本研究目的為設計一種複合式、高檢測效率的奈米級纖維，經由表面改質使其具有專一性，用來檢測大腸癌病患血液中的循環腫瘤細胞(CTCs) 。然而在循環腫瘤細胞檢測時常會受大量的白血球影響檢測效率，故本研究使用聚氨酯(Polyurethane；PU)、聚乙烯乙二醇(Poly(ethylene oxide)；PEO) 60/40之最佳混合比例所構成奈米纖維薄膜，以PU做為基底材料用來減緩檢測時奈米纖維水解狀況，而PEO作為生物抗沾黏材料，經過表面改質後仍有生物抗沾黏功能可避免白血球沾附。PEO及PU由(3-aminopropyl)triethoxysilane (APTES)修飾後帶有NH2官能基可固定Streptavidin和anti EpCAM -biotin來偵測CTCs，將此兩種材料以不同的比例進行混合並利用靜電紡絲技術製造出奈米纖維薄膜來達到不易水解、生物抗沾黏且富含高劑量的anti EpCAM抗體的目的。
在測試奈米纖維抗白血球沾黏的能力中發現，PU/PEO(60/40)平均具有90%的抗白血球沾黏能力。而在三種大腸癌細胞株(HCT-116、DLD-1、HT-29)測試抓取能力中結果發現本材料可在低濃度的CTCs中達到七成的抓取率。
進行抓取試驗後，將試片與流式細胞儀以相同實驗條件進行對比試驗，發現流式細胞儀偵測10顆癌細胞的誤判率超過五成，遠大於本材料試片，而最後在臨床兩位病人檢體實驗中發現，成功捕捉到其每毫升血液中CTCs數目分別為6、3顆。在捕捉循環腫瘤細胞CTCs上所面臨的阻礙，包含步驟繁雜、高成本、捕捉效率等，故本實驗以設計低成本、快速檢測優勢的奈米纖維薄膜為目的，對於臨床上稀少的循環腫瘤細胞偵測是具備高度發展潛力的。

In this study, we fabricated a composite and high detective efficiency nanofiber via surface modification to have specificity for detecting circulating tumor cells(CTCs) in peripheral blood of colon cancer patients. However, CTC detection is always influenced by extensive white blood cells (WBC) in human blood. The composite electrospun nanofiber was made from polyurethane(PU) and Poly(ethylene oxide)(PEO) and its best ratio was 60/40. PU was a substrate which could avoid the nanofiber dissolving in aqueous solution. PEO was a kind of anti-fouling polymer that can prevent from WBC adhesion. The composite nanofiber was modified by (3-aminopropyl)triethoxysilane (APTES) to graft amine group. Amine groups were the media to immobilize antibody biotin labeling (anti-EpCAM biotin) for CTC capture. Mixing PU and PEO to fabricate nanofiber via electrospinning according to the antibiofouling and analyte properties.
First, the nanofiber possessed 90% antifouling property for WBC. Colon cancer cell line (DLD-1, HCT-116 and HT-29), low concentration colon cancer cells were exploited mixed in medium to perform the capture of CTCs. The results exhibit that detection the capture of CTCs. The results exhibited that detection efficiencies increased linearly upon ca. 70%.
Then the composite nanofiber and flow cytometry in the same experimental conditions were compared for CTC capturing test. The results showed that the false rate of detecting 10 cancer cells(HCT-116) was over 50% and much higher than our composite nanofiber,
Finally, peripheral blood of two colon cancer patients was exploited to examine the property of CTC capture using the naofiber in clinical test.The nanofibers could capture 6 and 3 CTCs per ml from the two patients repectively in the clinical test. The current obstacles for CTC capture in peripheral blood included multiple procedural steps with high cost. Therefore, the nanofiber could possessed high potential to capture CTCs clinically with lower cost and fast detection.

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