表面電漿共振(Suface Plasmon Resonance, SPR)現象，被發現已經有近一百年的歷史，而近幾年來隨著生物醫學的發展，這項技術更是被廣泛應用在生物檢測上。由於它對不同折射率待測物有高靈敏度的特性，因此常常被用來做生物醫學檢測。常見的表面電漿共振量測方式有四種，角度調製、波長調製、強度調製以及相位調製，其中以相位靈敏度最高，因最大的相位變化發生在SPR曲線最窄處，也是探測電場相量最大處，所以為了取得反射光相位資訊，本論文使用低同調與高同調的光源干涉的表面電漿感測器架構來檢測微小核醣核酸的濃度變化。
本論文提出新穎性的方法以增強表面電漿共振空間相位生醫感測系統。由於低同調干涉波包具有穩定及明顯性，因此我們利用寬頻光源做為參考基準點，同時並聯高同調性雷射光源作為感測端，用以量測低濃度微小的相位變化。我們成功量測固定化Capture DNA前後之相位靈敏度，同時證明經硫醇修飾與硫基還原反應後的Capture DNA可以鍵結於金膜表面，並抓取Target DNA。另外專一性實驗，則證明我們使用之Capture DNA與Target DNA之間具有高度專一性。本架構量測microRNA-21之相位靈敏度為0.49 rad/µM。另外成功驗證雙通道同步取樣時間上的誤差，進而推算出檢測極限為0.63µM。

With the biomedical technique development, surface plasmon resonance (SPR), which phenomenon has been found for nearly a hundred years, is widely utilized in biological detection due to its high sensitivity. There are four kinds of SPR characterizations - angle, wavelength, intensity and phase modulation. Among them, the phase modulation demonstrates the highest sensitivity because the maximum phase change occurs in the SPR curve dip where the largest electric field is happening. In order to retrieve the reflected light phase information, the low coherence interferometry based SPR sensor is utilized to detect various concentrations of microRNA in this thesis.
In this thesis, a novel approach to improve SPR spatial phase biosensing is demonstrated. Because of the stability and resolvability of low coherence interferograms, the broad band source is interrogated with the laser beam reference interferogram to detect various analyte concentrations through the cascaded optical fiber low coherence interferometry (OFLCI).
We successfully demonstrated the capture DNA phase sensitivity before and after immobilization. The capture DNA could bond with the gold film surface through Thiol-modified Oligonucleotide reduction and then capture target DNA. Moreover, the excellent specificity between capture DNA and target DNA is also experimentally illustrated. Finally, the sensitivity is shown 0.49 (rad/µM). Furthermore, the error in the simultaneous sampling time of the two channels is utilized to successfully analyze the detection limit as 0.63 µM.

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