本研究使用葉酸做為還原劑及保護劑，藉此合成出葉酸-奈米金，由穿透式電子顯微鏡結果顯示，葉酸-奈米金粒徑為17±6nm，隨儲存時間越久奈米粒子呈現越高的介達電位，當儲存7天後，葉酸-奈米金粒徑介於45到50nm間，並具有良好的穩定性。
細胞攝入實驗中，我們觀察了骨癌細胞(UMR)與骨母細胞(7F2)對葉酸-奈米金的攝入情形，當在高濃度奈米金的情況下，進入細胞的金粒子數量變多、且奈米金進入細胞核數量也隨之變多。由於骨癌細胞有葉酸受體表現，導致骨癌細胞攝入及胞吐奈米金速度相較於骨母細胞都較快，此外，奈米粒子在骨母細胞中滯留數量較多滯留時間也較久。
體外細胞實驗結果指出，在培養一段時間後，在高濃度葉酸-奈米金會抑制細胞活性與蛋白質分泌，這應該是因為較多粒子進入細胞核中、且粒子在細胞中滯留。此外，只要濃度低於30μM時葉酸-奈米金對於後期骨細胞的礦化即不會產生影響。
表面拉曼光譜結果顯示，葉酸-奈米金對細胞的拉曼訊號有增強的效應，特別是骨分化蛋白與amide III的訊號強度更是被大幅的提升，此外，葉酸-奈米金對骨癌細胞中得拉曼訊號表現出較佳的增強效果，這是因為癌細胞對葉酸-奈米金具有更高的攝入效率。

In this research, synthesis the folic acid conjugated gold nanoparticle (FA-GNP) in a simple way by taking folic acid as reducing agent and protecting agent. According to the results of transmission electron microscopy (TEM), the particle size is 17 ± 6 nm. The zeta potential of the FA-GNP increases with the storage time. After the immersion in aqueous solution for 7 days, the particle size of FA-GNP is up to 45 to 50 nm with good stability.
UMR (Rat osteogenic sarcoma) and 7F2 (Osteoblast) are used for cellular uptake of FA-GNP. When the concentration of FA-GNP is high and more FA-GNP were uptake and more FA-GNP enter the nucleus. UMR cells uptake more FA-GNP than 7F2 due to the folate receptor expression of UMR. Besides, the FA-GNP shows longer retention time in UMR.
According to in vitro tests, the FA-GNP doesn’t affect the viability and phenotypes of cells at the early stage of culture. With concentrated FA-GNP, more FA-GNP enter into cells and inhibit the cell viability and production of extracellular matrix. Furthermore, as the concentration of FA-GNP were lower than 30μM, FA-GNP shows no effect on the late stage of osteogenic differemtiation.
The results from surface enhanced Raman spectroscopy (SERS) indicate that the FA-GNP would be able to enhance the intensity of the Raman spectra The FA-GNP was especially effective on the SERS peaks for amide Ⅲ bondings, the characteristic functional groups in osteogenic differentiation. More important, the Raman signals of osteosarcoma are enhanced more than osteoblasts, which is caused by the targeting effect of FA-GNP.

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