藍藻蛋白與海藻酸鹽皆具有良好的生物相容性，本研究期望結合藍藻蛋白與海藻酸鹽本身高含水量的特性，以促進纖維母細胞之生長，製備適合用於傷口癒合之敷材。同時嘗試加入碳酸鈣微粒，期望提升機械強度，最終獲得可促進傷口癒合之複合敷料。
　　本研究將非水溶性藍藻蛋白與海藻酸鹽摻合形成共聚溶液後，以氯化鈣溶液固化，製備含不同比例非水溶性藍藻蛋白之複合水凝膠。之後，使用FTIR觀察成膠後官能基變化，利用坂口實驗(Sakaguchi test)測量非水溶性藍藻蛋白之含量，並以掃描式電子顯微鏡(scanning electron microscope，SEM)觀察複合水膠之表面與橫切面結構，以萬能材料試驗機(universal testing machine)進行機械性質測試，並進行含水率測試(swelling test)。細胞實驗則以纖維母細胞L929進行培養，以MTT 分析比較不同膠體之細胞生長情形，並以細胞遷移(cell migration)實驗模擬傷口癒合。
　　結果顯示，適量摻合並加入碳酸鈣微粒有助提升機械性質，並且非水溶性藍藻蛋白可有效促進細胞生長與遷移，可視為具有傷口敷料潛力之生醫材料。

Both multi-L-arginyl-poly-L-aspartate (MAPA), also known as cyanophycin, and alginate have shown good biocompatibility. This study aims to combine MAPA and alginate to prepare a composite hydrogel that could promote wound healing. Calcium carbonate micro particles were also incorporated in an attempt to increase the mechanical properties of the hydrogel.
In this study, insoluble MAPA (iMAPA) and alginate were blended to form a hydrogel with calcium chloride solution at different ratios of iMAPA/alginate. The gel properties were examined by FTIR, and the content of iMAPA was measured by Sakaguchi method. Scanning electron microscope (SEM) was used to observe the morphology of surface and cross-section of the composite hydrogel, and the universal testing machine was employed to measure the mechanical properties of stretching and compression. The swelling ratio of composite hydrogels was also examined. Fibroblast was employed in the cell experiment and the cell growth on different composite hydrogels was compared by MTT assay. The cell migration experiment was used to mimic a wound in an effort to examine the effect of composite hydrogels on wound healing.
The above results showed the addition of calcium carbonate micro particles can effectively improve the mechanical properties, and iMAPA can effectively promote cell growth and migration. The composite hydrogel showed a potential for the application of wound dressing.

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