本研究為設計一具電場響應之生物相容性藥物載體模型，透過交流感應電場誘導帶電奈米粒子之電泳行為，進而加速藥物之釋放。聚多巴胺中空粒子是透過將多巴胺溶液自發氧化聚合到聚苯乙烯粒子模板上，接著透過反應時間的不同來得到不同厚度的聚多巴胺殼層，最後利用THF蝕刻聚苯乙烯內核得到聚多巴胺中空粒子。聚苯乙烯粒子則是透過無乳化劑聚合得到均一粒徑的粒子，再透過苯乙烯單體濃度的改變可以得到不同大小的聚苯乙烯粒子。由TEM影像圖中可以發現粒子呈現中空的形貌。利用DLS探討不同參數下的粒徑可以推算出中空粒子的厚度約為19、48與71奈米。最後分別利用親水性藥劑羅丹明B (Rhodamine B, RhB)與疏水性藥劑尼羅紅(Nile Red)對聚多巴胺中空粒子進行標記，由UV-Vis測量可得RhB和Nile Red在0.5mg/ml的藥物濃度下皆於厚度最小19nm的奈米粒子有最大的藥物裝載率，皆有大於95%藥物裝載率，並在一個月後僅有5%以內的藥物損失量。厚度19nm之聚多巴胺中空粒子在交流電場下可以得到最高的藥物釋放率，而相較於無使用電場能夠促進藥物釋放近30%。
將聚多巴胺中空粒子與RAW264.7小鼠巨噬細胞共培養，藉由MTT與CCK-8相互佐證下證明多巴胺中空粒子對於細胞是無毒性的。藉由CLSM觀察細胞攝取載體之螢光影像，再藉由施加交流電場觀察細胞中藥物釋放的效果。
本篇研究成功製備出具有電場響應之藥物載體模型，能透過交流電場誘導改變聚多巴胺中空粒子與藥物相互作用加速藥物釋放。電場響應藥物運送系統能夠於特定的部位和時間釋放，未來於藥物運送系統具有相當之潛力。

In this study, we prepared a biocompatible drug carrier model with electric-field response system. To achieve on demand drug release via electric field. Polydopamine hollow nanoparticles (PDA h-NPs) were synthesized by the spontaneous oxidative polymerization of dopamine solution onto polystyrene nanoparticles (PS NPs) template, followed by removal of the template. Different wall thickness can be controlled from different reaction time of dopamine polymerization. PS NPs were prepared by emulsifier-free emulsion polymerization, different particles size can be controlled from different styrene concentration. The wall thickness of PDA h-NPs can be simply controlled from 19 to 71 nm, depending on dopamine polymerization time. Moreover, high loading efficiency 96.1% and 97.8% of RhB and Nile Red for 19nm thickness PDA h-NPs. The PDA h-NPs maintained relatively high stability that only loss 9% drug amounts in one month. Furthermore, the release rate of hollow particle with thickness 19 nm can reach 32.3% under electric field in 60 minutes.
The PDA h-NPs were co-cultured with RAW264.7 cell. We used MTT assay and CCK-8 to confirm that PDA h-NPs were non-toxic polymer. We observed the cellular uptake and the effect of drug release under the electric-field from CLSM image.
In these results, applying polydopamine hollow particle in electric-field drug delivery represent an effective method for attaining spatiotemporal control of drug release at the desired site.

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