本研究中主要將研究之方向分為二大主軸: (I) 合成三元金屬觸媒(Aurod-Pdshell-Ptcluster)探討於電化學式雙氧水氧化反應之感測分析。(II)製備葡萄糖與尿酸生物微型感測試片。
(I) 此研究成功以晶核成長還原法將兩種奈米金屬顆粒(Pt、Pd)沉積於金奈米桿上(Aurod-Pdshell-Pdcluster)，並具有良好的型態，同時本研究也利用XRD、UV、TEM、ICP以及XAS進行材料分析。雙氧水電化學感測器之製備方式以奈米金屬觸媒修飾於玻璃碳電極表面，並發現以Aurod-Pdshell-Pdcluster ¬所製備之雙氧水感測器的效能比商業化鉑觸媒(JM-Pt)來的高，並利用XAS、XPS以及活性表面積來證明。另外，從電化學測試上發現以Aurod-Pdshell-Pdcluster所製備出的雙氧水感測器具有良好的再現性、安定性與抗干擾能力。
(II) 建立最適化電極感測層之結構，並轉移至微型電極感測試片，利用添加微量交聯劑-戊二醛，使其形成網狀結構固定酵素於微型電極表面上，由葡萄糖與尿酸酵素動力學反應Michaelics-Menten constant分別為10.2 mM、0.7 mM與穩定性測試探討(電極保存30天後效能約減少1.5 %與11.4 %)等結果得知，製備之酵素感測器不僅能提供快速固定酵素於電極表面，所形成之三維空間網狀薄膜幫助酵素與待測物質間進行生物反應，使酵素具良好之親合性與穩定性。
本研究中製備之葡萄糖感測器於0.4 V v.s Ag/AgCl之施加電位下，具有感測葡萄糖雙線性範圍為0.5至7.4 mM (R2 = 0.99) 及7.4至22 mM(R2 = 0.99)，且其偵測靈敏度為49及23 μA/mMcm2，此觸媒感測器亦能使用於尿酸感測上，其製備之尿酸感測器於0.4 V v.s Ag/AgCl之施加電位下，具有感測尿酸線性範圍為0.0459 mM至1.91 mM (R2 = 0.99)，且其偵測靈敏度為55 μA/mMcm2，研究結果顯示，藉由合成之三元奈米金屬觸媒應用於生物感測器之製程系統，成功製備出具優良再現性與量測準確度、結構穩定之高靈敏度微型葡萄糖與尿酸感測試片。

This study is comprised of two parts: (I) The synthesis of trimetallic Aurod-Pdshell-Ptcluster nanocatalysts for the applications of electrochemical sensing of hydrogen peroxide. (II) Fabrication of glucose and uric acid thin film model sensor strip.
(I) A new highly catalytic and intensely sensitive amperometric sensor based on Pt and Pd nanoparticles (NPs) for the rapid and accurate estimation of hydrogen peroxide (H2O2) by electrooxidation in physiological conditions is reported. Pd and Pt NPs-decorated Aurod nanocatalysts (Aurod-Pdshell-Ptcluster) were prepared by a Seed-growth method, and were characterized by XRD, UV, TEM, ICP and XAS. The sensors were constructed by immobilizing Aurod-Pdshell-Ptcluster nanocatalysts on a glassy carbon electrode. The Aurod-Pdshell-Ptcluster sensor showed a better performance in H2O2 sensing than did the commercial (JM-Pt). Explanations were sought from XAS, XPS, ECSA measurements to explain the reasons for differences in sensor activity. In addition, the Aurod-Pdshell-Ptcluster nanocatalyst sensor electrode also exhibited excellent reproducibility and stability. Along with these attractive features, the sensor electrode also displayed very high specificity to H2O2 with complete elimination of interference from UA, AA, AAP and glucose. The biosensor was constructed by immobilizing the Aurod-Pdshell-Ptcluster catalysts in Polypyrrol film on the Au/Ti micro-strip working electrode surface. An inner Polypyrrol film coating was used to eliminate common interferences such as UA, AA, AAP and glucose. Finally, an enzyme layer was fabricated by electrostatic adsorbed method. In addition, the biosensor exhibited high reproducibility, good storge stability and satisfactory anti-interference ability.
(II) The sensing layers with optimum structure were developed and fabricated on mini-sensor strips. The additions of cross-linking agent to ensure the combination . The solution recipe and parameters for electrochemical polymerization of pyrrole were optimized. The enzyme kinetics of glucose and uric acid (Michaelics-Menten constant = 10.3 and 0.7 mM, respectively) and stability tests have been demonstrated that the sensitivity decay of the assembled electrodes is around 1.5 % and 11.4 % after 30 days. It shows that the assembled sensor prepared under this condition provides mild environment for enzyme immobilization and 3D network membrane facilitate the bio-reaction between enzyme and bio-species, which makes enzyme exhibiting good affinity and stability.
The prepared glucose sensor exhibits sensitivity of 49 μAmM-1cm-2 in detection ranges between 0.5 to 7.4 mM, and sensitivity of 23 μAmM-1cm-2 in detection ranges between 7.4 to 22 mM (R2 =0.990) at 0.4 V (vs. Ag/AgCl). On the other hand, the uric acid sensor also exhibits a sensitivity of 55 μAmM-1cm-2 in detection ranges between 0.0459 to 1.91 mM (R2 =0.990) at 0.4 V (vs. Ag/AgCl). The results show that the glucose and uric acid strips with a high sensitivity, reproducibility and stability was successfully prepared.

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