好氧顆粒汙泥在廢水生物處理的領域之中，被視為一具有前瞻性的技術。活性汙泥在SBR中培養80天，以進料負荷交替變化做為主要變化參數。本論文主要探討在進料負荷交替變化和細菌藻酸鹽含量之間的相關性，以及第二信使-環鳥苷二磷酸 (c-di-GMP) 在好氧顆粒化中的作用。
實驗結果顯示，好氧顆粒可在進料COD 為1250至5000 mg/l [有機負荷率4.344至17.379 Kg COD/(m^3．d) ] 之間交替變化運行的情況下快速地培養成功，同時其它因素控制不變。當進料負荷達到最高濃度時，可以提高細菌中c-di-GMP的表達，促進ALE合成，引發汙泥聚集反應以促進顆粒化。此外，顆粒在長時間操作後，顆粒菌相會趨於一致，包含有假單胞菌屬、梭狀芽孢杆菌屬、索氏菌屬和節桿菌屬。

Aerobic granulation, a novel environmental biotechnological process, has been drawing increasing interest in the area of biological wastewater treatment. The activated sludge is cultivated in the sequencing batch reactor (SBR) using the alternating feeding as a main variable parameter over 80 days operation. This study aims to investigate the correlation between the alternating feeding and the content of alginate-like exopolysaccharides (ALE), and to understand the role of c-di-GMP (bis-(3′-5′)-cyclic dimeric guanosine monophosphate) as a second messenger in aerobic granulation.
The experimental results reveal that when other conditions remain the same, the aerobic granules are rapidly and successfully cultivated by a method of alternating COD feed between 1250- 5000 mg/l [OLR between 4.344- 17.379 Kg COD/(m^3．d) ]. When the alternating feeding reaches the highest level, c-di-GMP expression by bacteria is raised. This, in turn, promotes the ALE synthesis, and the response of sludge aggregation is initiated to facilitate the granulation. Furthermore, the experimental results also reveal that the diversity of microbial community in the aerobic granules is predominantly uniform over a long-term operation, especially with strains of the genus Pseudomonas, Clostridium, Thauera and Arthrobacter.

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