細胞膜對於水的通透性 (Cell-membrane permeability to water) 與細胞膜對於抗凍劑的通透性 (Cell-membrane permeability to cryoprotective agents) 是生物樣本進行最佳化冷凍保存 (Cryopreservation) 的關鍵資訊。此項研究開發了一種微渦流 (Mirco-vortex) 系統，利用微流體 (Microfluidics) 通道的擴展區域在低雷諾數下，被動形成的流體動力 (Hydrodynamic) 將感興趣的細胞捕獲並且維持在渦流中。被捕獲的細胞會保持懸浮狀態 (Suspension)，並隨著局部渦流的流線移動，因此，細胞被捕獲在該系統中避免了物理接觸 (Physical contact) 的情況發生，進一步支持細胞膜通透性的理論中利用細胞體積的圖形計算球型體積時，將其假設為100% 球形並且求出細胞膜活性表面積。因此，透過高速攝影中的即時細胞辨識系統，細胞膜通透性可以通過影像可視化追蹤單顆細胞並且取得其二維圖形，透過架設瞬態的滲透性梯度 (Osmotic gradient) 在細胞內 (Intracellular) 與細胞外 (Extracellular) 環境，計算響應細胞體積變化。本研究以急性 T 細胞淋巴瘤細胞系 (Jurkat) 為模型，來檢查新採用的微渦流技術，結果表明其數值略高於現有技術。我們的結果呈現高於使用基於物理接觸的細胞捕獲裝置，顯示基於非接觸式的量測顯著影響細胞膜通透性的活性表面積，提供一個提高對於細胞膜通透性量測準確性的新方法。

The permeabilities of cell-membrane to water (Lp) and cryoprotective agents (Ps) are the specific cellular information for achieving optimal cryopreservation of biological samples. In this research, a micro-vortex system was developed to practically capture the interesting cells in a low-Reynolds number hydrodynamic circulation passively formed at the expansion region of the microfluidic channel. Trapped cells remain suspended at the expansion region where the cells flow along with the streamline of the localized vortex. Therefore, the feature of none physical contact with the device structure supports the practical assumption of 100% sphericity and cell membrane active surface area, which is used to obtain images from the system to estimate the cell volume changes. In additional, cell membrane permeability was able to be accurately determined by direct visualization, with automatic cell recognition from high-speed videos, the transient cell-volume change curves response to a sudden osmotic gradient applied instantaneously (within 2 seconds) between the intracellular and extracellular environments. A cell line of acute T-cell lymphoma, Jurkat, was used as a model to examine the newly-adopted micro-vortex technology in this research, and our results indicate a moderately higher values than those in prior arts. Compared with those in the prior art using contact-based cell capture technology, it exhibits a significant influence of the active surface area on the measurement of cell membrane permeability.

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