將絲素蛋白SF (Silk Fibroin)用甲基丙烯縮水甘油酯GMA(Glycidyl methacrylate)化學修飾及加入具有彈性、交聯韌性的聚（丙二醇-二丙烯酸酯）(Poly(propylene glycol)diacrylate)PPG製作成，且用3D列印方式印出均勻交錯陣列，應用於人類非小細胞肺癌細胞系A549細胞進行模擬肺癌病患呼吸頻率造成其影響。在DLP 3D樹酯槽內直接放入SFGP生物墨水溶液進行打印，通過DLP光引發自由基逐層列印在層基板上，這樣的列印方式解決了傳統方法無法控制其結構和內部幾何形狀的問題，且對所需的內部架構、結構進行精確的控制，應用於生物材料領域，以滿足器官和組織的需求，且它提供了良好的細胞微環境和機械支撐能力。
在這裡，我們針對絲素蛋白和甲基丙烯酸縮水甘油酯 (SFGMA) 進行了改性，使用3D列印機須符合機台列印的黏度範圍，生物墨水需符合此區間，因此加入PPG用來增加黏彈性，並做了機性性質評估以及流變性測試，確認列印參數、了解物理特性，接著利用計算機輔助程式(AutoCAD)將模型逐層畫出製造3D規則排列模型，使用DLP 3D列印機列印出模型並進一步進行模擬肺癌病患呼吸頻率對肺癌增生之影響。
將A549肺癌細胞種在3D SFGP均一孔洞模型中，用週期性動態培養細胞拉伸儀進行分析驗證，這些結果表明，SFGP 3D孔洞結構無論在細胞相容性或細胞增殖都明顯高於無孔洞結構及控制組，另外測試結果也表明應變越大如同呼吸越大，對於A549細胞活性有顯著增加，在改變頻率部分，頻率越大，造成細胞增殖越快，由此實驗也可以提供臨床醫師在用藥上的建議。

In this study, the silk fibroin SF (Silk Fibroin) was chemically modified with GMA (Glycidyl methacrylate) and added with PPG(poly(propylene glycol) diacrylate) with elasticity and cross-linking toughness. 3D printing was used to print uniform staggered arrays, which were applied to human non-small cell lung cancer cell line “A549 cells” to simulate the effects of lung cancer patients' respiratory rate. The SFGP bio-ink solution is directly placed in the DLP 3D resin tank for printing,and the DLP light-induced free radicals are printed on the layer-by-layer substrate This printing method solves the problem that the traditional method cannot control It’s structure and internal geometry, and accurately control the required internal structure , it is used in the field of biomaterials to meet the needs of organs and tissues, and it provides a good cellular microenvironment and mechanical support capabilities.
We modified silk fibroin and glycidyl methacrylate (SFGMA). The 3D printer must meet the viscosity range of machine printing, and the bioink must meet this range. Therefore, PPG is added to Viscoelasticity was added, and organic property evaluation and rheological test were performed to confirm printing parameters and understand physical properties. Then, use computer-aided program (AutoCAD) to draw the model layer by layer to create a 3D regular arrangement model, and use DLP 3D printing. The model was then printed out and further simulated the effect of lung cancer patients' respiratory rate on the proliferation of lung cancer.
The A549 lung cancer cells were planted in the 3D SFGP homogeneous hole model and analyzed and verified by a periodic dynamic culture cell extensometer. These results show that the SFGP 3D hole structure is significantly higher than the non-hole structure in terms of cell compatibility and cell proliferation. and the control group. In addition, the test results also showed that the greater the strain, the greater the respiration, the significant increase in the activity of A549 cells. In the part of the frequency of change, the greater the frequency, the faster the cell proliferation. This experiment can also provide clinicians with medication. suggestion above.

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