近年來氧化石墨烯在材料領域中掀起一股研究風潮，同時其在生醫領域上的應用也被廣泛研究。本論文採用簡易的物理方法製備奈米級的氧化石墨烯(GO100)；同時亦成功地合成並鑑定出由環糊精修飾的奈米級氧化石墨烯(GO100-CD)。此外探討GO100以及GO100-CD作為藥物載體，攜載抗癌藥物doxorubici(DOX)的效率。結果顯示GO100-CD對於DOX的攜載效率始終高於GO100，原因來自於GO100-CD中的環糊精可有效地包覆DOX；而GO100則是藉由π-π 堆疊作用力、輸水作用力、氫鍵以及靜電作用力以達到攜載DOX的效果。GO100-CD以及GO100在體外藥物釋放的結果中，顯示出與環境中的酸鹼值強烈相關，進一步指出在藥物載體攜載系統中其特定標靶的治療效果。
當GO100-CD以及GO100攜載DOX後，發生DOX的螢光淬滅行為。然而令人驚訝的是，藉由第四代聚乙二胺樹枝狀高分子(G4-Den)鍵結修飾在GO100表面後，螢光強度恢復了約一倍。此外結果顯示出較晚鍵結在GO100表面的G4-Den不會與已攜附的DOX競爭GO100的表面環境，進而得到在有/無G4-Den存在下，相同的藥物攜帶量。值得注意的是，據我們所知這是第一篇有關於藥物攜載系統中，恢復螢光淬滅後之螢光的報告。此外在改變G4-Den的添加順序後，依然發生DOX螢光淬滅行為，結果顯示出螢光淬滅行為與G4-Den的存在無關。這進一步證實為何提升螢光淬滅後的強度只發生在GO100/DOX/G4-Den或是 GO100-CD/DOX/G4-Den的系統。所有的研究結果顯示出，本篇論文發表了一個有趣的藥物載體系統。

In recent years, graphene oxide has become a prevailing topic in the materials community and its biomedical applications are investigated explosively. Here, the simple physical route for the preparation of nano sized graphene oxide (GO100) and the synthesis and characterization of cyclodextrin-immobilized graphene oxide (GO100-CD) were successfully performed. Furthermore, the controlled loading of doxorubicin (DOX), an anticancer drug, onto GO100 and GO100-CD was investigated. It was demonstrated that the loading capacity of DOX on GO100-CD was always higher than on GO100, and the difference comes from the inclusion of DOX in CD of GO100-CD, while the loading of DOX on GO100 can occur via π─π stacking, hydrophobic, hydrogen bonding, and/or electrostatic effects. Release profile showed strong pH dependence, indicating the specified therapeutic efficiency as a drug delivery.
Fluorescence of free DOX was quenched after it was loaded on GO100 and GO100-CD. However, surprisingly, the reenhancement of fluorescence by about one order was obtained through the conjugation of fourth generation amine-terminated poly(amido amine) dendrimer (G4-Den) on GO100 surface. Besides, it was found that the later loading of G4-Den will be not competitive with the previously adsorbed DOX on GO100 domain, resulting in the same DOX loading capacity between with and without G4-Den loading. Notably, to the best of our knowledge, this is the first report concerning to the reenhancement of the quenched DOX fluorescence in drug delivery system. Moreover, the altering in G4-Den adding order happened the quenching of DOX fluorescence independent of the existence of G4-Den. This supports that the fluorescence reenhancement only occurred in GO100/DOX/G4-Den or GO100-CD/DOX/G4-Den. All these observations suggest that present system offers an exciting mode of delivering drugs.

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