本研究以褐藻膠粒子(alginate beads)來做為鈣離子釋放之載體並延遲鈣離子在褐藻膠中凝膠化的時間，並且在褐藻膠中加入膠原蛋白以促進細胞增長並混入PluronicR F127(F127)使此支架具備溫度敏感性，使得在37℃下能提升整體材料的黏滯性以及剛硬性。將褐藻膠鈣粒子(Beads)、高濃度褐藻膠、膠原蛋白以及F127以混摻的方式完成支架的製備。支架性質以動態機械分析儀(DMA)以及流變儀(Rheometer)測定其溫度敏感性質以及凝膠化前後的機械性質。鈣離子的均勻性可由紫外/可見光光譜儀(UV-Vis)測定Beads在茜素紅溶液中隨時間的釋放情形，並由光學顯微鏡觀察鈣離子的分佈情形。生物相容性則以纖維母細胞以及骨母細胞進行培養，以MTT assay進行細胞毒性測試並以Trypan Blue染色法進行細胞貼附實驗。本研究發現當樣品加入Beads後，鈣離子會與褐藻膠進行交聯反應而使得材料具有彈性，且在37℃下因F127展現其溫敏性而使得支架的黏滯性提升，另外，由於鈣離子隨時間的增長其釋放量增加，支架的剛硬性亦隨時間而增加。細胞毒性測試結果發現，含有膠原蛋白的材料對於纖維母細胞和骨母細胞的生長幫助並不大，含F127的樣品則有減緩生長的情形，但整體支架對於細胞而言是較無毒性的；細胞貼附測試的結果則發現，這次研究所採用的支架是有利於纖維母細胞和骨母細胞的貼附以及生長。因此初步評估此支架是可運用於骨材方面的應用。

In this study, alginate beads are used to be the carrier of calcium ions and delay the gelation of alginate. In order to facilitate the growth of cells, collagen was added into the alginate solution. PluronicR F127(F127) was also mixed with alginate solution to make a thermosensitive scaffold. The mechanical properties were measured by DMA and Rheometer, which displayed the thermosensitivity of gel and the modulus of gelation before and after. The evenness of calcium ions in gel was proved by UV-Vis measurements for calcium ions release in alizarin red S (ARS) solution. The distribution of calcium ions in alginate gel after dying by ARS solution was also observed by optical microscope. For biocompatibility, L929 fibroblasts and hFOB 1.19 osteoblasts were tested in this study. The cell toxicity test was done by MTT assay and cell adhesion test was proved by Trypan Blue staining. The results showed that the gelation occurred in a gradual manner by the change of modulus since the release of calcium ions increasing by time and cause ionic crosslinking which update the elasticity of gel. Thermosensitivity in gel also appeared under 37℃ because of adding F127. In addition, the growth of L929 fibroblasts and hFOB 1.19 osteoblasts evaluated non-toxicity in gel. The results from cell adhesion test also proved L929 fibroblasts and hFOB 1.19 osteoblasts can attach the surface of scaffold and grow. These results showed that this scaffold have possibility to applied in bone materials.

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