考文獻 122

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