氧化鋅奈米粒子(ZnO NPs)藉著使用水熱法，然後再經過不同溫度下的退火 (200,
300, 400, 500, 600 and 700oC)被成功的合成出來。 並且使用金奈米粒子(Au NPs)透過二巰基
丁二酸(DMSA) 固定於氧化鋅奈米粒子合成出金-氧化鋅奈米粒子(Au-ZnO NPs)後，應用於
光降解(photodegradation)和表面增強拉曼散射(SERS)檢測水汙染物(如：亞甲藍)。另外，
也探討氧化鋅奈米粒子在不同退火溫度下的粒徑效應。透過掃描電子顯微鏡（SEM）以及
穿透電子顯微鏡（TEM）驗證氧化鋅奈米粒子和金奈米粒子的形態。結果顯示兩種粒子
皆為球型，氧化鋅奈米粒子直徑約為50 nm，金奈米粒子直徑約為10 nm。通過紫外 - 可見
光譜驗證氧化鋅奈米粒子的光學性質。XRD 結果顯示氧化鋅奈米粒子具有高度的結晶性，
為六方纖鋅礦晶體結構。金-氧化鋅奈米粒子在光降解和 SERS 檢測中表現出很好的特性，
這可以應用於生物醫學、水處理以及快速檢測領域。

Zinc oxide nanoparticles (ZnO NPs) were successfully synthesized by hydro thermal
methods and then annealing at varied temperature (200, 300, 400, 500, 600 and 700oC).
Furthermore, gold nanoparticles (Au NPs) were immobilized on the dimercaptosuccinic acid
(DMSA) modified ZnO NPs (Au-ZnO NPs) for the photodegradation and surface enhanced Raman
scattering (SERS) detection of the water pollutants, such as methylene blue. The effects of the
particle size of ZnO NPs at varied annealing temperatures were investigated. The morphology of
ZnO NPs and Au NPs were evaluated by scanning electron microscopy (SEM) and transmission
electron microscopy (TEM). It showed that ZnO NPs were spherical in shape with the diameter
about 50 nm and Au NPs were spherical in shape with diameter about 10 nm. The optical properties
of the ZnO NPs were characterized by UV-visible spectroscopy. XRD results revealed that the
ZnO NPs are highly crystalline, showing the hexagonal wurtzite crystal structure. Au-ZnO NPs
displays great characterizations in the photodegradation and SERS detection, which is potential to
apply in the field of biomedical and water treatments and rapid detection.

[1] Gust, D.; Moore, T. a; Moore, A. L. Acc. Chem. Res. 2009, 42 (12), 1890–1898.
[2] Srivastava V, Gusain D, Sharma YC. Synthesis, characterization and application of
zinc oxide nanoparticles (n-ZnO). Ceramic International, vol. 39, 2013, pp. 9803-
9808.
[3] Cao GZ. Growth of zinc oxide nanorod arrays through sol electrophoretic deposition.
The Journal of Physical Chemistry, vol. 108, 2004, pp.19921-19931.
[4] Zhou WD, Wu X, Zhang YC. Solvothermal synthesis of hexagonal ZnO nanorods
and their photoluminescence properties. Materials Letters, vol. 61, 2007, pp. 2054-
2057.
[5] Li, D.X., Li, C.F., Wang, A.H., He, Q. and Li, J.B. (2010) monodispersity and shape
of gold nanoparticles formed by Hierarchical gold/copolymer nano-structures as the
Turkevich method. New J. Chem., 34: 2906.
[6] A. Takai, P.V. Kamat, Capture, store, and discharge, shuttling photogenerated
electrons across TiO2-silver interface. ACS Nano 5 (2011) 7369–7376.
[7] Chu, W., Environ. Sci. Technol. 1999, 33, 421-425.
[8] Davis, A. P. and Green, D. L., Environ. Sci. Technol. 1999, 33, 609-617.
[9] Calza, P. C.; Minero, C. and Pelizzeti, E., Environ. Sci. Technol. 1997, 31, 2198-
2203.
[10] Xie, Y. B. and Li, X. Z., J. Hazard. Mater. 2006, 138, 526-533.
[11] M. Schiavello, Heterogeneous photocatalysis - Vol. 3. John Wiley & Sons, 1st
Edition, 1997.
44
[12] M. R. Hoffmann, S. T. Martin, W. Choi and D. W. Bahnemann, Chem. Rev, 1995,
95,69.
[13] Li, Q.; Guo, B. D.; Yu, J. G.; Ran, J. R.; Zhang, B. H.; Yan, H. J. and Gong, J. R.,
J. Am. Chem. Soc. 2011, 133, 10878-10884.
[14] Wang, W. S.; Wang, D. H.; Qu, W. G.; Lu, L. Q. and Xu, A. W., J. Phys. Chem. C
2012, 116, 19893-19901.
[15] Bahadur, L.; Srivastava, P. Solar Energy Materials and Solar Cells 2003, 79, 235.
[16] Huang, T.; Murray, R. W. Langmuir 2002, 18, 7077.
[17] Armas, M. T.; Mederos, A.; Gili, P.; Dominguez, S.; Hernandez-Molina, R.;
Lorenzo, P.; Baran, E. J.; Araujo, M. L.; Brito, F. Polyhedron 2001,20, 799.
[18] Maeda, K.; Teramura, K.; Domen, K. Journal of Catalysis 2008,254,198.
[19] Liu, G.; Zhang, X.; Xu, Y.; Niu, X.; Zheng, L.; Ding, X. Chemosphere 2005, 59,
1367.
[20] Akpan, U. G.; Hameed, B. H. Journal of Hazardous Materials 2009, 170, 520.
[21] Buffat, P.; Borel, J. P. Physical Review A 1976,13, 2287.
[22] Haruta, M. CATTECH 2002, 6,102.
[23] Akita, T.; Lu, P.; Ichikawa, S.; Tanaka, K.; Haruta, M. Surface and Interface
Analysis 2001,31,73.
[24] Howard, A.; Mitchell, C. E. J.; Egdell, R. G. Surface Science 2002,515, L504.
[25] Bishop, P.T., Ashfield, L.J., Berzins, A., Boardman, A., Bioelectron., 23: 980-986.
Buche, V., Cookson, J., Gordon, R.J., Salcianu, C. and 19. Li, D.X., He, Q., Yang,
Y., Möhwald, H. and Li, J.B. (2008)
45
[26] Wallace, W.T. and Whetten, R.L. (2002) Coadsorption of CO 20. Xia, H. and
Wang, D. (2008) Fabrication of Macroscopic and O2 on selected gold clusters:
Evidence for efficient room- Freestanding Films of Metallic Nano-particle
Monolayers temperature CO generation. J. Am. Chem. Soc., 124: 7499. by
Interfacial Self-Assembly. Adv. Mater.,20: 4253-4256. 2.
[27] Zhou, X.C., Xu, W.L., Liu, G.K., Panda, D. and Chen, P. 21. Frens, G. (1973)
Controlled nucleation for the regulation of N (2010) Size-Dependent Catalytic Activity
and Dynamics of the particle size in monodisperse gold suspensions. Nat. Gold
Nanoparticles at the Single-Molecule Level. J. Am. Phys. Sci., 241: 20-22. Chem.
Soc., 132: 138.
[28] Lachheb, H., Puzenat, E., Houas, A., Ksibi, M., (2002). Photocatalytic degradation
of various types of dyes in water by UV-irradiated titania. Applied Catalysis B:
Environmental. 39: 75-90.
[29] Wang, Y.S., Zeng, L., Ren, X.F., Song, H., Wang, A.Q., 2010. Removal of methyl
violet from aqueous solutions using poly (acrylic acid-co-acrylamide)/attapulgite
composite. J. Environ. Sci. 22 (1), 7–14.
[30] McCreery RL. 2000. Raman spectroscopy for chemical analysis. Wiley-Interscience.
[31] Haynes CL, McFarland AD, Van Duyne RP. 2005. Surface-enhanced Raman
spectroscopy. Anal Chem 77(17): 338a-346a.
[32] Haynes CL, Yonzon CR, Zhang XY, Van Duyne RP. 2005b. Surface-enhanced
Raman sensors: early history and the development of sensors for quantitative
biowarfare agent and glucose detection. J Raman Spectrosc 36(6-7): 471-84.
46
[33] Doering WE, Nie S. 2002. Single-molecule and single-nanoparticle SERS: examining
the roles of surface active sites and chemical enhancement. J Phys Chem B 106(2):
311-7.
[34] Kneipp K, Kneipp H, Itzkan I, Dasari RR, Feld MS. 2002. Surface-enhanced Raman
scat tering and biophysics . J Phys -Condens Mat ter 14(18) : R597-624.
[35] Willets KA, Van Duyne RP. 2007. Localized surface plasmon resonance
spectroscopy and sensing. Annu Rev Phy Chem 58: 267-97.
[36] Bohren CF. Absorption and scattering of light by small particles. New York: Wiley;
1983.
[37] Kreibig U, Vollmer M. Optical properties of metal clusters. Springer; 1995.
[38] Dinish US, Balasundaram G, Chang Y-T, Olivo M. Actively targeted in vivo
multiplex detection of intrinsic cancer biomarkers using biocompatible SERS
nanotags. Sci Rep 2014, 4.
[39] Bindell, J. Scanning Electron Microscopy. Encyclopedia of Materials
Characterization: Surfaces, Interfaces, Thin Films, ed. Brundle, R. and C. Evans.
1992, Greenwich: Manning Publication Company
[40] Y. Feldman, G. L. Frey, M. Homyonfer, V. Lyakhovitskaya, L. Margulis, H. Cohen,
G. Hodes, J. L. Hutchison, and R. Tenne, “Bulk Synthesis of Inorganic Fullerenelike
MS 2 (M) Mo, W) from the Respective Trioxides and the Reaction Mechanism,"
Journal of the American Chemical Society, vol. 118, no. c, pp. 5362{5367, 1996.
[41] D. Ugarte, “Curling and closure of graphitic networks under electron-beam
irradiation," Nature, vol. 359, p. 707, 1992
[42] A. Selloni, Nature, 2008, 7, 613.
47
[43] A. Fujishima, K. Hashimoto and T. Watanabe, Photocatalysis: fundamentals and
applications; BKC, Tokyo, 1999.
[44] U. Diebold, N. Ruzycki, G. S. Herman and A. Selloni, Catal. Today, 2003, 85, 93.
[45] L. Kavan, M. Grätzel, S. E. Gilbert, C. Klemenz and H. J. Scheel, J. Am. Chem.
Soc., 1996, 118, 6716.
[46] T. Ohno, K. Sarakawa and M. Matsumura, New J. Chem., 2002, 26, 1167.
[47] I. M. Arabatzis, S. Antonaraki, T. Stergiopoulos, A. Hiskia, E. Papaconstantinou,
M. C. Bernard and P. Falaras, J. Photochem. Photobiol. A: Chem, 2002, 149, 237.
[48] Bunney, J.; Williamson, S.; Atkin, D.; Jeanneret, M.; Cozzolino, D.; Chapman, J.
The use of biosensors in food analysis. Curr. Res. Nutr. Food Sci. J. 2017, 5, 183–
195.
[49] Swinehart, D.F. The Beer-Lambert law. J. Chem. Educ. 1962, 39, 333.
[50] Ting-Yu Liu, Hung-Chou Liao, Chin-Ching Lin, Shang-Hsiu Hu, and San-Yuan
Chen. 2006. Biofunctional ZnO Nanorod Arrays Grown on Flexible Substrates.
Langmuir 2006, 22, 5804-5809.
[51] N.B. Li, L.M. Niu, H.Q. Luo, Microchim. Acta 153 (2006) 37.