本研究以靜電紡絲法製備纖維，將幾丁聚醣(chitosan, Cs)和海藻酸(alginate, AL)以濕式電紡製備Cs-AL複合纖維；又將幾丁聚醣和聚環氧乙烷(PEO)以乾式電紡製備Cs-PEO纖維。再以交替浸泡的方式在材料表面進行磷灰石塗佈，沉積出羥基磷灰石(HAp)結晶。
在靜電紡絲製成部分，探討的參數包括濃度、流速，觀察參數對纖維外觀的影響。經磷灰石塗佈後的電紡纖維也接著進行物性分析，結晶在能量分散譜分析(EDS)、結構鑑定(FTIR)、X光繞射(XRD)測試後，結果與文獻上HAp數據相符。實驗也將材料置入於降解環境中，評估材料穩定性以瞭解材料使用時結構的破壞程度，結果顯示二種纖維的的降解度並不高，而以Cs-PEO-HAp降解程度較低。隨後進行的細胞相容性測試，觀察兩種纖維的細胞毒性、細胞增生，並觀察細胞貼附於材料表面的型態。Cs-PEO-HAp的細胞毒性與Cs-AL-HAp相差不大，對細胞均無毒性，但其增生略為優異。

In this study, we used electrospinning technique to produce two different fiber. Alginate was made fiber with Chitosan. Another fiber was prepared with Chitosan and PEO material. Then we coat apatite on our material to produce hydroxyapatite crystal by alternate soaking process.
The effect of electrospinning parameters, including concentration and flow rate, were investigated. Fiber after apatite coating was analyzed by EDS, FTIR, XRD and the crystal was confirmed as hydroxyapatite. Stability assessment showed that these two materials degraded less than 20% after 3 d in F12/DMEM/FBS or simulated body fluid. The results of cytocompatibility test show that these two materials were non-cytotoxicity, and that the proliferation of osteoblasts on Cs-PEO-HAp was slightly higher than that on Cs-AL-HAp.

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