本研究由幾丁聚醣與N-環氧基-2,3-丙基-N-甲基-N,N-二烯丙基銨甲基磺酸化銨(N-epoxy-2-3-propyl-N-methyl-N-N-diallylammonium methylsulfate；MDAA)反應得到O-(2-羥基)丙基-3-甲基-二丙烯基銨幾丁聚醣磺酸化物(O-HDACS)，再分別與3種巰基化合物反應而得巰基化幾丁聚醣衍生物(THIOCS)，其目的是為了強化幾丁聚醣的抗菌性及生物相容性。所得之三種巰基化幾丁聚醣衍生物O-HDACS-MA、 O-HDACS-PA及O-HDACS-TA，以傅立葉轉換紅外光光譜儀(FT-IR)分析其分子結構；核磁共振分析儀(NMR)鑑定分子中化學官能基團的數目和種類；熱重分析儀(TGA)測試熱裂解溫度；介達電位(Zeta potential)分析幾丁聚醣及衍生物之電位性質；以DPPH測定其自由基抑制率；巰基化修飾率來判斷其接枝率；溶解度測定基本性質。並以金黃色葡萄球菌(Staphylococcus aureus)測試最小殺菌濃度(MBC)。另外用老鼠纖維母細胞(L929)測試其細胞成長活性與細胞毒性，期盼未來能應用於新型藥物釋放載體、傷口敷材及其他生醫領域。

In order to improve the antibacterial activity and biocompatibility of chitosan, chitosan was reacted with N-epoxy-2, 3-propyl-N-methyl-N, N-diallyl ammonium methyl sulfate (MDAA) to form O-(2-hydroxyl) propyl-3-methyl-diallyl ammonium chitosan sulfonates (O-HDACS). Afterward, thiolated chitosan derivatives (THIOCS) were prepared by reacting O-HDACS with three kinds of mercapto compounds and resulted in three kinds of THIOCS (O-HDACS-MA, O-HDACS-PA and O-HDACS-TA). These THIOCS were characterized using Fourier transform infrared spectroscopy (FTIR) to determine the chemical structures, Nuclear magnetic resonance (NMR) to analysis the molecules’ chemical function group of number and type, Thermogravimetry Analysis (TGA) to measure their pyrolysis temperature, Zeta potential to determine their surface charges, DPPH to measure radical inhibition rate, thiolated modification rate to determine the grafting rate. The solubility in water is also measured. The antibacterial activity was evaluated by the minimum bactericidal concentration (MBC) against Staphylococus aureus (S. aureus). The cell proliferation and cytotoxicity were evaluated with mouse fibroblasts (L-929). The results showed these three THIOCS can be applied as drug delivery carriers, wound dressing materials, and other biomedical fields in the future.

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