本研究選用溫度敏感性材料N-isopropylacrylamide (NIPAM)，並與Acrylamide (AM)作自由基加成聚合，其中調整AM的含量，製備成不同比例的poly(N-isopropylacrylamide-co-acrylamide)(P(NIPAM-co-AM))，並將其作為高分子主鍊，之後再添加3-glycidyloxypropyltrimethoxysilane (GLYMO)及Tetraethyl orthosilicate (TEOS)，利用GLYMO與AM作開環反應，再利用GLYMO與TEOS經溶膠-凝膠法(Sol-Gel reaction)，而得到最終產物之溫度敏感型複合奈米粒子P(NIPAM-co-AM)_Silica hybrid nanoparticles，並評估此奈米粒子應用於藥物傳遞系統之可行性。
因本研究所合成的複合奈米粒子具有溫度敏感性，當溫度低於低臨界相分離溫度(lower critical solution temperature, LCST)時，使高分子鍊伸展開來，奈米粒子會呈現親水性，且於環境pH 7.4下，材料帶負電荷，能夠與帶正電的DOX經靜電荷交互作用而相互吸附，反之，若溫度高於LCST時，會呈現疏水性，我們利用此特性，於低溫下進行。不同的AM比例，藥物包覆效率約為45 ~ 55%。
藥物釋放的部分，於不同的酸鹼度下進行，如pH 7.4、pH 6及pH 5，且將環境模擬人體生理溫度37"℃" ，此時複合奈米粒子會收縮，將藥物包覆在核心中，但由於藥物會受到內部擠壓，且酸性的環境會提供質子，此時藥物會藉由質傳釋放出來，並於120小時內的釋放率，不同的AM比例，於pH 7.4、pH 6及pH 5分別達到40%、70%及80%左右。
最後，將複合奈米粒子包覆DOX之樣品與癌細胞(HeLa cells)共同培養，並對此癌細胞進行細胞毒性分析及細胞吞噬分析，綜合以上，探討此複合材料在癌症治療之藥物傳遞系統的應用價值。

In this research, the thermal-sensitive material NIPAM was used, and Acrylamide (AM) was used as a free radical addition polymerization. The content of AM was adjusted to prepare different ratios of poly(N-isopropylacrylamide-co-acrylamide) (P(NIPAM-co-AM)), and as a backbone. Adding 3-glycidyloxypropyltrimethoxysilane (GLYMO) and Tetraethyl orthosilicate (TEOS) Tetraethyl orthosilicate (TEOS), use GLYMO and AM for ring-opening reaction, and use GLYMO and TEOS by Sol-Gel Reaction to get the final product The thermal-sensitive hybrid nanoparticles P(NIPAM-co-AM)_Silica. It was used and the feasibility of applying the nanoparticles to the drug delivery system was evaluated.
Because the hybrid nanoparticle synthesized in the research is temperature sensitive. When the temperature is lower than the low critical solution temperature (LCST), the polymer chain is swelling, and the nanoparticles are hydrophilic. At ambient pH 7.4, the material will have a negative charge and can interact with the positively charged DOX via electrostatic interaction. Conversely, if the temperature is higher than LCST, it will be hydrophobic. We use the property to do load drug. It is carried out at low temperature, the drug coating efficiency is about 45 - 55% for different AM ratios.
The drug release is carried out under different pH value, such as pH 7.4, pH 6 and pH 5, and the environment is simulated at 37 °C. The hybrid nanoparticles will shrink and the drug is coated in the core. However, because the drug will be squeezed internally, and the acidic environment will provide protons, the drug will be released by mass transfer, and the release rate in 120 hours, different AM ratios, at pH 7.4, pH 6 and pH 5 reached 40%, 70% and 80% respectively.
Finally, the drug-coated composite nanoparticles were co-cultured with cancer cells (Hela cells), and the cancer cells were analyzed for cytotoxicity and phagocytosis. In summary, exploring the application value of this hybrid material in drug delivery system for cancer treatment.

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