臨床醫學界常見的尿道外傷後或感染後引起的的狹窄一直是難以處理的臨床問題。新技術以自體黏膜組織重建切開後的尿道仍存在許多問題，如增加口腔手術、形成二次傷害、新陳代謝、表皮瘻管等，因此可考慮使用生醫材料進行替代。
　　本實驗選用之聚己內酯( Polycaprolactone, PCL)具有良好生物相容性以及柔軟度，能夠依需求進行修飾改良；蘆薈常見被使用於有機食品、醫學美容、以及敷料加工等等。天然蘆薈萃取物之調節免疫機制長期以來備受研究。另外，蘆薈萃取物能夠促進細胞貼附生長，抗發炎，增進傷口癒合，若是結合組織工程支架，期望可以緩解結痂發炎情形， 避免過度疤痕產生，也能夠減低病患痛苦。因此，本研究期望能夠利用表面修飾之多孔性PCL材料交聯蘆薈萃取物做為尿道組織工程支架，減緩術後發炎結疤情形，並促進組織新生。
　　本實驗分別針對材料，細胞結合材料以及動物模式探討。在利用交聯劑將蘆薈交聯於多孔性PCL支架表面後，除了觀察材料表面結構以及物理化學性測試，另針對蘆薈抗菌能力以及對細胞的生物相容性作討論。在動物模式中，首先觀察蘆薈汁液消炎能力，手術部分則切開大鼠尿道並縫補材料於缺口，經4、8週後犧牲動物進行組織切片，並利用IHC、Masson等特殊染色法定量發炎程度。
　　結果發現，多孔性PCL支架在交聯蘆薈萃取物後不會明顯改變整體物化性質，1%之蘆薈萃取物可有效抑制大腸桿菌生長；在細胞實驗上，1%蘆薈萃取物能促進細胞爬行生長；動物模式方面，雖然手術方式限制的關係使得傷口呈現開放式瘻管，但HE組織切片發現兩組之間皆無明顯發炎細胞聚集，特殊染色結果也證明蘆薈多醣能夠控制纖維母細胞增生，減少疤痕形成。
　　由以上實驗可知表面修飾後之多孔性PCL交聯蘆薈的確可促進細胞生長及組織成型，降低IL-6分泌及減少疤痕產生，顯示此新型組合之生醫材料可考慮做為修補中型傷口組織工程材料。

The Urethral Strictures generally caused by injury-related traumas or viral or bacterial infections have been problems in the clinical medicine. Ther e are many problems of new techniques using autotransplantation with mucosa for reconstruction, such as increasing oral surgeries, secondary damage, metabolism, or fistula, so they may be substitute with biomaterials.
Polycaprolactone(PCL) is known biocompatible, softness, and can be modified if requirements in this study; aloe is often applied to organic foods, medical beauties, and wound dressings. The immunomodulation mechanism of nature aloe extract has been studied for a long time. On the other side, aloe extract can improve cell adhesion, anti-inflammation, and increase wound healing; if combined with bio-scaffolds, it may relieve scars growing and reduce pain that patients suffer from. Therefore, this study expected that using the porous surface modified PCL scaffolds cross linked with aloe extract in urethra tissue engineering to reduce inflammation, scar producing followed by surgeries, and to improve tissue regeneration.
This experiment discussed with materials, cells seeded on materials, and animal models. Instead of surface structures, we conferred porous PCL scaffolds cross linked with aloe extract with physical and chemical properties, anti-bacterial, and cell biocompatible. In animal models, initially we observe anti-inflammatory of aloe; in surgery parts, we had incision on urethras of SD rats and sutured wound with our materials, followed by 4 and 8 weeks animals sacrificed, tissue sections, and using IHC, Masson special staining to quantify degree of tissue inflammation.
Results showed that the physical and chemical properties of porous PCL scaffolds would not be changed after aloe cross linking; 1 % aloe extract could inhibit E. co li growing efficiently; for cell part, 1 % aloe extract could improve cell migration and proliferation. Though inhibition of surgery method resulted in open wide fistula, however, it was not obviously found inflammatory cells gathering around the tissue between the HE sections of the two material groups, and the special staining also proof that the aloe extract could control fibroblasts proliferation and reduce scars producing.
The conclusions were that the aloe cross-linking on surface modified porous PCL indeed improve cell proliferation and tissue reconstruction, reduce IL-6 releasing and scars producing, showed that this new combination of biomaterial can be considered to be a medium size wound healing materials in tissue engineering.

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