摘要

量子點（QDs），膠體納米片提供許多功能，如生物標記，藥物遞送，光動力治療，納米診斷，太陽能電池和光子器件。由於顯示出高亮度發光並提供調整其尺寸和發射波長的可能性，QDs 的生物標記是當今納米材料最快的移動領域之一。

由於這種毒性和環境問題，開發CuInS2 / ZnS QDs 作為用於標記癌細胞的低毒納米材料變得重要。在這項工作中，CuInS2 / ZnS QDs 是通過熱膠體合成方法製成的。 QDs 通常被一層疏水分子覆蓋。因此，需要表面改性，例如塗覆或共軛親水或兩親分子，以將 QDs 從有機溶液轉化為水溶液用於生物應用。這裡，CuInS2 / ZnS QDs 塗覆有聚（乙二醇）甲基丙烯酸酯（PEGMA），其允許將含有極性的有機納米晶體從有機溶液轉移到水溶液中。 PEGMA 塗層的 CuInS2 / ZnS 顯示穩定的排放長達3週，並標記出具有清晰可見的紅色發光的人肝癌（HepG2）腫瘤細胞。

在本論文中，通過超聲處理的油溶性 QDs 可直接轉移到水相上，無需任何附加的表面活性劑，封端配體和表面塗層。進一步改進，顯示 CuInS2 / ZnS QDs 在直接製備而不含任何非極性溶劑的水中的分散體。最後，用水分散的 CuInS2 / ZnS QDs 染色的 HepG2 細胞共焦圖像體外評價證實轉移的 QDs 是無毒的，同時作為 HepG2 腫瘤細胞的標記試劑。

此外，證明了聚（馬來酸酐 - alt-1-十八碳烯）作為 CuInS2 / ZnS QDs 的封端配體和胺 - CuInS2 / ZnS QDs 的生物綴合的實用性。此外，還顯示了CuInS2 / ZnS QDs 在納米凝膠中的潛力的探索
关键词：CuInS2 / ZnS，量子点，超声波，肿瘤细胞
論文教授：Jia-Yaw Chang
標題：Professor of Nano Chemistry

Abstract
.
Quantum dots (QDs), which are colloidal nanocrsytals offer many functions such as biological labelling, drug delivery, photodynamic therapy, nanodiagnostic, and photonic devices. Because of displaying a bright luminescence and offering the possibility to tune their size and emission wavelength, biological labeling of QDs is one of the fastest moving field of nanomaterials today.

Due to alleged toxicity and environmental concern, developing CuInS2/ZnS QDs as low toxic nanomaterials for labeling cancer cells became important. In this work, CuInS2/ZnS QDs were made by the method of hot colloidal synthesis. QDs are usually covered by a layer of hydrophobic molecules. Thus, surface modification such as coating or conjugating hydrophilic or amphiphilic molecules is needed to convert QDs from organic to aqueous solution for biological application. Here, CuInS2/ZnS QDs was coated with Poly(ethylene glycol) methacrylate (PEGMA) which allows for transferring hydrophibically caped nanocrystals from organic to aqueous solution. The PEGMA coated CuInS2/ZnS showed stable emission for up to 3 weeks and labeled human liver carcinoma (HepG2) tumor cells with red luminescence were clearly visible.

In this thesis, CuInS2/ZnS QDs in oil phase can be directly transferred into water phase via sonication without any additional surfactant, capping ligand, or surface coating with polymers. Further improvement, CuInS2/ZnS QDs dispersion in water directly prepared without any non-polar solvent was revealed. Finally, HepG2 tumor cells confocal images stained with water-dispersed CuInS2/ZnS QDs are showing. In vitro evaluation has proven that the the transferred QDs are low-toxic along with being an efficient labelling agent to HepG2 tumor cells.

Furthermore Utility of poly(maleic anhydride-alt-1-octadecene) as capping ligand for CuInS2/ZnS QD and bioconjugation of Amine - CuInS2/ZnS QDs was demonstrated. In Addition the exploration of potential of CuInS2/ZnS QDs in nanogel was showed

Keywords : CuInS2/ZnS, Quantum Dots, Ultrasonic, tumor cells
Thesis supervisor : Jia Yaw-Chang
Title : Professor of Nano Chemistry

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