研究生: |
蕭諭農 Yu-Nong Hsiao |
---|---|
論文名稱: |
咪唑功能化的水溶性螢光共軛高分子具有可調控的氣體響應性應用於生物影像 Imidazole-Functionalized Water-soluble Fluorescent Conjugated Polymer as Controllable Gas-responsiveness for Biological Imaging. |
指導教授: |
鄭智嘉
Chih-Chia Cheng |
口試委員: |
謝永堂
Yeong-Tarng Shieh 陳建光 Jem-Kun Chen 邱智瑋 Chih-Wei Chiu |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 應用科技研究所 Graduate Institute of Applied Science and Technology |
論文出版年: | 2022 |
畢業學年度: | 110 |
語文別: | 中文 |
論文頁數: | 149 |
中文關鍵詞: | 咪唑 、共軛高分子 、氣體響應性 、二氧化碳 、生物影像 |
外文關鍵詞: | Imidazole, Conjugated polymer, Gas-responsiveness, Carbon dioxide, Biological imaging |
相關次數: | 點閱:321 下載:0 |
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本研究以氯化鐵氧化聚合方法成功合成出以疏水性聚噻吩為主體(作為發光骨架)及親水性的咪唑基團組成的水溶性共軛高分子。聚噻吩具有良好的光、電特性,可於巨觀與微觀條件下觀察螢光表現,而具有親水性的咪唑側鏈作為氣體響應性的官能基團,可以在水溶液中自組裝形成奈米微胞,並且經過二氧化碳與氮氣交替鼓泡入水溶液中,可以展現出具有回復性質的顆粒粒徑、表面電位與螢光特性,並且此一回復性質可以持續多次仍維持高穩定性。在體外細胞實驗中,pH 7.4、37°C生理條件下,在正常細胞與癌細胞中均展現出低細胞毒性;在細胞的螢光影像與流式細胞儀的研究表明,在二氧化碳鼓泡後,能展現出更高的細胞螢光強度與細胞攝取率,證實本實驗的實驗材料在未來有極佳潛力應用於生醫螢光探針等領域。
In this study, a water-soluble conjugated polymer composed of a hydrophobic polythiophene as the main body (as a light-emitting skeleton) and a hydrophilic imidazole group was successfully synthesized by the oxidative polymerization method of ferric chloride. Polythiophene has good optical and electrical properties, and its fluorescence performance can be observed under macro and micro conditions. The hydrophilic imidazole side chain, as a gas-responsive functional group, can self-assemble in aqueous solution to form nano-microstructures. After carbon dioxide and nitrogen are alternately bubbled into the aqueous solution, it can exhibit particle size, zeta potential and fluorescence characteristics with recovery properties, and this recovery property can continue for many times and still maintain high stability.
In the in vitro cell experiment, under physiological conditions of pH 7.4 and 37°C, it showed low cytotoxicity in normal cells and cancer cells. Fluorescence images and flow cytometry showed that after carbon dioxide bubbling can show higher cell fluorescence intensity and cell uptake rate, it confirmed that the experimental materials in this research have great potential to be used in the fields of biomedical fluorescent probes in the future.
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