本實驗室先前研究使用光固化型PCL (PCL-DA)結合光交聯劑PEG-acrylate以及光起始劑，利用直照式動態光罩快速成型系統製作3D組織工程支架，由於材料對光敏感性較高，導至支架疊層至三層時會造成孔徑封閉，故本研究建構反射式動態光罩快速成型系統，來改善這些缺點。此外，利用PEG-diacrylate (PEG-DA)取代PEG-acrylate，可使交聯時的鍵結更穩固藉此特性來提升支架強度。
為了尋找最佳的PCL-DA/PEG-DA混合比例，將材料分為6:4、7:3與8:2三個比例進行性質測試，量測抗拉強度與接觸角。量測結果顯示新材料在抗拉強度的表現上優PEG-acrylate，且材料在親水性的表現上優於未添加PEG-DA之PCL-DA。本研究新建構的反射式動態光罩快速成型系統是將DLP投影機投射的光路反射至材料槽，來降低光照度藉此來改善支架成型精度。利用三種不同比例之材料製作單層支架，其中以比例為8:2之材料，其誤差更可控制在2%以內；利用比例為8:2之材料製作出重現性高的100μm孔徑之單層支架，其誤差可控制在3%以內，並以此利用偏移堆疊的方式製作3D組織工程支架，有效的將堆疊層數由先前的兩層提升至五層。本研究有效的利用PCL-DA/PEG-DA搭配反射式動態光罩快速成型系統使用偏移堆疊方式製作3D組織工程支架

In our previous research, photo-curable PCL (PCL-DA), mixing with PEG-acrylate as a binder and photo-initiator, was photo-polymerized to fabricate tissue engineering scaffolds by a direct Dynamic Masking Rapid Prototyping (DMRP) system. The strong photo-sensitivity of the material system led pores closed in the scaffolds after stacking three layers. Therefore, in this research, a reflective DMRP system was established to improve the above issue. Moreover, PEG-diacrylate (PEG-DA) was used to replace PEG-acrylate to increase the strength of the cured materials.
In order to find suitable composition of PCL-DA to PEG-DA for fabrication, three ratios (6:4, 7:3, 8:2) were investigated. Tensile strength and contact angle were measured. The results showed great strength improvement compared to those use PEG-acrylate as a binder, and the materials are more hydrophilic compared to PCL-DA without PEG-DA. The new reflective DMRP system placed the DLP projector at the side and reflected the light to the material tank by a mirror to reduce illuminance and improve process accuracy. Materials with above ratios were used to fabricate single-layer scaffolds by the new system, and found the ratio of 8:2 has the least error (2%). This best material recipe was further used to generate 3D scaffolds through offsetting grid pattern in the alternating layer, and finally improve the scaffold's stacking layers from two to five. This research has successfully combined PCL-DA and PEG-DA to fabricate 3D tissue engineering scaffolds by reflective DMRP system, and can be applied to more scaffold applications in the future.

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